Overproduction of α-Farnesene in Saccharomyces cerevisiae by Farnesene Synthase Screening and Metabolic Engineering

2021 ◽  
Vol 69 (10) ◽  
pp. 3103-3113
Author(s):  
Junhua Wang ◽  
Wei Jiang ◽  
Chaojuan Liang ◽  
Linghuan Zhu ◽  
Youran Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering

Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis

2021 ◽  
Author(s):  
Wenqi Shi ◽  
Jie Li ◽  
Yanfang Chen ◽  
Xiaohang Liu ◽  
Yefu Chen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Ethyl Acetate

scholarly journals Metabolic engineering of Saccharomyces cerevisiae for enhanced production of caffeic acid

2021 ◽  
Author(s):  
Pingping Zhou ◽  
Chunlei Yue ◽  
Bin Shen ◽  
Yi Du ◽  
Nannan Xu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Caffeic Acid ◽  
Enhanced Production

Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

2019 ◽  
Vol 21 (7) ◽  
pp. 1795-1808 ◽  
Author(s):  
Gregory Guirimand ◽  
Kentaro Inokuma ◽  
Takahiro Bamba ◽  
Mami Matsuda ◽  
Kenta Morita ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Cell Surface ◽  
Surface Display ◽  
Woody Biomass ◽  
Cell Surface Display ◽  
Xylitol Production ◽  
Display Technology ◽  
Pharmaceutical Industries

Xylitol is a major commodity chemical widely used in both the food and pharmaceutical industries.

scholarly journals Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiao-Jing Guo ◽  
Wen-Hai Xiao ◽  
Ying Wang ◽  
Ming-Dong Yao ◽  
Bo-Xuan Zeng ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering

scholarly journals Yeast-Based Biosynthesis of Natural Products From Xylose

2021 ◽  
Vol 9 ◽  
Author(s):  
Jian Zha ◽  
Miaomiao Yuwen ◽  
Weidong Qian ◽  
Xia Wu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Natural Products ◽  
Metabolic Engineering ◽  
State Of The Art ◽  
Commercial Production ◽  
Scheffersomyces Stipitis ◽  
Lignocellulosic Hydrolysates ◽  
Yeast Strains ◽  
Future Challenges ◽  
Biomass Materials

Xylose is the second most abundant sugar in lignocellulosic hydrolysates. Transformation of xylose into valuable chemicals, such as plant natural products, is a feasible and sustainable route to industrializing biorefinery of biomass materials. Yeast strains, including Saccharomyces cerevisiae, Scheffersomyces stipitis, and Yarrowia lipolytica, display some paramount advantages in expressing heterologous enzymes and pathways from various sources and have been engineered extensively to produce natural products. In this review, we summarize the advances in the development of metabolically engineered yeasts to produce natural products from xylose, including aromatics, terpenoids, and flavonoids. The state-of-the-art metabolic engineering strategies and representative examples are reviewed. Future challenges and perspectives are also discussed on yeast engineering for commercial production of natural products using xylose as feedstocks.

scholarly journals Metabolic engineering of <italic>Saccharomyces cerevisiae</italic> chassis

2020 ◽  
Vol 66 (3) ◽  
pp. 310-318
Author(s):  
Huan Lu ◽  
Yunfeng Zhang ◽  
Dan He ◽  
Xiaozhou Luo ◽  
Jiandong Huang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering

scholarly journals Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

2007 ◽  
Vol 73 (17) ◽  
pp. 5471-5476 ◽  
Author(s):  
Mervi H. Toivari ◽  
Laura Ruohonen ◽  
Andrei N. Miasnikov ◽  
Peter Richard ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Growth Medium ◽  
Fold Increase ◽  
Pichia Stipitis ◽  
Xylitol Dehydrogenase ◽  
Sugar Alcohols ◽  
Pentose Sugar ◽  
Encoding Gene ◽  
Pentose Sugars

ABSTRACT Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar d-ribose from d-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated d-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (d-ribose, d-ribulose, and d-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

Sign in / Sign up

Export Citation Format

Share Document