Photoswitch-Based Fluorescence Encoding of Microspheres in a Limited Spectral Window for Multiplexed Detection

2022 ◽  
Author(s):  
Chao Guo ◽  
Jingying Zhai ◽  
Yifu Wang ◽  
Xinfeng Du ◽  
Zige Wang ◽  
...  
Keyword(s):  
Spectral Window ◽  
Multiplexed Detection

Ruthenium Complexes for 1- and 2-Photon Photodynamic Therapy: From In Silico Prediction to In Vivo Applications

2020 ◽  
Author(s):  
Johannes Karges ◽  
Shi Kuang ◽  
Federica Maschietto ◽  
Olivier Blacque ◽  
Ilaria Ciofini ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Silico ◽  
Aqueous Solubility ◽  
The Body ◽  
Photon Absorption ◽  
Multicellular Tumor Spheroids ◽  
Spectral Window ◽  
Photon Excitation ◽  
Polypyridine Complexes

<div>The use of photodynamic therapy (PDT) against cancer has received increasing attention overthe recent years. However, the application of the currently approved photosensitizers (PSs) is somehow limited by their poor aqueous solubility, aggregation, photobleaching and slow clearance from the body. To overcome these limitations, there is a need for the development of new classes of PSs with ruthenium(II) polypyridine complexes currently gaining momentum. However, these compounds generally lack significant absorption in the biological spectral window, limiting their application to treat deep-seated or large tumors. To overcome this drawback, ruthenium(II) polypyridine complexes designed in silico with (E,E’)-4,4´-bisstyryl 2,2´-bipyridine ligands showed impressive 1- and 2-Photon absorption up to a magnitude higher than the ones published so far. While non-toxic in the dark, these compounds were found phototoxic in various 2D monolayer cells, 3D multicellular tumor spheroids and be able to eradicate a multiresistant tumor inside a mouse model upon clinically relevant 1-Photon and 2 Photon excitation.</div>

The Interplay of Open-Shell Spin-Coupling and Jahn-Teller Distortion in Benzene Radical Cation Probed by X-ray Spectroscopy

2020 ◽  
Author(s):  
Marta L. Vidal ◽  
Michael Epshtein ◽  
Valeriu Scutelnic ◽  
Zheyue Yang ◽  
Tian Xue ◽  
...  
Keyword(s):  
High Harmonic ◽  
Open Shell ◽  
Spin Coupling ◽  
High Symmetry ◽  
Teller Distortion ◽  
Spectral Window ◽  
X Ray ◽  
Jahn Teller ◽  
X Ray Absorption ◽  
Jahn Teller Distortion

We report a theoretical investigation and elucidation of the x-ray absorption spectra of neutral benzene and of the benzene cation. The generation of the cation by multiphoton ultraviolet (UV) ionization as well as the measurement of<br>the carbon K-edge spectra of both species using a table-top high-harmonic generation (HHG) source are described in the companion experimental paper [M. Epshtein et al., J. Phys.<br>Chem. A., submitted. Available on ChemRxiv]. We show that the 1sC -> pi transition serves as a sensitive signature of the transient cation formation, as it occurs outside of the spectral window of the parent neutral species. Moreover, the presence<br>of the unpaired (spectator) electron in the pi-subshell of the cation and the high symmetry of the system result in significant differences relative to neutral benzene in the spectral features associated with the 1sC ->pi* transitions. High-level calculations using equation-of-motion coupled-cluster theory provide the interpretation of the experimental spectra and insight into the electronic structure of benzene and its cation.<br>The prominent split structure of the 1sC -> pi* band of the cation is attributed to the interplay between the coupling of the core -> pi* excitation with the unpaired electron<br>in the pi-subshell and the Jahn-Teller distortion. The calculations attribute most of<br>the splitting (~1-1.2 eV) to the spin coupling, which is visible already at the Franck-Condon structure, and estimate the additional splitting due to structural relaxation to<br>be around ~0.1-0.2 eV. These results suggest that x-ray absorption with increased resolution might be able to disentangle electronic and structural aspects of the Jahn-Teller<br>effect in benzene cation.<br>

scholarly journals P052 Nuclear magnetic resonance metabolomic profiling of IBD patients under anti-TNF treatment. Are the pathways network deregulated?

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S160-S160
Author(s):  
S Notararigo ◽  
M Martin-Pastor ◽  
J E Dominguez Munoz ◽  
M Barreiro-de Acosta
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nmr Spectra ◽  
Mononuclear Cells ◽  
High Rate ◽  
Resonance Spectroscopy ◽  
Serum Samples ◽  
Spectral Window ◽  
Metabolomic Study ◽  
Nuclear Magnetic

