Visualization of in Vivo Hydrogen Sulfide Production by a Bioluminescence Probe in Cancer Cells and Nude Mice

ABSTRACTCysteine desulfuration is one of the main ways for hydrogen sulfide (H2S) generation in cells and is usually conducted by cystathionine γ-lyase. Herein, we describe a newly discovered deep-sea bacterial threonine dehydratase (psTD), which is surprisingly discovered to drive L-cysteine desulfuration. The mechanisms of psTD catalyzing cysteine desulfuration towards H2S production are first clarified in vitro and in vivo through a combination of genetic and biochemical methods. Furthermore, based on the solved structures of psTD and its various mutants, two or three pockets are found in the active site of psTD, and switch states between inward and outward orientation of a key amino acid R77 determine the open or close status of Pocket III for small molecule exchanges, which further facilitates cysteine desulfuration. Our results reveal the functional diversity and structural specificity of psTD towards L-cysteine desulfuration and H2S formation. Given the broad distribution of psTD homologs in different bacteria, we speculate that some threonine dehydratases have evolved a novel function towards cysteine desulfuration, which benefits the producer to utilize cysteine as a sulfur source for better adapting external environments.

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Influence of pH of TSI medium on the detection of hydrogen sulfide production by Campylobacter hyointestinalis

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.2006.02097.x ◽

2007 ◽

Vol 44 (5) ◽

pp. 544-549 ◽

Cited By ~ 2

Author(s):

M. Ma ◽

T. Amano ◽

M. Enokimoto ◽

T. Yano ◽

K.K. Moe ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Sulfide Production ◽

Hydrogen Sulfide Production ◽

Influence Of Ph

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Effect of theophylline on endogenous hydrogen sulfide production in patients with COPD

Pulmonary Pharmacology & Therapeutics ◽

10.1016/j.pupt.2006.11.002 ◽

2008 ◽

Vol 21 (1) ◽

pp. 40-46 ◽

Cited By ~ 20

Author(s):

Ya-Hong Chen ◽

Wan-Zhen Yao ◽

Yan-Ling Ding ◽

Bin Geng ◽

Ming Lu ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Sulfide Production ◽

Hydrogen Sulfide Production

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Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

BioMed Research International ◽

10.1155/2016/4897986 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Wei Xin Cai ◽

Li Wu Zheng ◽

Li Ma ◽

Hong Zhang Huang ◽

Ru Qing Yu ◽

...

Keyword(s):

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Nude Mice ◽

Tongue Cancer ◽

Cancer Cell Lines ◽

Fluorescent Imaging ◽

Cell Phenotype ◽

Human Tongue

Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.

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