Effect of 3D-Fibroblast Dermis Constructed by Layer-by-Layer Cell Coating Technique on Tight Junction Formation and Function in Full-Thickness Skin Equivalent

2021 ◽  
Author(s):  
Masato Murakami ◽  
Takami Akagi ◽  
Yumi Sasano ◽  
Mitsuru Akashi
Keyword(s):  
Tight Junction ◽  
Layer By Layer ◽  
Full Thickness ◽  
Coating Technique ◽  
Layer Cell ◽  
Tight Junction Formation ◽  
Thickness Skin ◽  
Junction Formation ◽  
Cell Coating ◽  
And Function

Observation of a tight junction structure generated in LbL‐3D Skin reconstructed by layer‐by‐layer cell coating technique

2021 ◽  
Author(s):  
Masato Murakami ◽  
Takami Akagi ◽  
Yumi Sasano ◽  
Tomohiro Chiba ◽  
Hirokazu Narita ◽  
...  
Keyword(s):  
Tight Junction ◽  
Layer By Layer ◽  
Coating Technique ◽  
Layer Cell ◽  
Cell Coating ◽  
Junction Structure ◽  
3D Skin

Layer-by-layer cell coating technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β-cell spheroids

Biomaterials ◽  
2018 ◽  
Vol 160 ◽  
pp. 82-91 ◽  
Author(s):  
Yasunari Fukuda ◽  
Takami Akagi ◽  
Tadafumi Asaoka ◽  
Hidetoshi Eguchi ◽  
Kazuki Sasaki ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Layer By Layer ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Coating Technique ◽  
Cell Spheroids ◽  
Layer Cell ◽  
Rapid Fabrication ◽  
Cell Coating ◽  
And Function

Three-dimensional idiopathic pulmonary fibrosis model using a layer-by-layer cell coating technique

2021 ◽  
Author(s):  
Hidenori Kuno ◽  
Takami Akagi ◽  
Eriko Fukui ◽  
Hiroyuki Yamato ◽  
Mitsuru Akashi ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Coating Technique ◽  
Layer Cell ◽  
Cell Coating

scholarly journals Na+/K+-ATPase regulates tight junction formation and function during mouse preimplantation development

2006 ◽  
Vol 289 (2) ◽  
pp. 406-419 ◽  
Author(s):  
Michelle I. Violette ◽  
Pavneesh Madan ◽  
Andrew J. Watson
Keyword(s):  
Tight Junction ◽  
Preimplantation Development ◽  
Tight Junction Formation ◽  
Junction Formation ◽  
And Function

scholarly journals Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice

2017 ◽  
Vol 23 (11) ◽  
pp. 771-785 ◽  
Author(s):  
Michele D Calder ◽  
Nicole A Edwards ◽  
Dean H Betts ◽  
Andrew J Watson
Keyword(s):  
Tight Junction ◽  
Blastocyst Development ◽  
Tight Junction Formation ◽  
Junction Formation ◽  
And Function

Construction of three-dimensional vascularized functional human liver tissue using a layer-by-layer cell coating technique

Biomaterials ◽  
2017 ◽  
Vol 133 ◽  
pp. 263-274 ◽  
Author(s):  
Kazuki Sasaki ◽  
Takami Akagi ◽  
Tadafumi Asaoka ◽  
Hidetoshi Eguchi ◽  
Yasunari Fukuda ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Human Liver Tissue ◽  
Coating Technique ◽  
Layer Cell ◽  
Cell Coating

scholarly journals Participation of plasma membrane proteins in the formation of tight junction by cultured epithelial cells

1983 ◽  
Vol 96 (3) ◽  
pp. 693-702 ◽  
Author(s):  
EB Griepp ◽  
WJ Dolan ◽  
ES Robbins ◽  
DD Sabatini
Keyword(s):  
Protein Synthesis ◽  
Steady State ◽  
Tight Junction ◽  
Messenger Rna ◽  
Freeze Fracture ◽  
Epithelial Cell Line ◽  
Experimental Conditions ◽  
Tight Junction Formation ◽  
Junction Formation ◽  
Spinner Culture

Measurements of the transepithelial electrical resistance correlated with freeze-fracture observations have been used to study the process of tight junction formation under various experimental conditions in monolayers of the canine kidney epithelial cell line MDCK. Cells derived from previously confluent cultures and plated immediately after trypsin- EDTA dissociation develop a resistance that reaches its maximum value of several hundred ohms-cm(2) after approximately 24 h and falls to a steady-state value of 80-150 ohms- cm(2) by 48 h. The rise in resistance and the development of tight junctions can be completely and reversibly prevented by the addition of 10 μg/ml cycloheximide at the time of plating, but not when this inhibitor is added more than 10 h after planting. Thus tight junction formation consists of separable synthetic and assembly phases. These two phases can also be dissociated and the requirement for protein synthesis after plating eliminated if, following trypsinization, the cells are maintained in spinner culture for 24 h before plating. The requirement for protein synthesis is restored, however, if cells maintained in spinner culture are treated with trypsin before plating. Actinomycin D prevents development of resistance only in monolayers formed from cells derived from sparse rather than confluent cultures, but new mRNA synthesis is not required if cells obtained from sparse cultures are maintained for 24 h in spinner culture before plating. Once a steady-state resistance has been reached, its maintenance does not require either mRNA or protein synthesis; in fact, inhibition of protein synthesis causes a rise in the resistance over a 30-h period. Following treatments that disrupt the junctions in steady- state monolayers recovery of resistance also does not require protein synthesis. These observations suggest that proteins are involved in tight junction formation. Such proteins, which do not turn over rapidly under steady-state conditions, are destroyed by trypsinization and can be resynthesized in the absence of stable cell-cell or cell-substratum contact. Messenger RNA coding for proteins involved in tight junction formation is stable except when cells are sparsely plated, and can also be synthesized without intercellular contacts or cell-substratum attachment.

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