ZIF-67 as a Template Generating and Tuning “Raisin Pudding”-Type Nanozymes with Multiple Enzyme-like Activities: Toward Online Electrochemical Detection of 3,4-Dihydroxyphenylacetic Acid in Living Brains

2020 ◽  
Author(s):  
Jia Liu ◽  
Wang Zhang ◽  
Meihong Peng ◽  
Guoyuan Ren ◽  
Lihao Guan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Dihydroxyphenylacetic Acid

Simultaneous determination of four monoamine metabolites and serotonin in cerebrospinal fluid by "high-performance" liquid chromatography with electrochemical detection; application for patients with Alzheimer's disease.

1988 ◽  
Vol 34 (4) ◽  
pp. 680-684 ◽  
Author(s):  
E Koyama ◽  
A Minegishi ◽  
T Ishizaki
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Detection System ◽  
Dihydroxyphenylacetic Acid ◽  
Assay Procedure ◽  
Lumbar Cerebrospinal Fluid

Abstract We describe an optimized, sensitive assay procedure for simultaneously determining 3-methoxy-4-hydroxyphenylglycol, 3,4-dihydroxyphenylacetic acid, and serotonin in human lumbar cerebrospinal fluid. The assay is based on liquid chromatography with an electrochemical detection system equipped with a newly developed, highly sensitive graphite electrode. Before assay, samples are deproteinized with perchloric acid, 0.2 mol/L. The within- and between-day CVs were less than 2.5% and 6.7%, respectively, for all compounds. The lower limits of detection for 3-methoxy-4-hydroxyphenylglycol and 3,4-dihydroxyphenylacetic acid were 0.5 pg and for the other three analytes 1.5 pg per injection. Preliminary data on monoaminergic metabolites by the current assay showed that concentrations of dopaminergic and serotonergic metabolites were significantly (P less than 0.05) lower in patients with Alzheimer's disease than in the age-matched controls. In addition, the concentration of 3,4-dihydroxyphenylacetic acid correlated significantly (P less than 0.01) with that of homovanillic acid, suggesting that monitoring either of these metabolites in cerebrospinal fluid can be used as an index of central dopaminergic activity.

Sign in / Sign up

Export Citation Format

Share Document