Abstract Background The deregulation of immune system cell response implies loss of T-cell apoptosis, high rate of proinflammatory cytokines production and subsequent exacerbate activation of TNF-α pathway. The use of biologic antibody decrease inflammation rate and symptoms, but it remains unclear if it has a direct effect on the pathways activation/inactivation on peripheral blood mononuclear cells (PBMCs). The aim of this study is evaluate the role of nuclear magnetic resonance spectroscopy (NMR) applied to the metabolomic study of serum samples isolated from fresh blood from inflammatory bowel disease (IBD) patients under IFX treatment to understand the activated/inactivated pathways of PBMCs. Methods A case–control study was performed. Inclusion criteria were IBD patients under IFX treatment. Blood samples were obtained in Crohn’s disease (CD) and ulcerative colitis (UC) patients before IFX and in healthy controls (CTRL). CD patients were divided into subgroups according to the gut affected, in Ileocolic (IC), ileum and colon. NMR samples of the serum were collected and measured according to Standard Operation Procedures. Three types of NMR spectra were measured for each serum sample (1Hnoepresat, 1Hcpmgpresat and 1HDfilterpresat). The signal in each NMR spectrum was integrated in a series of equidistant little portion of the spectrum called buckets of a constant width of 0.04 ppm, covering the complete 1H NMR spectral window from −5 to 14 ppm. Buckets in regions depleted from signal at the two extremes of the spectrum were discarded as well as those in the proximity of the water peak at ca. 4.7 ppm which was affected by the presaturation. The vectors corresponding to a number of samples of two or more groups can be rapidly analysed using Multivariant Statistical Analysis methods. Results Twenty-two IBD patients (12 CD and nine UC) were included, 10 CTRL were also included. The metabolomic analyses of the NMR spectra of the serum of the different patients and control groups by the fingerprinting and targeting profiling strategies provided OPLS-DA statistical models (Figure 1) that permitted the successful classification of certain groups of samples which are summarised in Table 1. Conclusion The results of this pilot NMR metabolomic study of serum samples of IBD found a series of spectral fingerprints that are able to discriminate between groups of patients CTRL and CD, which underlines its potential use for the diagnosis of the disease.

One-step fast and label-free imaging array for multiplexed detection of trace avian influenza viruses

2021 ◽  
pp. 338645
Author(s):  
Dagang Jiang ◽  
Yafei Tian ◽  
Yujiao Zhang ◽  
Xueyun Lu ◽  
Dan Xiao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza Viruses ◽  
Label Free ◽  
Avian Influenza Viruses ◽  
Multiplexed Detection ◽  
Imaging Array ◽  
One Step

scholarly journals Multiplexed Detection of Analytes on Single Test Strips with Antibody‐Gated Indicator‐Releasing Mesoporous Nanoparticles

2020 ◽  
Vol 59 (52) ◽  
pp. 23862-23869 ◽  
Author(s):  
Estela Climent ◽  
Mustafa Biyikal ◽  
Delia Gröninger ◽  
Michael G. Weller ◽  
Ramón Martínez‐Máñez ◽  
...  
Keyword(s):  
Single Test ◽  
Multiplexed Detection ◽  
Test Strips ◽  
Mesoporous Nanoparticles

scholarly journals Retrieving Sun-Induced Chlorophyll Fluorescence from Hyperspectral Data with TanSat Satellite

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4886
Author(s):  
Shilei Li ◽  
Maofang Gao ◽  
Zhao-Liang Li
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Spectrum ◽  
Singular Vector ◽  
Low Frequency ◽  
Information Criterion ◽  
Hyperspectral Data ◽  
High Spectral Resolution ◽  
Remotely Sensed Data ◽  
Spectral Window ◽  
Vector Decomposition

A series of algorithms for satellite retrievals of sun-induced chlorophyll fluorescence (SIF) have been developed and applied to different sensors. However, research on SIF retrieval using hyperspectral data is performed in narrow spectral windows, assuming that SIF remains constant. In this paper, based on the singular vector decomposition (SVD) technique, we present an approach for retrieving SIF, which can be applied to remotely sensed data with ultra-high spectral resolution and in a broad spectral window without assuming that the SIF remains constant. The idea is to combine the first singular vector, the pivotal information of the non-fluorescence spectrum, with the low-frequency contribution of the atmosphere, plus a linear combination of the remaining singular vectors to express the non-fluorescence spectrum. Subject to instrument settings, the retrieval was performed within a spectral window of approximately 7 nm that contained only Fraunhofer lines. In our retrieval, hyperspectral data of the O2-A band from the first Chinese carbon dioxide observation satellite (TanSat) was used. The Bayesian Information Criterion (BIC) was introduced to self-adaptively determine the number of free parameters and reduce retrieval noise. SIF retrievals were compared with TanSat SIF and OCO-2 SIF. The results showed good consistency and rationality. A sensitivity analysis was also conducted to verify the performance of this approach. To summarize, the approach would provide more possibilities for retrieving SIF from hyperspectral data.

Early Multiplexed Detection of Cirrhosis using Giant Magnetoresistive Biosensors with Protein Biomarkers

ACS Sensors ◽  
2020 ◽  
Vol 5 (10) ◽  
pp. 3049-3057
Author(s):  
Elaine Ng ◽  
An K. Le ◽  
Mindie H. Nguyen ◽  
Shan X. Wang
Keyword(s):  
Protein Biomarkers ◽  
Multiplexed Detection
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