Quantitative determination of guanethidine and other guanido-containing drugs in biological fluids by gas chromatography with flame ionization detection and multiple ion detection

1974 ◽  
Vol 46 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Jurgen H. Hengstmann ◽  
Fred C. Falkner ◽  
J. Throck. Watson ◽  
John. Oates
Keyword(s):  
Gas Chromatography ◽  
Quantitative Determination ◽  
Biological Fluids ◽  
Flame Ionization Detection ◽  
Ion Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Drugs In Biological Fluids

Simultaneous determination of methanol, acetaldehyde, acetone, and ethanol in human blood by gas chromatography with flame ionization detection

2013 ◽  
Vol 33 (1) ◽  
pp. 74-80 ◽  
Author(s):  
J Schlatter ◽  
F Chiadmi ◽  
V Gandon ◽  
P Chariot
Keyword(s):  
Gas Chromatography ◽  
Human Blood ◽  
Capillary Column ◽  
Sodium Tungstate ◽  
Biological Fluids ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Linear Correlations

Background: Methanol, acetaldehyde, acetone, and ethanol, which are commonly used as biomarkers of several diseases, in acute intoxications, and forensic settings, can be detected and quantified in biological fluids. Gas chromatography (GC)–mass spectrometry techniques are complex, require highly trained personnel and expensive materials. Gas chromatographic determinations of ethanol, methanol, and acetone have been reported in one study with suboptimal accuracy. Our objective was to improve the assessment of these compounds in human blood using GC with flame ionization detection. Methods: An amount of 50 µl of blood was diluted with 300 µl of sterile water, 40 µl of 10% sodium tungstate, and 20 µl of 1% sulphuric acid. After centrifugation, 1 µl of the supernatant was inje-cted into the gas chromatograph. We used a dimethylpolysiloxane capillary column of 30 m × 0.25 mm × 0.25 µm. Results: We observed linear correlations from 7.5 to 240 mg/l for methanol, acetaldehyde, and acetone and from 75 to 2400 mg/l for ethanol. Precision at concentrations 15, 60, and 120 mg/l for methanol, acetaldehyde, and acetone and 150, 600, and 1200 mg/ml for ethanol were 0.8–6.9%. Ranges of accuracy were 94.7–98.9% for methanol, 91.2–97.4% for acetaldehyde, 96.1–98.7% for acetone, and 105.5–111.6% for ethanol. Limits of detection were 0.80 mg/l for methanol, 0.61 mg/l for acetaldehyde, 0.58 mg/l for acetone, and 0.53 mg/l for ethanol. Conclusion: This method is suitable for routine clinical and forensic practices.

Determination of selected fatty acids in dried sweat spot using gas chromatography with flame ionization detection

2016 ◽  
Vol 39 (22) ◽  
pp. 4377-4383 ◽  
Author(s):  
Roman Kanďár ◽  
Petra Drábková ◽  
Lenka Andrlová ◽  
Adam Kostelník ◽  
Alexander Čegan
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization
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