Mechanism Behind the Apparent Large Stokes Shift in LSSmOrange Investigated by Time-Resolved Spectroscopy

2015 ◽  
Vol 119 (47) ◽  
pp. 14880-14891 ◽  
Author(s):  
Eduard Fron ◽  
Herlinde De Keersmaecker ◽  
Susana Rocha ◽  
Yannick Baeten ◽  
Gang Lu ◽  
...  
Keyword(s):  
Stokes Shift ◽  
Time Resolved ◽  
Time Resolved Spectroscopy ◽  
Large Stokes Shift

scholarly journals Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mateusz Mieczkowski ◽  
Christian Steinmetzger ◽  
Irene Bessi ◽  
Ann-Kathrin Lenz ◽  
Alexander Schmiedel ◽  
...  
Keyword(s):  
Proton Transfer ◽  
Base Pair ◽  
Fluorescent Proteins ◽  
Stokes Shift ◽  
Fluorescence Emission ◽  
Rna Aptamers ◽  
Rna Aptamer ◽  
Time Resolved ◽  
Large Stokes Shift ◽  
Mechanistic Basis

AbstractFluorogenic RNA aptamers are synthetic functional RNAs that specifically bind and activate conditional fluorophores. The Chili RNA aptamer mimics large Stokes shift fluorescent proteins and exhibits high affinity for 3,5-dimethoxy-4-hydroxybenzylidene imidazolone (DMHBI) derivatives to elicit green or red fluorescence emission. Here, we elucidate the structural and mechanistic basis of fluorescence activation by crystallography and time-resolved optical spectroscopy. Two co-crystal structures of the Chili RNA with positively charged DMHBO+ and DMHBI+ ligands revealed a G-quadruplex and a trans-sugar-sugar edge G:G base pair that immobilize the ligand by π-π stacking. A Watson-Crick G:C base pair in the fluorophore binding site establishes a short hydrogen bond between the N7 of guanine and the phenolic OH of the ligand. Ultrafast excited state proton transfer (ESPT) from the neutral chromophore to the RNA was found with a time constant of 130 fs and revealed the mode of action of the large Stokes shift fluorogenic RNA aptamer.

Pressure effects on the crystal field and luminescence Stokes shift of Mn2+ (Td) in [(CH3)4N]2MnX4 (X: Cl, Br) by time-resolved spectroscopy

2009 ◽  
Vol 29 (2) ◽  
pp. 230-234 ◽  
Author(s):  
Y. Rodríguez-Lazcano ◽  
Lucie Nataf ◽  
Fernando Rodríguez
Keyword(s):  
Crystal Field ◽  
Stokes Shift ◽  
Pressure Effects ◽  
Time Resolved ◽  
Time Resolved Spectroscopy

A sensitive bio-probe for tracking lipid droplets with large Stokes shift and its application in cell imaging

2021 ◽  
pp. 119988
Author(s):  
Yizhe Yan ◽  
Shaoqing Li ◽  
Zhi-Hao Zhang ◽  
Jianbo Qu ◽  
Jian-Yong Wang
Keyword(s):  
Lipid Droplets ◽  
Cell Imaging ◽  
Stokes Shift ◽  
Large Stokes Shift

A novel near-infrared fluorescent probe based on isophorone for the bioassay of endogenous cysteine

2021 ◽  
Author(s):  
Hong-Bo Liu ◽  
Hai Xu ◽  
Xin Guo ◽  
Jian Xiao ◽  
Zheng-Hong Cai ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Rapid Identification ◽  
Large Stokes Shift

A near-infrared (NIR) fluorescent probe with a large Stokes shift (143 nm) for the rapid identification of Cys over Hcy and GSH in aqueous solution was developed.

scholarly journals Photophysical properties and fluorosolvatochromism of D–π–A thiophene based derivatives

RSC Advances ◽  
2020 ◽  
Vol 10 (71) ◽  
pp. 43459-43471
Author(s):  
Hussain A. Z. Sabek ◽  
Ahmed M. M. Alazaly ◽  
Dina Salah ◽  
Hesham S. Abdel-Samad ◽  
Mohamed A. Ismail ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Time Resolved ◽  
Time Resolved Spectroscopy ◽  
Solvation Models

Solvation-dependent photophysical properties of two push–pull thiophene-based compounds with donor–π–acceptor (D–π–A) structures were investigated using absorption, fluorescence emission and time resolved spectroscopy, and supported by different solvation models.

Sign in / Sign up

Export Citation Format

Share Document