scholarly journals Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

10.3791/62148 ◽  
2021 ◽  
Author(s):  
Jackson Coole ◽  
Alex Kortum ◽  
Yubo Tang ◽  
Imran Vohra ◽  
Yajur Maker ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Resource Limited Settings ◽  
Isothermal Nucleic Acid Amplification ◽  
Resource Limited

scholarly journals Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

2021 ◽  
Vol 9 ◽  
pp. S6
Author(s):  
Jackson Coole ◽  
Alex Kortum ◽  
Yubo Tang ◽  
Imran Vohra ◽  
Mary Natoli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Resource Limited Settings ◽  
Isothermal Nucleic Acid Amplification ◽  
Resource Limited

scholarly journals A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Sensors ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 23418-23430 ◽  
Author(s):  
Pascal Craw ◽  
Ruth Mackay ◽  
Angel Naveenathayalan ◽  
Chris Hudson ◽  
Manoharanehru Branavan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions

2021 ◽  
Author(s):  
Andrew H. Buultjens ◽  
Koen Vandelannoote ◽  
Liam K. Sharkey ◽  
Benjamin P. Howden ◽  
Ian R. Monk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Fluorescence Detection ◽  
High Performance ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern

2021 ◽  
Author(s):  
Yuanhao Liang ◽  
Hongqing Lin ◽  
Lirong Zou ◽  
Jianhui Zhao ◽  
Baisheng Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Resource Limited Settings ◽  
Nucleic Acid Amplification Tests ◽  
Specific Assay ◽  
Resource Limited ◽  
Allele Specific ◽  
New System

We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented for screening of SARS-CoV-2 variants in resource-limited settings. This approach can be adapted for emerging mutations and implemented in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests using existing resources and extracted nucleic acid.

Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification

2017 ◽  
Vol 9 (18) ◽  
pp. 15245-15253 ◽  
Author(s):  
Fan Li ◽  
Xiaoguo Liu ◽  
Bin Zhao ◽  
Juan Yan ◽  
Qian Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Real Time Monitoring ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

scholarly journals An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests

2020 ◽  
Vol 169 ◽  
pp. 112651 ◽  
Author(s):  
Mingdian Liu ◽  
Yuxin Zhao ◽  
Hosein Monshat ◽  
Zheyuan Tang ◽  
Zuowei Wu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Time Analysis ◽  
Nucleic Acid Amplification Tests ◽  
Isothermal Nucleic Acid Amplification ◽  
Real Time Analysis ◽  
Sensor Platform ◽  
Paper Sensor
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