scholarly journals 3D-Printed, Modular, and Parallelized Microfluidic System with Customizable Scaffold Integration to Investigate the Roles of Basement Membrane Topography on Endothelial Cells

2021 ◽  
Author(s):  
Curtis G. Jones ◽  
Tianjiao Huang ◽  
Jay H. Chung ◽  
Chengpeng Chen
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Microfluidic System ◽  
Membrane Topography ◽  
3D Printed

scholarly journals A 3D-Printed, Modular, and Parallelized Microfluidic System with Customizable ECM Integration to Investigate the Roles of Basement Membrane Topography on Endothelial Cells

2020 ◽  
Author(s):  
Curtis G. Jones ◽  
Tianjiao Huang ◽  
Jay H. Chung ◽  
Chengpeng Chen
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
3D Structure ◽  
Microfluidic System ◽  
Fully Integrated ◽  
Membrane Topography ◽  
3D Printed ◽  
Easy Integration

<p>Because dysfunctions of endothelial cells are involved in many pathologies, <i>in vitro </i>endothelial cell models for pathophysiological and pharmaceutical studies have been a valuable research tool. Although numerous microfluidic-based endothelial models have been reported, they had the cells cultured on a flat surface without considering the possible 3D structure of the native ECM. Endothelial cells rest on the basement membrane <i>in vivo</i>, which contains an aligned microfibrous topography. To better understand and model the cells, it is necessary to know if and how the fibrous topography can affect endothelial functions. With conventional fully integrated microfluidic apparatus, it is difficult to include additional topographies in a microchannel. Therefore, we developed a modular microfluidic system by 3D-printing and electrospinning, which enabled easy integration and switching of desired ECM topographies. Also, with standardized designs, the system allowed for high flow rates up to 4000 µL/min, which covered the full shear stress range for endothelial studies. We found that the aligned fibrous topography on the ECM altered arginine metabolism in endothelial cells, and thus increased nitric oxide production. To the best of our knowledge, this is the most versatile endothelial model that has been reported, and the new knowledge generated thereby lays a groundwork for future endothelial research and modeling. </p>

scholarly journals A 3D-Printed, Modular, and Parallelized Microfluidic System with Customizable ECM Integration to Investigate the Roles of Basement Membrane Topography on Endothelial Cells

2020 ◽  
Author(s):  
Curtis G. Jones ◽  
Tianjiao Huang ◽  
Jay H. Chung ◽  
Chengpeng Chen
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
3D Structure ◽  
Microfluidic System ◽  
Fully Integrated ◽  
Membrane Topography ◽  
3D Printed ◽  
Easy Integration

<p>Because dysfunctions of endothelial cells are involved in many pathologies, <i>in vitro </i>endothelial cell models for pathophysiological and pharmaceutical studies have been a valuable research tool. Although numerous microfluidic-based endothelial models have been reported, they had the cells cultured on a flat surface without considering the possible 3D structure of the native ECM. Endothelial cells rest on the basement membrane <i>in vivo</i>, which contains an aligned microfibrous topography. To better understand and model the cells, it is necessary to know if and how the fibrous topography can affect endothelial functions. With conventional fully integrated microfluidic apparatus, it is difficult to include additional topographies in a microchannel. Therefore, we developed a modular microfluidic system by 3D-printing and electrospinning, which enabled easy integration and switching of desired ECM topographies. Also, with standardized designs, the system allowed for high flow rates up to 4000 µL/min, which covered the full shear stress range for endothelial studies. We found that the aligned fibrous topography on the ECM altered arginine metabolism in endothelial cells, and thus increased nitric oxide production. To the best of our knowledge, this is the most versatile endothelial model that has been reported, and the new knowledge generated thereby lays a groundwork for future endothelial research and modeling. </p>

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

A 3D-printed Microfluidic System for Automated and Near Real-time Quantitation of Biofilm-induced indole

2019 ◽  
Author(s):  
Giraso Kabandana ◽  
Curtis G. Jones ◽  
Sahra Khan Sharifi ◽  
Chengpeng Chen
Keyword(s):  
Real Time ◽  
Release Kinetics ◽  
Microfluidic System ◽  
3D Printed

