Near-Infrared Heavy-Atom-Modified Fluorescent Dyes for Base-Calling in DNA-Sequencing Applications Using Temporal Discrimination

The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces base-calling accuracy and limits heterozygous allele detection, favoring use of dye-labeled primers for this purpose. We have discovered that the addition of a manganese salt to the PE Applied Biosystems dye-terminator sequencing kits overcomes these limitations for the older rhodamine dyes as well as the more recent dichloro-rhodamine dyes (dRhodamine and BigDyes). Addition of manganese to reactions containing dRhodamine-based dye terminators produced the highest base-calling accuracy. This combination resulted in the most uniform electropherogram profiles, superior to those produced by BigDye terminators and published for dye primers, and facilitated detection of heterozygous alleles.

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Synthesis of Silica Nanoparticles with Physical Encapsulation of Near-Infrared Fluorescent Dyes and Their Tannic Acid Coating

ACS Omega ◽

10.1021/acsomega.1c02204 ◽

2021 ◽

Author(s):

Yoshio Nakahara ◽

Yukiho Nakajima ◽

Soichiro Okada ◽

Jun Miyazaki ◽

Setsuko Yajima

Keyword(s):

Silica Nanoparticles ◽

Tannic Acid ◽

Near Infrared ◽

Fluorescent Dyes ◽

Acid Coating

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An expedient synthesis of N,N-dialkylamino-dihydroxanthene-pyrylium conjugated near-infrared fluorescent dyes

Tetrahedron Letters ◽

10.1016/j.tetlet.2015.12.010 ◽

2016 ◽

Vol 57 (3) ◽

pp. 317-320 ◽

Cited By ~ 15

Author(s):

Anthony Romieu ◽

Jean-Alexandre Richard

Keyword(s):

Near Infrared ◽

Fluorescent Dyes

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INDOCYANINE GREEN ENHANCED LAPAROSCOPIC CHOLECYSTECTOMY IN ACUTE PATIENTS: DO WE NEED A REVISION OF THE SAFETY PARADIGMS?

Romanian Medical Journal ◽

10.37897/rmj.2017.2.10 ◽

2017 ◽

Vol 64 (2) ◽

pp. 151-154

Author(s):

Catalin Alius ◽

◽

Eugen-Sebastian Gradinaru ◽

Adriana Elena Nica ◽

◽

...

Keyword(s):

Laparoscopic Cholecystectomy ◽

Augmented Reality ◽

Surgical Technique ◽

Indocyanine Green ◽

Near Infrared ◽

Fluorescent Dyes ◽

Operating Time ◽

Biliary Anatomy ◽

Early Laparoscopic Cholecystectomy ◽

Small Cohort

Introduction. Rapid developments in medical technology have allowed the incorporation of Indocyanine green (ICG) fluorescent cholangyography in the surgical technique armamentarium. The visualization of the biliary anatomy with augmented reality devices during surgery reduces complications and offer the perspective of challenging the safety paradigms which prohibited surgery in certain acute biliary conditions. Materials and methods. 43 consecutive patients were enrolled in a prospective interventional study and randomly divided into a cohort of 19 patients who had ICG injected prior to laparoscopic cholecystectomy and a cohort of 23 patients who received no fluorescent dye prior to surgery. In the ICG lot a Near Infrared Fluorescent System was used for the acquisition of fluorescent data in order to produce real time augmented reality imaging (ICG fluorescent cholangiography). The surgical technique and the indications for surgery were the same for the same in both cohorts of patients. Results and discussion. The cohort of patients receiving ICG had no complications and the mean operating time was 10 minutes less. The biliary anatomy was identified immediately in the ICG cohort with a specificity of 89.4% for the common bule duct and 73.6% for the cystic duct. In the non ICG cohort 21% of the CBDs and 43.4% of the cystic ducts were identified with difficulty during the procedure. Conclusion. We demonstrated in a small cohort of patients that early laparoscopic cholecystectomy is safe and can be performed quicker with the aid of fluorescent dyes. In order to challenge the safety paradigms around the early laparoscopic cholecystectomy a larger study is necessary.

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Optimization and functionalization of red-shifted rhodamine dyes

10.1101/2019.12.20.881227 ◽

2019 ◽

Cited By ~ 2

Author(s):

Jonathan B. Grimm ◽

Ariana N. Tkachuk ◽

Heejun Choi ◽

Boaz Mohar ◽

Natalie Falco ◽

...

Keyword(s):

Chemical Modification ◽

Near Infrared ◽

Fluorescent Dyes ◽

Poor Performance ◽

Biological Imaging ◽

Infrared Light ◽

Long Wavelength ◽

General Chemical ◽

Chemical Groups ◽

Rhodamine Dyes

ABSTRACTExpanding the palette of fluorescent dyes is vital for pushing the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.

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The Use of Fluorescent Anti-CEA Antibodies to Label, Resect and Treat Cancers: A Review

Biomolecules ◽

10.3390/biom11121819 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1819

Author(s):

Michael A. Turner ◽

Thinzar M. Lwin ◽

Siamak Amirfakhri ◽

Hiroto Nishino ◽

Robert M. Hoffman ◽

...

Keyword(s):

Near Infrared ◽

Fluorescent Dyes ◽

Review Article ◽

In Vivo Studies ◽

Murine Models ◽

Exclusion Criteria ◽

Major Barrier ◽

Tumor Margins ◽

Cancer Types

A major barrier to the diagnosis and effective treatment of solid-tumor cancers is the difficulty in detection and visualization of tumor margins in primary and metastatic disease. The use of fluorescence can augment the surgeon’s ability to detect cancer and aid in its resection. Several cancer types express carcinoembryonic antigen (CEA) including colorectal, pancreatic and gastric cancer. Antibodies to CEA have been developed and tagged with near-infrared fluorescent dyes. This review article surveyed the use of CEA antibodies conjugated to fluorescent probes for in vivo studies since 1990. PubMed and Google Scholar databases were queried, and 900 titles and abstracts were screened. Fifty-nine entries were identified as possibly meeting inclusion/exclusion criteria and were reviewed in full. Forty articles were included in the review and their citations were screened for additional entries. A total of 44 articles were included in the final review. The use of fluorescent anti-CEA antibodies has been shown to improve detection and resection of tumors in both murine models and clinically. The cumulative results indicate that fluorescent-conjugated anti-CEA antibodies have important potential to improve cancer diagnosis and surgery. In an emerging technology, anti-CEA fluorescent antibodies have also been successfully used for photoimmunotherapy treatment for cancer.

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