Real-Time Analysis of Specific Binding between Apolipoprotein E Isoforms and Amyloid β-Peptide by Dual Polarization Interferometry

2020 ◽  
Author(s):  
Yu Wang ◽  
Yu Xue ◽  
Shuang Wang ◽  
Jianshe Huang ◽  
Xiurong Yang
Keyword(s):  
Apolipoprotein E ◽  
Real Time ◽  
Specific Binding ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Dual Polarization ◽  
Time Analysis ◽  
Polarization Interferometry ◽  
Real Time Analysis ◽  
Dual Polarization Interferometry

Real-Time Analysis of Binding Events between Different Aβ1–42 Species and Human Lilrb2 by Dual Polarization Interferometry

2017 ◽  
Vol 89 (4) ◽  
pp. 2606-2612 ◽  
Author(s):  
Tao Hu ◽  
Shuang Wang ◽  
Chuanxia Chen ◽  
Jian Sun ◽  
Xiurong Yang
Keyword(s):  
Real Time ◽  
Dual Polarization ◽  
Time Analysis ◽  
Polarization Interferometry ◽  
Real Time Analysis ◽  
Dual Polarization Interferometry

Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium(II) by Dual Polarization Interferometry

2020 ◽  
Vol 92 (14) ◽  
pp. 10007-10015 ◽  
Author(s):  
Yu Xue ◽  
Yu Wang ◽  
Shuang Wang ◽  
Mengxia Yan ◽  
Jianshe Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Real Time Detection

Apolipoprotein E includes a binding site which is recognized by several amyloidogenic polypeptides

2000 ◽  
Vol 349 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Marc H. BAUMANN ◽  
Jukka KALLIJÄRVI ◽  
Hilkka LANKINEN ◽  
Claudio SOTO ◽  
Matti HALTIA
Keyword(s):  
Apolipoprotein E ◽  
Binding Site ◽  
Late Onset ◽  
Specific Binding ◽  
Prion Diseases ◽  
Amyloid Β ◽  
Structural Motif ◽  
Amyloid Β Peptide ◽  
High Avidity ◽  
Amyloidogenic Protein

Inheritance of the apolipoprotein E (apoE) ϵ4 allele is a risk factor for late-onset Alzheimer's disease (AD). Biochemically apoE is present in AD plaques and neurofibrillary tangles of the AD brain. There is a high avidity and specific binding of apoE and the amyloid β-peptide (Aβ). In addition to AD apoE is also present in many other cerebral and systemic amyloidoses, Down's syndrome and prion diseases but the pathophysiological basis for its presence is still unknown. In the present study we have compared the interaction of apoE with Aβ, the gelsolin-derived amyloid fragment AGel183-210 and the amyloidogenic prion fragments PrP109-122 and PrP109-141. We show that, similar to Aβ, also AGel and PrP fragments can form a complex with apoE, and that the interaction between apoE and the amyloidogenic protein fragments is mediated through the same binding site on apoE. We also show that apoE increases the thioflavin-T fluorescence of PrP and AGel and that apoE influences the content of β-sheet conformation of these amyloidogenic fragments. Our results indicate that amyloids and amyloidogenic prion fragments share a similar structural motif, which is recognized by apoE, possibly through a single binding site, and that this motif is also responsible for the amyloidogenicity of these fragments.

Quantification of the effects of melittin on liposome structure

2005 ◽  
Vol 33 (5) ◽  
pp. 931-933 ◽  
Author(s):  
J. Popplewell ◽  
N. Freeman ◽  
S. Carrington ◽  
G. Ronan ◽  
C. McDonnell ◽  
...  
Keyword(s):  
Real Time ◽  
Rupture Process ◽  
Liquid Interface ◽  
Optical Technique ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Lipid Structures

An optical technique, dual-polarization interferometry, has been used to examine lipid structures at the solid/liquid interface. Changes in the lipid structures, in real time, were examined as a consequence of challenging them with a peptide (melittin) that is known to induce liposome rupture. This work suggests that it should be possible to obtain a better understanding of the detail of the melittin rupture process.

Real time, high resolution studies of protein adsorption and structure at the solid–liquid interface using dual polarization interferometry

2004 ◽  
Vol 16 (26) ◽  
pp. S2493-S2496 ◽  
Author(s):  
Neville J Freeman ◽  
Louise L Peel ◽  
Marcus J Swann ◽  
Graham H Cross ◽  
Andrew Reeves ◽  
...  
Keyword(s):  
High Resolution ◽  
Protein Adsorption ◽  
Real Time ◽  
Liquid Interface ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Solid Liquid Interface ◽  
Solid Liquid

Label-free Pb2+ detection on the layer-by-layer platform using real-time dual polarization interferometry

Talanta ◽  
2019 ◽  
Vol 202 ◽  
pp. 336-341 ◽  
Author(s):  
Shuang Wang ◽  
Shasha Lu ◽  
Jiahui Zhao ◽  
Jianshe Huang ◽  
Xiurong Yang
Keyword(s):  
Real Time ◽  
Layer By Layer ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry

Real-Time Study of Genomic DNA Structural Changes upon Interaction with Small Molecules Using Dual-Polarization Interferometry

2009 ◽  
Vol 81 (12) ◽  
pp. 4914-4921 ◽  
Author(s):  
Juan Wang ◽  
Xiaowen Xu ◽  
Zhanxia Zhang ◽  
Fan Yang ◽  
Xiurong Yang
Keyword(s):  
Small Molecules ◽  
Real Time ◽  
Genomic Dna ◽  
Structural Changes ◽  
Time Study ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry
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