We developed a novel microfluidic system that enables automated and near real-time quantitation of indole release kinetics from biofilms.

scholarly journals Ultra-Portable Smartphone Controlled Integrated Digital Microfluidic System in a 3D-Printed Modular Assembly

Micromachines ◽  
2015 ◽  
Vol 6 (9) ◽  
pp. 1289-1305 ◽  
Author(s):  
Mohamed Yafia ◽  
Ali Ahmadi ◽  
Mina Hoorfar ◽  
Homayoun Najjaran
Keyword(s):  
Microfluidic System ◽  
Modular Assembly ◽  
3D Printed ◽  
Digital Microfluidic

Prolonged culture of endothelial cells and deposition of basement membrane modify the recruitment of neutrophils

2005 ◽  
Vol 310 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Lynn M. Butler ◽  
G. Ed Rainger ◽  
Mahbub Rahman ◽  
Gerard B. Nash
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Prolonged Culture

Matrigel induces thymosin beta 4 gene in differentiating endothelial cells

1995 ◽  
Vol 108 (12) ◽  
pp. 3685-3694 ◽  
Author(s):  
D.S. Grant ◽  
J.L. Kinsella ◽  
M.C. Kibbey ◽  
S. LaFlamme ◽  
P.D. Burbelo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Morphological Differentiation ◽  
Tube Formation ◽  
Vessel Formation ◽  
Thymosin Beta 4 ◽  
Cdna Hybridization ◽  
Matrix Components ◽  
Cells Cultured

We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes. The mRNA for one clone, thymosin beta 4, was increased 5-fold. Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues. Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel. An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel. The results suggest that thymosin beta 4 is induced and likely involved in differentiating endothelial cells. Thymosin beta 4 may play a role in vessel formation in vivo.

scholarly journals Identification of a multifunctional, cell-binding peptide sequence from the a1(NC1) of type IV collagen.

1990 ◽  
Vol 111 (4) ◽  
pp. 1583-1591 ◽  
Author(s):  
E C Tsilibary ◽  
L A Reger ◽  
A M Vogel ◽  
G G Koliakos ◽  
S S Anderson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Type Iv Collagen ◽  
Solid Phase ◽  
Peptide Sequence ◽  
Cell Binding ◽  
Type Iv ◽  
Specific Manner ◽  
Dose Dependent ◽  
Nc1 Domain

We have previously identified three distinctive amino acid sequences from type IV collagen which specifically bound to heparin and also inhibited the binding of heparin to intact type IV collagen. One of these chemically synthesized domains, peptide Hep-I, has the sequence TAGSCLRKFSTM and originates from the a1(noncollagenous [NC1]) chain of type IV collagen (Koliakos, G. G., K. K. Koliakos, L. T. Furcht, L. A. Reger, and E. C. Tsilibary. 1989. J. Biol. Chem. 264:2313-2323). We describe in this report that this same peptide also bound to intact type IV collagen in solid-phase assays, in a dose-dependent and specific manner. Interactions between peptide Hep-I and type IV collagen in solution resulted in inhibition of the assembly process of this basement membrane glycoprotein. Therefore, peptide Hep-I should represent a major recognition site in type IV collagen when this protein polymerizes to form a network. In addition, solid phase-immobilized peptide Hep-I was able to promote the adhesion and spreading of bovine aortic endothelial cells. When present in solution, peptide Hep-I competed for the binding of these cells to type IV collagen- and NC1 domain-coated substrata in a dose-dependent manner. Furthermore, radiolabeled peptide Hep-I in solution also bound to endothelial cells in a dose-dependent and specific manner. The binding of radiolabeled Hep-I to endothelial cells could be inhibited by an excess of unlabeled peptide. Finally, in the presence of heparin or chondroitin/dermatan sulfate glycosaminoglycan side chains, the binding of endothelial cells to peptide Hep-I and NC1 domain-coated substrates was also inhibited. We conclude that peptide Hep-I should have a number of functions. The role of this type IV collagen-derived sequence in such diverse phenomena as self-association, heparin binding and cell binding and adhesion makes Hep-I a crucial domain involved in the determination of basement membrane ultrastructure and cellular interactions with type IV collagen-containing matrices.

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