scholarly journals Rates of Chemical Cleavage of DNA and RNA Oligomers Containing Guanine Oxidation Products

2015 ◽  
Vol 28 (6) ◽  
pp. 1292-1300 ◽  
Author(s):  
Aaron M. Fleming ◽  
Omar Alshykhly ◽  
Judy Zhu ◽  
James G. Muller ◽  
Cynthia J. Burrows
Keyword(s):  
Oxidation Products ◽  
Guanine Oxidation ◽  
Chemical Cleavage ◽  
Dna And Rna ◽  
Rna Oligomers

scholarly journals Generation, repair and replication of guanine oxidation products

2017 ◽  
Vol 39 (1) ◽  
Author(s):  
Katsuhito Kino ◽  
Masayo Hirao-Suzuki ◽  
Masayuki Morikawa ◽  
Akane Sakaga ◽  
Hiroshi Miyazawa
Keyword(s):  
Oxidation Products ◽  
Guanine Oxidation

Synthesis, physico-chemical and biological properties of DNA and RNA oligonucleotides containing short alkylamino internucleotide bond

2011 ◽  
Vol 76 (12) ◽  
pp. 1471-1486
Author(s):  
Milena Sobczak ◽  
Katarzyna Kubiak ◽  
Magdalena Janicka ◽  
Malgorzata Sierant ◽  
Barbara Mikolajczyk ◽  
...  
Keyword(s):  
Biological Properties ◽  
Protecting Group ◽  
Dna And Rna ◽  
Double Stranded Dna ◽  
Physico Chemical ◽  
Rna Oligomers ◽  
Phosphate Linkage ◽  
Sirna Molecule ◽  
Fetal Bovine ◽  
The Stability

The condensation of the 5′-O-DMT-3′-deoxy-3′-aminothymidine with 3′-O-TBDMS-thymidine- 5′-aldehyde, followed by reduction of the resultant imine derivative and removal of tert-butyldimethylsilyl (TBDMS) protecting group, provided a dimer (denoted as TNHT), which is a congener of dithymidine phosphate with the phosphate linkage 3′-O-P(O)(OH)-O-5′ replaced with an amino group (–NH–). After phosphitylation of the 3′-OH group, the dimer TNHT was introduced (by the standard phosphoramidite approach) into a central part of the nonadecathymidylate. This oligomer exhibited lower affinity to the complementary single and double stranded DNA complements as compared to unmodified T19 oligonucleotide. The cleavage of modified oligomer with the snake venom and calf spleen phosphodiesterases was completely suppressed at the site of modification. RNA oligomers containing the TNHT dimer were used for preparation of siRNA molecules directed towards mRNA of BACE1 (beta-site amyloid precursor protein cleaving enzyme). The presence of the TNHT units at the 3′-ends of the RNA strands of the siRNA molecule (the siRNA itself is an effective gene expression inhibitor for BACE1) preserved the gene silencing activity and improved the stability of the modified siRNA in 10% fetal bovine serum.

scholarly journals Mapping three guanine oxidation products along DNA following exposure to three types of reactive oxygen species

2018 ◽  
Vol 121 ◽  
pp. 180-189 ◽  
Author(s):  
Brock Matter ◽  
Christopher L. Seiler ◽  
Kristopher Murphy ◽  
Xun Ming ◽  
Jianwei Zhao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxidation Products ◽  
Oxygen Species ◽  
Guanine Oxidation

scholarly journals In Vivo Bypass Efficiencies and Mutational Signatures of the Guanine Oxidation Products 2-Aminoimidazolone and 5-Guanidino-4-nitroimidazole

2004 ◽  
Vol 279 (42) ◽  
pp. 43568-43573 ◽  
Author(s):  
William L. Neeley ◽  
James C. Delaney ◽  
Paul T. Henderson ◽  
John M. Essigmann
Keyword(s):  
Oxidation Products ◽  
Mutational Signatures ◽  
Guanine Oxidation

Chemical synthesis of 13C-labelled monomers for the solid-phase and template controlled enzymatic synthesis of DNA and RNA oligomers

1994 ◽  
Vol 35 (36) ◽  
pp. 6649-6651 ◽  
Author(s):  
S. Quant ◽  
R.W. Wechselberger ◽  
M.A. Wolter ◽  
K.-H. Wörner ◽  
P. Schell ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Enzymatic Synthesis ◽  
Solid Phase ◽  
Dna And Rna ◽  
Rna Oligomers
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