Novel Dual-Functional Enzyme Lip10 Catalyzes Lipase and Acyltransferase Activities in the Oleaginous Fungus Mucor circinelloides

2019 ◽  
Vol 67 (47) ◽  
pp. 13176-13184
Author(s):  
Xinyi Zan ◽  
Fengjie Cui ◽  
Jianing Sun ◽  
Shuai Zhou ◽  
Yuanda Song
Keyword(s):  
Mucor Circinelloides ◽  
Dual Functional ◽  
Oleaginous Fungus

13 C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides

2015 ◽  
Vol 197 ◽  
pp. 23-29 ◽  
Author(s):  
Lina Zhao ◽  
Huaiyuan Zhang ◽  
Liping Wang ◽  
Haiqin Chen ◽  
Yong Q. Chen ◽  
...  
Keyword(s):  
Metabolic Flux Analysis ◽  
Lipid Accumulation ◽  
Metabolic Flux ◽  
Mucor Circinelloides ◽  
Flux Analysis ◽  
Oleaginous Fungus

scholarly journals Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiuwen Wang ◽  
Hassan Mohamed ◽  
Yonghong Bao ◽  
Chen Wu ◽  
Wenyue Shi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Accumulation ◽  
Carbon Sources ◽  
Reducing Power ◽  
Acid Synthesis ◽  
Mucor Circinelloides ◽  
Microbial Lipids ◽  
Intracellular Lipid ◽  
Engineering Technology ◽  
Oleaginous Fungus

The fungus, Mucor lusitanicus, is of great interest for microbial lipids, because of its ability to accumulate intracellular lipid using various carbon sources. The biosynthesis of fatty acid requires the reducing power NADPH, and acetyl-CoA, which is produced by the cleavage of citrate in cytosol. In this study, we employed different strategies to increase lipid accumulation in the low lipid-producing fungi via metabolic engineering technology. Hence, we constructed the engineered strain of M. lusitanicus CBS 277.49 by using malate transporter (mt) and 2-oxoglutarate: malate antiporter (sodit) from M. circinelloides WJ11. In comparison with the control strain, the lipid content of the overexpressed strains of mt and sodit genes were increased by 24.6 and 33.8%, respectively. These results showed that mt and sodit can affect the distribution of malate in mitochondria and cytosol, provide the substrates for the synthesis of citrate in the mitochondria, and accelerate the transfer of citrate from mitochondria to cytosol, which could play a significant regulatory role in fatty acid synthesis leading to lipids over accumulation.

Role of malate transporter in lipid accumulation of oleaginous fungus Mucor circinelloides

2015 ◽  
Vol 100 (3) ◽  
pp. 1297-1305 ◽  
Author(s):  
Lina Zhao ◽  
José T. Cánovas-Márquez ◽  
Xin Tang ◽  
Haiqin Chen ◽  
Yong Q. Chen ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Mucor Circinelloides ◽  
Oleaginous Fungus ◽  
Malate Transporter

Value-added oil and animal feed production from corn-ethanol stillage using the oleaginous fungus Mucor circinelloides

2012 ◽  
Vol 107 ◽  
pp. 368-375 ◽  
Author(s):  
Debjani Mitra ◽  
Mary L. Rasmussen ◽  
Priyanka Chand ◽  
Venkat Reddy Chintareddy ◽  
Linxing Yao ◽  
...  
Keyword(s):  
Animal Feed ◽  
Value Added ◽  
Mucor Circinelloides ◽  
Corn Ethanol ◽  
Feed Production ◽  
Oleaginous Fungus

scholarly journals Genetic Modification of Mucor circinelloides to Construct Stearidonic Acid Producing Cell Factory

2019 ◽  
Vol 20 (7) ◽  
pp. 1683 ◽  
Author(s):  
Md. Khan ◽  
Junhuan Yang ◽  
Syed Hussain ◽  
Huaiyuan Zhang ◽  
Victoriano Garre ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Current Knowledge ◽  
Stearidonic Acid ◽  
Mucor Circinelloides ◽  
Cell Factory ◽  
Alpha Linolenic Acid ◽  
Homologous Overexpression ◽  
High Lipid ◽  
Oleaginous Fungus ◽  
Delta 6 Desaturase

Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of its nutritional value and pharmaceutical applications. Mucor circinelloides is a GLA producing filamentous fungus, which can be a useful tool to produce SDA. This study has, therefore, overexpressed the delta-15 desaturase (D15D) gene from Mortierella alpina in this fungus to construct a SDA-producing cell factory. To produce SDA in M. circinelloides, the homologous overexpression of D15D gene was analyzed. When the gene was overexpressed in M. circinelloides CBS 277.49, up to 5.0% SDA was accumulated in this strain. According to current knowledge, this is the first study describing the construction of a SDA-producing cell factory by overexpression of D15D gene in oleaginous fungus M. circinelloides. A new scope for further research has been established by this work to improve SDA production in this fungus, specifically in its high lipid-producing strain, WJ11.

