Dual-Stimulus Responsive Near-Infrared Reversible Ratiometric Fluorescent and Photoacoustic Probe for In Vivo Tumor Imaging

2021 ◽  
Author(s):  
Xiao Liu ◽  
Xiangyang Gong ◽  
Jie Yuan ◽  
Xiaopeng Fan ◽  
Xingxing Zhang ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Stimulus Responsive

scholarly journals Spectroscopically Well-Characterized RGD Optical Probe as a Prerequisite for Lifetime-Gated Tumor Imaging

2011 ◽  
Vol 10 (6) ◽  
pp. 7290.2011.00018 ◽  
Author(s):  
Julia Eva Mathejczyk ◽  
Jutta Pauli ◽  
Christian Dullin ◽  
Joanna Napp ◽  
Lutz-F. Tietze ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Nude Mice ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Specific Binding ◽  
Optical Probe ◽  
Detection Sensitivity ◽  
Rgd Peptides ◽  
Emissive Probes

Labeling of RGD peptides with near-infrared fluorophores yields optical probes for noninvasive imaging of tumors overexpressing αvβ3 integrins. An important prerequisite for optimum detection sensitivity in vivo is strongly absorbing and highly emissive probes with a known fluorescence lifetime. The RGD-Cy5.5 optical probe was derived by coupling Cy5.5 to a cyclic arginine–glycine–aspartic acid–d-phenylalanine–lysine (RGDfK) peptide via an aminohexanoic acid spacer. Spectroscopic properties of the probe were studied in different matrices in comparison to Cy5.5. For in vivo imaging, human glioblastoma cells were subcutaneously implanted into nude mice, and in vivo fluorescence intensity and lifetime were measured. The fluorescence quantum yield and lifetime of Cy5.5 were found to be barely affected on RGD conjugation but dramatically changed in the presence of proteins. By time domain fluorescence imaging, we demonstrated specific binding of RGD-Cy5.5 to glioblastoma xenografts in nude mice. Discrimination of unspecific fluorescence by lifetime-gated analysis further enhanced the detection sensitivity of RGD-Cy5.5-derived signals. We characterized RGD-Cy5.5 as a strongly emissive and stable probe adequate for selective targeting of αvβ3 integrins. The specificity and thus the overall detection sensitivity in vivo were optimized with lifetime gating, based on the previous determination of the probes fluorescence lifetime under application-relevant conditions.

Noninvasive and Highly Multiplexed Five-Color Tumor Imaging of Multicore Near-Infrared Resonant Surface-Enhanced Raman Nanoparticles In Vivo

ACS Nano ◽  
2021 ◽  
Author(s):  
Jung Ho Yu ◽  
Idan Steinberg ◽  
Ryan M. Davis ◽  
Andrey V. Malkovskiy ◽  
Aimen Zlitni ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging

2019 ◽  
Vol 95 (5) ◽  
pp. 475-489
Author(s):  
Mona Doshi ◽  
Daniel A. Nierenberg ◽  
Orielyz Flores-Fernandez ◽  
Pragney Deme ◽  
Edilu Becerra ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Tumor Imaging

Targeted molecular imaging of prostate cancer using tumor endothelium homing Arg-Arg-Leu peptides

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 14582-14582 ◽  
Author(s):  
D. O. Henry ◽  
S. C. Chen ◽  
M. K. Wong
Keyword(s):  
Prostate Cancer ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Specific Binding ◽  
Time Lapse ◽  
Human Prostate ◽  
Animal Imaging ◽  
Tumor Endothelium ◽  
Unique Cell

14582 Background: Tumor endothelial cells express a specific and unique cell surface signature. We have previously reported on the discovery of tumor endothelial cell (TDEC) binding peptides that possess the amino acid sequence Arg-Arg-Leu (RRL). We now show specific binding of this synthetic peptide to both surgically resected and experimental human prostate adenocarcinoma tumors thereby allowing for tumor imaging. Methods and Results: Cryosections from human lung, colon, renal, breast and prostate cancers were immunohistochemically processed to reveal the relationship of RRL-peptide staining in relation to Factor VIII labeled tumor vasculature. The RRL-peptide tightly co-localizes onto tumor endothelium within human prostate adenocarcinomas, and did not stain the tumor cells proper. Alexa 680, 800 are two near-infrared dyes that can be conjugated to RRL peptide and which emit at a wavelength unquenched by biologic tissue, thus permitting whole animal imaging. Intravenous administration of RRL-peptide-Alexa chimera to human PC3 prostate tumor bearing mice or TRAMP (transgenic prostate cancer) mice results in the fluorescent visualization of tumors within the intact animal through the prolonged intra-tumor retention of the RRL-dye complex as compared to controls. Direct time-lapse intravital microscopy of such tumors show real-time tumor vascular binding of the RRL peptide. Whole animal imaging revealed the tumor and did not show an appreciable image signal at any other site or organ. Conclusion: The RRL peptide homes to prostate vasculature and is useful for the specific detection of experimental prostate tumors in vivo. Further development of the RRL-peptide into a drug delivery and imaging agent is underway. No significant financial relationships to disclose.

