Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies

2017 ◽  
Vol 89 (7) ◽  
pp. 4007-4012 ◽  
Author(s):  
Martin Dippong ◽  
Peter Carl ◽  
Christine Lenz ◽  
Jörg A. Schenk ◽  
Katrin Hoffmann ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Single Cell ◽  
Cell Sorting ◽  
Cell Selection ◽  
Fluorescence Activated Cell Sorting ◽  
Selection Of

scholarly journals Gezielte Zellsortierung in der Einzelzellgenomik

BIOspektrum ◽  
2021 ◽  
Vol 27 (3) ◽  
pp. 274-276
Author(s):  
Morgan S. Sobol ◽  
Anne-Kristin Kaster
Keyword(s):  
Dark Matter ◽  
Single Cell ◽  
Cell Sorting ◽  
Genetic Information ◽  
Fluorescent Labeling ◽  
Single Cell Genomics ◽  
Rare Biosphere ◽  
Prokaryotic Genomes ◽  
Selection Of

AbstractSingle cell genomics (SCG) can provide reliable context for assembled genome fragments on the level of individual prokaryotic genomes and has rapidly emerged as an essential complement to cultivation-based and metagenomics research approaches. Targeted cell sorting approaches, which enable the selection of specific taxa by fluorescent labeling, compatible with subsequent single cell genomics offers an opportunity to access genetic information from rare biosphere members which would have otherwise stayed hidden as microbial dark matter.

scholarly journals Large particle fluorescence-activated cell sorting enables high quality single cell RNA-sequencing and functional analysis of adult cardiomyocytes

2019 ◽  
Author(s):  
Suraj Kannan ◽  
Matthew Miyamoto ◽  
Brian Lin ◽  
Renjun Zhu ◽  
Sean Murphy ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Cell Sorting ◽  
Large Particle ◽  
Fluorescence Activated Cell Sorting ◽  
High Quality ◽  
Adult Cardiomyocytes ◽  
Single Cell Rna Sequencing ◽  
Mitochondrial Transcripts ◽  
Manual Methods

ABSTRACTRationaleSingle cell RNA sequencing (scRNA-seq) has emerged as a powerful tool to profile the transcriptome at single cell resolution, enabling comprehensive analysis of cellular trajectories and heterogeneity during development and disease. However, the use of scRNA-seq remains limited in cardiac pathology owing to technical difficulties associated with the isolation of single adult cardiomyocytes (CMs).ObjectiveWe investigated the capability of large-particle fluorescence-activated cell sorting (LP-FACS) for isolation of viable single adult CMs.Methods and ResultsWe found that LP-FACS readily outperforms conventional FACS for isolation of struturally competent CMs, including large CMs. Additionally, LP-FACS enables isolation of fluorescent CMs from mosaic models. Importantly, the sorted CMs allow generation of high-quality scRNA-seq libraries. Unlike CMs isolated via previously utilized fluidic or manual methods, LP-FAC-isolated CMs generate libraries exhibiting normal levels of mitochondrial transcripts. Moreover, LP-FACS isolated CMs remain functionally competent and can be studied for contractile properties.ConclusionsOur study enables high quality dissection of adult CM biology at single-cell resolution.

scholarly journals Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies

Blood ◽  
1983 ◽  
Vol 62 (1) ◽  
pp. 124-132 ◽  
Author(s):  
RG Andrews ◽  
B Torok-Storb ◽  
ID Bernstein
Keyword(s):  
Stem Cells ◽  
Monoclonal Antibodies ◽  
Cell Sorting ◽  
Bone Marrow Cells ◽  
Antigenic Determinants ◽  
Fluorescence Activated Cell Sorting ◽  
Monocytic Cells ◽  
Colony Forming Units ◽  
Potential Applications ◽  
A Minor

Abstract Within the hematopoietic system, monoclonal antibodies reactive with antigenic determinants, expressed in a lineage- and stage-restricted fashion, can be used to map myeloid differentiation. We have generated a series of monoclonal antibodies that reacts with myeloid-associated determinants on committed myeloid stem cells and their progeny. Their reactivity with peripheral blood cells was identified by immunofluorescence assays, with bone marrow cells by fluorescence- activated cell sorting, and with committed hematopoietic progenitor cells by both cytotoxic assays and fluorescence-activated cell sorting. Antibody 1G10, which has previously been reported to react with cells of the granulocytic lineage and with a minor subset of mature monocytes, was shown to react with granulocyte-macrophage colony- forming units (CFU-GM). Three antibodies not previously characterized (T5A7, L4F3, L1B2) were shown to react with both granulocytic and monocytic cells and in fluorescence-activated cell sorting studies to detectably stain granulocytic cells at different stages of maturation. These three antibodies also react with CFU-GM, two (L4F3 and L1B2) reacting with all CFU-GM, while T5A7 reacts with only a portion of the day 7 CFU-GM. Antibody L4F3 also reacts with a portion of erythroid burst-forming units (BFU-E). In contrast, the previously reported antibody 5F1, which reacts with monocytic cells, nucleated erythroid cells, and platelets, was shown to react with erythroid colony-forming units (CFU-E). Potential applications of these antibodies to studies of normal and malignant hematopoiesis are discussed.

Selection of aptamers against Lactoferrin based on silver enhanced and fluorescence-activated cell sorting

Talanta ◽  
2019 ◽  
Vol 193 ◽  
pp. 110-117 ◽  
Author(s):  
Fang Yu ◽  
Hui Li ◽  
Wei Sun ◽  
Yaju Zhao ◽  
Danke Xu ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Fluorescence Activated Cell Sorting ◽  
Selection Of

Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

10.3791/60148 ◽  
2019 ◽  
Author(s):  
Dehia Sahmi-Bounsiar ◽  
Paulo Victor de Miranda Boratto ◽  
Graziele Pereira Oliveira ◽  
Jacques Yaacoub Bou Khalil ◽  
Bernard La Scola ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Sorting ◽  
Alternative Strategy ◽  
Giant Virus ◽  
Fluorescence Activated Cell Sorting ◽  
Mixture Separation

Fluorescence activated cell sorting via a focused traveling surface acoustic beam

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3176-3185 ◽  
Author(s):  
Zhichao Ma ◽  
Yinning Zhou ◽  
David J. Collins ◽  
Ye Ai
Keyword(s):  
Acoustic Wave ◽  
Single Cell ◽  
Surface Acoustic Wave ◽  
Cell Sorting ◽  
Wave Beam ◽  
Single Cell Level ◽  
Acoustic Beam ◽  
Fluorescence Activated Cell Sorting ◽  
Cell Level

Fluorescence activated sorting at the single cell level using a highly focused traveling surface acoustic wave beam (∼50 μm).

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