scholarly journals Transcriptome Analysis of Oleaginous Fungus Mucor circinelloides WJ11 in Response to Exogenous Soybean Oil as Carbon Source

2021 ◽  
Vol 16 (6) ◽  
pp. 1934578X2110233
Author(s):  
Caili Sun ◽  
Aabid Manzoor Shah ◽  
Junhuan Yang ◽  
Zongmin Wang ◽  
Lanlan Zhu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Soybean Oil ◽  
Lipid Content ◽  
Metabolic Pathways ◽  
Mucor Circinelloides ◽  
Lipid Biosynthesis ◽  
Rna Seq ◽  
Plant Oil ◽  
Carbon Substrates ◽  
Oleaginous Fungus

Mucor circinelloides is an oleaginous fungus that utilizes a wide variety of carbon substrates for its growth. The different sources of carbon strongly influence the total lipid content of the fungus. These different carbon substrates are assimilated and dissimilated through different metabolic pathways before entering into the TAG synthesis pathway. In the present study, we attempted to explore the mechanism of ex-novo lipid biosynthesis in M. circinelloides WJ11 in response to exogenous plant oil as a carbon source through transcriptomic analysis. The lipid content of WJ11 grown in a media containing mixed soybean oil with glucose as a carbon source was up to 43.8%, an increase of 13.9% as compared to glucose alone as the carbon source. RNA-Seq analysis was performed to investigate global gene expression patterns in the oil-treated WJ11. Based on RNA-seq analysis, among the 4646 differentially expressed genes (DEGs), 2379 were up-regulated and 2267 down-regulated. The expression of acetyl-CoA synthetase, 6-phosphofructokinase, alcohol dehydrogenase (NADP+), fructose-bisphosphate aldolase, and pyruvate kinase was down-regulated while genes related to triglyceride synthesis were up-regulated. The majority of genes and pathways related to lipid biosynthesis were up-regulated indicating a diversion of metabolic pathways towards lipid biosynthesis. The data generated advance the genomic resources and provide insights into the mechanisms of ex-novo lipid accumulation in fungi that use exogenous oil as a carbon source.

Malic enzyme activity is not the only bottleneck for lipid accumulation in the oleaginous fungus Mucor circinelloides

2012 ◽  
Vol 97 (7) ◽  
pp. 3063-3072 ◽  
Author(s):  
Rosa Amarilis Rodríguez-Frómeta ◽  
Adrián Gutiérrez ◽  
Santiago Torres-Martínez ◽  
Victoriano Garre
Keyword(s):  
Enzyme Activity ◽  
Lipid Accumulation ◽  
Malic Enzyme ◽  
Mucor Circinelloides ◽  
Oleaginous Fungus ◽  
Malic Enzyme Activity

scholarly journals Microbial oil production from solid-state fermentation by a newly isolated oleaginous fungus, Mucor circinelloides Q531 from mulberry branches

2018 ◽  
Vol 5 (11) ◽  
pp. 180551 ◽  
Author(s):  
Weichuan Qiao ◽  
Junqi Tao ◽  
Yang Luo ◽  
Tianhao Tang ◽  
Jiahui Miao ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Cellulase Activity ◽  
Mucor Circinelloides ◽  
Chromatography Analysis ◽  
Gas Chromatography Analysis ◽  
Oleaginous Fungus ◽  
Main Components

In this study, a newly isolated oleaginous fungus, Mucor circinelloides ( M. circinelloides ) Q531, was able to convert mulberry branches into lipids. The highest yield and the maximum lipid content produced by the fungal cells were 42.43 ± 4.01 mg per gram dry substrate (gds) and 28.8 ± 2.85%, respectively. The main components of lignocellulosic biomass were gradually reduced during solid-state fermentation (SSF). Cellulose, hemicellulose and lignin were decreased from 45.11, 31.39 and 17.36% to 41.48, 28.71, and 15.1%, respectively. Gas chromatography analysis showed that the major compositions of the fermented products were palmitic acid (C16:0, 18.42%), palmitoleic acid (C16:1, 5.56%), stearic acid (C18:0, 5.87%), oleic acid (C18:1, 33.89%), linoleic acid (C18:2, 14.45%) and γ-linolenic acid (C18:3 n6, 22.53%) after 2 days of SSF. The fatty acid methyl esters contained unsaturated fatty acids with a ratio of 75.95%. The composition and content obtained in this study are more advantageous than those of many other biomass lipids. Meanwhile, the oleaginous fungus had a high cellulase activity of 1.39 ± 0.09 FPU gds −1 . The results indicate that the enzyme activity of the isolated fungus was capable of converting the cellulose and hemicelluloses to available sugar monomers which are beneficial for the production of lipids.

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