Single-Cell Isotope Dilution Analysis with LA–ICP–MS: A New Approach for Quantification of Nanoparticles in Single Cells

2020 ◽  
Vol 92 (21) ◽  
pp. 14339-14345 ◽  
Author(s):  
Ling-Na Zheng ◽  
Liu-Xing Feng ◽  
Jun-Wen Shi ◽  
Han-Qing Chen ◽  
Bing Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Isotope Dilution ◽  
Single Cells ◽  
Isotope Dilution Analysis ◽  
New Approach ◽  
Icp Ms

Correction of mass fractionation for isotope dilution analysis by MC-ICP-MS

2017 ◽  
Vol 51 (2) ◽  
pp. 157-165
Author(s):  
Le Zhang ◽  
Zhong-Yuan Ren ◽  
Jin-Long Ma ◽  
Xiao-Ping Xia
Keyword(s):  
Isotope Dilution ◽  
Isotope Dilution Analysis ◽  
Mass Fractionation ◽  
Icp Ms

A new approach reveals syncytia within the visceral musculature ofDrosophila melanogaster

Development ◽  
2001 ◽  
Vol 128 (13) ◽  
pp. 2517-2524 ◽  
Author(s):  
Robert Klapper ◽  
Sandra Heuser ◽  
Thomas Strasser ◽  
Wilfried Janning
Keyword(s):  
Drosophila Melanogaster ◽  
Single Cell ◽  
Cell Transplantation ◽  
Reporter Gene ◽  
Mononuclear Cells ◽  
Single Cells ◽  
New Approach ◽  
Transplantation Technique ◽  
Somatic Musculature ◽  
Somatic Muscles

In order to reveal syncytia within the visceral musculature of Drosophila melanogaster, we have combined the GAL4/UAS system with the single-cell transplantation technique. After transplantation of single cells from UAS-GFP donor embryos into ubiquitously GAL4-expressing recipients, the expression of the reporter gene was exclusively activated in syncytia containing both donor- and recipient-derived nuclei. In the first trial, we tested the system in the larval somatic musculature, which is already known to consist of syncytia. By this means we could show that most of the larval somatic muscles are generated by clonally non-related cells. Moreover, using this approach we were able to detect syncytia within the visceral musculature – a tissue that has previously been described as consisting of mononuclear cells. Both the longitudinal visceral musculature of the midgut and the circular musculature of the hindgut consist of syncytia and persist through metamorphosis. This novel application of the transplantation technique might be a powerful tool to trace syncytia in any organism using the GAL4/UAS system.

An approach for quantification of platinum distribution in tissues by LA-ICP-MS imaging using isotope dilution analysis

Talanta ◽  
2018 ◽  
Vol 178 ◽  
pp. 166-171 ◽  
Author(s):  
I. Moraleja ◽  
M.L. Mena ◽  
A. Lázaro ◽  
B. Neumann ◽  
A. Tejedor ◽  
...  
Keyword(s):  
Isotope Dilution ◽  
Isotope Dilution Analysis ◽  
Ms Imaging ◽  
Icp Ms

Quantitation of the Fe spatial distribution in biological tissue by online double isotope dilution analysis with LA-ICP-MS: a strategy for estimating measurement uncertainty

2016 ◽  
Vol 31 (1) ◽  
pp. 270-279 ◽  
Author(s):  
David N. Douglas ◽  
Jennifer O'Reilly ◽  
Ciaran O'Connor ◽  
Barry L. Sharp ◽  
Heidi Goenaga-Infante
Keyword(s):  
Spatial Distribution ◽  
Quantitative Analysis ◽  
Laser Ablation ◽  
Measurement Uncertainty ◽  
Isotope Dilution ◽  
Biological Tissue ◽  
Isotope Dilution Analysis ◽  
Icp Ms ◽  
On Line ◽  
Double Isotope

A strategy, systematically developed, is reported for the quantitative analysis of the Fe spatial distribution in biological tissue using laser ablation with ICP-MS and on-line double isotope dilution analysis.

Biosynthesis of isotopically enriched selenomethionine: application to its accurate determination in selenium-enriched yeast by isotope dilution analysis-HPLC-ICP-MS

2004 ◽  
Vol 19 (9) ◽  
pp. 1230-1235 ◽  
Author(s):  
L. Hinojosa Reyes ◽  
F. Moreno Sanz ◽  
P. Herrero Espílez ◽  
J. M. Marchante-Gayón ◽  
J. I. García Alonso ◽  
...  
Keyword(s):  
Isotope Dilution ◽  
Accurate Determination ◽  
Isotope Dilution Analysis ◽  
Icp Ms

Accurate determination of iron, copper and zinc in human serum by isotope dilution analysis using double focusing ICP-MS

1999 ◽  
Vol 14 (9) ◽  
pp. 1505-1510 ◽  
Author(s):  
Cristina Sariego Muñiz ◽  
Juan M. Marchante Gayón ◽  
J. Ignacio García Alonso ◽  
Alfredo Sanz-Medel
Keyword(s):  
Human Serum ◽  
Isotope Dilution ◽  
Accurate Determination ◽  
Isotope Dilution Analysis ◽  
Iron Copper ◽  
Copper And Zinc ◽  
Icp Ms ◽  
Double Focusing

scholarly journals On-line reverse isotope dilution analysis for spatial quantification of elemental labels used in immunohistochemical assisted imaging mass spectrometry via LA-ICP-MS

2019 ◽  
Vol 34 (2) ◽  
pp. 407-412 ◽  
Author(s):  
David Clases ◽  
Raquel Gonzalez de Vega ◽  
Paul A. Adlard ◽  
Philip A. Doble
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution ◽  
Imaging Mass Spectrometry ◽  
Isotope Dilution Analysis ◽  
Icp Ms ◽  
On Line

We present a novel on-line isotope dilution analysis (IDA) approach for the quantification of isotopically enriched metal labels used in immunohistochemical assisted imaging mass spectrometry.

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