Quantitative Single-Particle Fluorescence Imaging Elucidates Semiconductor Shell Influence on Ag@TiO2 Photocatalysis

2021 ◽  
Vol 13 (6) ◽  
pp. 7680-7687
Author(s):  
Yujie Liu ◽  
Kun Zhang ◽  
Xiaochaoran Tian ◽  
Lei Zhou ◽  
Jianwei Liu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Particle ◽  
Tio2 Photocatalysis

Progress on the labeling and single-particle tracking technologies of viruses

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3336-3346 ◽  
Author(s):  
Li-Li Huang ◽  
Hai-Yan Xie
Keyword(s):  
Fluorescence Imaging ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Imaging Technologies ◽  
Recent Advances

We review recent advances in virus labeling and the emerging fluorescence imaging technologies used in the imaging and tracking of viruses.

Pore selectivity and electron transfers in HZSM-5 single crystals: a Raman microspectroscopy mapping and confocal fluorescence imaging combined study

2020 ◽  
Vol 22 (22) ◽  
pp. 12745-12756
Author(s):  
A. Moissette ◽  
M. Hureau ◽  
M. Moreau ◽  
J. P. Cornard
Keyword(s):  
Single Crystals ◽  
Fluorescence Imaging ◽  
Single Particle ◽  
Raman Microspectroscopy ◽  
Single Particle Level ◽  
Confocal Fluorescence ◽  
Particle Level ◽  
Confocal Fluorescence Imaging ◽  
Electron Transfers

Electron transfers at the single particle level in HZSM-5 zeolite are followed by combining Raman microspectroscopy mapping and confocal fluorescence imaging. The effects of pore accessibility and guest diffusion on reactivity are investigated.

scholarly journals Detecting and Quantifying Colocalization of Cell Surface Molecules by Single Particle Fluorescence Imaging

2003 ◽  
Vol 85 (6) ◽  
pp. 4110-4121 ◽  
Author(s):  
Ian E.G. Morrison ◽  
Ioannis Karakikes ◽  
Rosamund E. Barber ◽  
Nelson Fernández ◽  
Richard J. Cherry
Keyword(s):  
Cell Surface ◽  
Fluorescence Imaging ◽  
Single Particle ◽  
Cell Surface Molecules ◽  
Surface Molecules

scholarly journals Detection of Dimers of Dimers of Human Leukocyte Antigen (HLA)–DR on the Surface of Living Cells by Single-Particle Fluorescence Imaging

1998 ◽  
Vol 140 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Richard J. Cherry ◽  
Keith M. Wilson ◽  
Kathy Triantafilou ◽  
Peter O'Toole ◽  
Ian E.G. Morrison ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Fluorescence Imaging ◽  
Mhc Class Ii ◽  
Single Particle ◽  
Human Leukocyte ◽  
Living Cells ◽  
Solid Substrate ◽  
Oligomeric State ◽  
Leukocyte Antigen ◽  
Hla Dr

The technique of single-particle fluorescence imaging was used to investigate the oligomeric state of MHC class II molecules on the surface of living cells. Cells transfected with human leukocyte antigen (HLA)–DR A and B genes were labeled at saturation with a univalent probe consisting of Fab coupled to R-phycoerythrin. Analysis of the intensities of fluorescent spots on the cell surface revealed the presence of single and double particles consistent with the simultaneous presence of HLA-DR heterodimers and dimers of dimers. The proportion of double particles was lower at 37°C than at 22°C, suggesting that the heterodimers and dimers of dimers exist in a temperature-dependent equilibrium. These results are discussed in the context of a possible role for HLA-DR dimers of dimers in T cell receptor–MHC interactions. The technique is validated by demonstrating that fluorescence imaging can distinguish between dimers and tetramers of human erythrocyte spectrin deposited from solution onto a solid substrate. The methodology will have broad applicability to investigation of the oligomeric state of immunological and other membrane-bound receptors in living cells.

scholarly journals Enhancing the photostability of poly(phenylene ethynylene) for single particle studies

2017 ◽  
Vol 16 (12) ◽  
pp. 1821-1831 ◽  
Author(s):  
C. F. Calver ◽  
B. A. Lago ◽  
K. S. Schanze ◽  
G. Cosa
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Particle ◽  
Phenylene Ethynylene ◽  
Conjugated Polyelectrolytes ◽  
Imaging Studies ◽  
Single Molecule Fluorescence ◽  
Enhanced Photostability ◽  
The Way

Enhanced photostability of conjugated polyelectrolytes achieved by using anti-fading agents opens the way for advanced single molecule fluorescence imaging studies.

Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging

2003 ◽  
Vol 31 (6) ◽  
pp. 1453-1455 ◽  
Author(s):  
I. Karakikes ◽  
R.E. Barber ◽  
I.E.G. Morrison ◽  
N. Fernández ◽  
R.J. Cherry
Keyword(s):  
Cell Surface ◽  
Fluorescence Imaging ◽  
Mhc Class Ii ◽  
Spatial Resolution ◽  
Single Particle ◽  
High Spatial Resolution ◽  
Chromatic Aberration ◽  
Class Ii ◽  
Dual Wavelength ◽  
Surface Receptors

Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.

Brightness calibrates particle size in single particle fluorescence imaging

2015 ◽  
Vol 40 (7) ◽  
pp. 1242 ◽  
Author(s):  
Zhihe Liu ◽  
Zezhou Sun ◽  
Weihua Di ◽  
Weiping Qin ◽  
Zhen Yuan ◽  
...  
Keyword(s):  
Particle Size ◽  
Fluorescence Imaging ◽  
Single Particle
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