Comparative Elucidation of Cetuximab Heterogeneity on the Intact Protein Level by Cation Exchange Chromatography and Capillary Electrophoresis Coupled to Mass Spectrometry

2020 ◽  
Vol 92 (7) ◽  
pp. 5431-5438 ◽  
Author(s):  
Florian Füssl ◽  
Anne Trappe ◽  
Sara Carillo ◽  
Craig Jakes ◽  
Jonathan Bones
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Cation Exchange ◽  
Protein Level ◽  
Exchange Chromatography ◽  
Intact Protein ◽  
Cation Exchange Chromatography

Inductively coupled plasma mass spectrometry coupled to cation exchange chromatography for the determination of trace nickel in alkaline electrolyte

2020 ◽  
Vol 35 (7) ◽  
pp. 1295-1299
Author(s):  
Lukas Miner ◽  
Diane Beauchemin
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Inductively Coupled Plasma ◽  
Exchange Chromatography ◽  
Alkaline Solutions ◽  
Alkaline Electrolyte ◽  
Cation Exchange Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry

Cone corrosion by alkaline solutions with flow injection is prevented using cation-exchange chromatography coupled to inductively coupled plasma mass spectrometry.

HPLC with enzymatic detection as a candidate reference method for serum creatinine

1991 ◽  
Vol 37 (10) ◽  
pp. 1669-1675 ◽  
Author(s):  
K Linnet ◽  
I Bruunshuus
Keyword(s):  
Mass Spectrometry ◽  
Serum Creatinine ◽  
Cation Exchange ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Reference Method ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Ultraviolet Detection ◽  
Enzymatic Determination

Abstract We present a candidate Reference Method for determining the concentration of serum creatinine. The method is based on HPLC combined with enzymatic determination. Creatinine plus 14[C]creatinine is extracted by cation-exchange chromatography, subjected to reversed-phase HPLC, and finally quantified enzymatically. Enzymatic measurement ensures no interference from co-eluting compounds, which has been a problem for some reported HPLC assays relying on ultraviolet detection. The average corrected recovery was 100.1% (SEM = 1.1%; n = 15). The accuracy was verified by assaying five sera with target values determined by isotope dilution mass spectrometry. The total interassay CV was less than or equal to 2.5%. We applied the method to study the specificity of HPLC-ultraviolet detection, using 72 plasma samples from hospitalized patients; no interference was noted. Thus, HPLC-ultraviolet detection appears to be specific, provided that sample cleanup is based on cation-exchange chromatography. Our diode-array detector studies of peak homogeneity supported this conclusion. Still, combined HPLC-enzymatic determination ensures even greater accuracy, ranking close to that by isotope dilution mass spectrometry.

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