In vivo tumor imaging by a γ-glutamyl transpeptidase-activatable near-infrared fluorescent probe

2018 ◽  
Vol 410 (26) ◽  
pp. 6771-6777 ◽  
Author(s):  
Lihong Li ◽  
Wen Shi ◽  
Xiaofeng Wu ◽  
Xiaohua Li ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Glutamyl Transpeptidase

scholarly journals Noninvasive and Highly Multiplexed Five-Color Tumor Imaging of Near-Infrared Resonant Surface-Enhanced Raman Nanoparticles In Vivo

2020 ◽  
Author(s):  
Jung-Ho Yu ◽  
Idan Steinberg ◽  
Ryan Miller Davis ◽  
Andrey V. Malkovskiy ◽  
Aimen Zlitni ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Ex Vivo ◽  
Spectral Width ◽  
Raman Imaging ◽  
Multiple Tumor ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Longitudinal Imaging

In vivo multiplexed imaging aims for noninvasive analysis of multiple tumor biomarkers. While most of the preclinical imaging has provided a number of multiplexing channels up to three, Raman imaging with surface-enhanced Raman scattering (SERS) nanoparticles was suggested to offer higher multiplexing capability originating from their narrow spectral width. However, the multiplexed Raman imaging is still in its infancy for visualizing tumors with both sufficient multiplicity and high sensitivity concurrently. Here we create multispectral palettes of gold core-near-infrared (NIR) resonant Raman dyes-silica shell SERS (NIR-SERRS) nanoparticles, which provide both the high multiplicity and fluorescence-comparable sensitivity. With the NIR-SERRS nanoparticle palettes, we demonstrate noninvasive and five-plex ratiometric Raman imaging of tumors in living mice. Furthermore, we perform noninvasive and longitudinal imaging of the five-color nanoparticles in the tumors, which is not feasible with current ex vivo multiplexing platforms, demonstrating great potential for noninvasive assessment of multiple biological targets within tumors.

Glucose-functionalized near-infrared Ag2Se quantum dots with renal excretion ability for long-term in vivo tumor imaging

2019 ◽  
Vol 7 (38) ◽  
pp. 5782-5788 ◽  
Author(s):  
Xiao-Lei Ge ◽  
Biao Huang ◽  
Zhi-Ling Zhang ◽  
Xiaolan Liu ◽  
Man He ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Renal Excretion ◽  
Tumor Imaging

Non-toxic and long-term fluorescent probes for tumor imaging are in urgent need for non-invasively obtaining information about tumor genesis and metastasis in vivo.

Human serum albumin (HSA) coated liposomal indocyanine green for in vivo tumor imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (18) ◽  
pp. 15220-15225 ◽  
Author(s):  
Siqin Chen ◽  
Gongjie Yu ◽  
Bo Zhang ◽  
Yinsong Wang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Fluorescent Nanoprobe

In this study, a near-infrared (NIR) fluorescent nanoprobe based on indocyanine green (ICG) was synthesized.

scholarly journals Assessment of the Theranostic Potential of Gold Nanostars—A Multimodal Imaging and Photothermal Treatment Study

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2112 ◽  
Author(s):  
Antoine D’Hollander ◽  
Greetje Vande Velde ◽  
Hilde Jans ◽  
Bram Vanspauwen ◽  
Elien Vermeersch ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Photoacoustic Imaging ◽  
Tumor Therapy ◽  
Infrared Region ◽  
Tumor Cell Death ◽  
Gold Nanostars ◽  
Theranostic Agents

Gold nanoparticles offer the possibility to combine both imaging and therapy of otherwise difficult to treat tumors. To validate and further improve their potential, we describe the use of gold nanostars that were functionalized with a polyethyleneglycol-maleimide coating for in vitro and in vivo photoacoustic imaging (PAI), computed tomography (CT), as well as photothermal therapy (PTT) of cancer cells and tumor masses, respectively. Nanostar shaped particles show a high absorption coefficient in the near infrared region and have a hydrodynamic size in biological medium around 100 nm, which allows optimal intra-tumoral retention. Using these nanostars for in vitro labeling of tumor cells, high intracellular nanostar concentrations could be achieved, resulting in high PAI and CT contrast and effective PTT. By injecting the nanostars intratumorally, high contrast could be generated in vivo using PAI and CT, which allowed successful multi-modal tumor imaging. PTT was successfully induced, resulting in tumor cell death and subsequent inhibition of tumor growth. Therefore, gold nanostars are versatile theranostic agents for tumor therapy.

A fluorinated aza-BODIPY derivative for NIR fluorescence/PA/19F MR tri-modality in vivo imaging

2019 ◽  
Vol 55 (42) ◽  
pp. 5851-5854 ◽  
Author(s):  
Lianhua Liu ◽  
Yaping Yuan ◽  
Yuqi Yang ◽  
Michael T. McMahon ◽  
Shizhen Chen ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Small Molecule ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Near Infrared Fluorescence ◽  
Highly Efficient ◽  
Nir Fluorescence

A fluorinated aza-BODIPY derivative BDPF was developed as a small molecule contrast agent, which displayed highly efficient near infrared fluorescence/photoacoustic/19F MR tri-modality tumor imaging.

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