scholarly journals A Local Allosteric Network in Heat Shock Protein 70 (Hsp70) Links Inhibitor Binding to Enzyme Activity and Distal Protein–Protein Interactions

2018 ◽  
Vol 13 (11) ◽  
pp. 3142-3152 ◽  
Author(s):  
Silvia Rinaldi ◽  
Victoria A. Assimon ◽  
Zapporah T. Young ◽  
Giulia Morra ◽  
Hao Shao ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 70 ◽  
Protein Protein Interactions ◽  
Inhibitor Binding

scholarly journals High-throughput screen for inhibitors of protein–protein interactions in a reconstituted heat shock protein 70 (Hsp70) complex

2018 ◽  
Vol 293 (11) ◽  
pp. 4014-4025 ◽  
Author(s):  
Isabelle R. Taylor ◽  
Bryan M. Dunyak ◽  
Tomoko Komiyama ◽  
Hao Shao ◽  
Xu Ran ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
High Throughput ◽  
Protein Interactions ◽  
Heat Shock Protein 70 ◽  
High Throughput Screen ◽  
Protein Protein Interactions

scholarly journals Two distinct classes of co-chaperones compete for the EEVD motif in heat shock protein 70 (Hsp70) to tune its activity

2021 ◽  
Author(s):  
Oleta T Johnson ◽  
Cory M Nadel ◽  
Emma C Carroll ◽  
Taylor Arhar ◽  
Jason E Gestwicki
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 70 ◽  
Protein Protein Interactions ◽  
Hsp70 Family ◽  
C Terminus ◽  
Class B ◽  
Chaperone Binding

Chaperones of the heat shock protein 70 (Hsp70) family engage in protein-protein interactions (PPIs) with many co-chaperones. One hotspot for co-chaperone binding is the EEVD motif that is found at the extreme C-terminus of cytoplasmic Hsp70s. This motif is known to bind tetratricopeptide repeat (TPR) domain co-chaperones, such as the E3 ubiquitin ligase CHIP, and Class B J-domain proteins (JDPs), such as DnaJB4. Although complexes between Hsp70-CHIP and Hsp70-DnaJB4 are both important for chaperone functions, the molecular determinants that dictate the competition between these co-chaperones are not clear. Using a collection of EEVD-derived peptides, we find that DnaJB4 binds to the IEEVD motif of Hsp70s, but not the related MEEVD motif of cytoplasmic Hsp90s. Then, we explored which residues are critical for binding to CHIP and DnaJB4, revealing that they rely on some shared features of the IEEVD motif, such as the C-terminal carboxylate. However, they also had unique preferences, especially at the isoleucine position. Finally, we observed a functionally important role for competition between CHIP and DnaJB4 in vitro, as DnaJB4 can limit the ubiquitination activity of the Hsp70-CHIP complex, while CHIP suppresses the chaperone activities of Hsp70-DnaJB4. Together, these results suggest that the EEVD motif has evolved to support diverse PPIs, such that competition between co-chaperones could help guide whether Hsp70-bound proteins are folded or degraded.

scholarly journals Exploration of Benzothiazole Rhodacyanines as Allosteric Inhibitors of Protein–Protein Interactions with Heat Shock Protein 70 (Hsp70)

2018 ◽  
Vol 61 (14) ◽  
pp. 6163-6177 ◽  
Author(s):  
Hao Shao ◽  
Xiaokai Li ◽  
Michael A. Moses ◽  
Luke A. Gilbert ◽  
Chakrapani Kalyanaraman ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 70 ◽  
Protein Protein Interactions ◽  
Allosteric Inhibitors

Influence of stock density on digestive enzyme activity (trypsin), heat shock protein 70 (HSP70), and oxidative stress biomarkers of narrow clawed crayfish, Astacus leptodactylus Eschscholtz, 1823 (Decapoda, Astacidae)

Crustaceana ◽  
2016 ◽  
Vol 89 (10) ◽  
pp. 1193-1202 ◽  
Author(s):  
Ö. Aksu ◽  
F. Kutluyer ◽  
E. Can ◽  
M. Eri̇şi̇r ◽  
F. Benzer
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Digestive Enzyme ◽  
Stocking Density ◽  
Astacus Leptodactylus ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers

Stocking density causes changes in behaviour and physiology of aquatic animals and high density can cause oxidative damage in living cells. Therefore, experiments were designed to investigate the effects of stocking density on the oxidant-antioxidant response (glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA)), digestive enzyme activity (trypsin), and heat shock protein 70 (HSP70) of narrow clawed crayfish (Astacus leptodactylus). The experiment was conducted to assess effects by stocking 3, 6, 9 and 12 crayfish/m2. Crayfish were stocked into 12 fiberglass tanks, three replicates per treatment and fed for 45 days. Our results indicated that activity of GSH-Px, MDA, and GSH levels increased in hepatopancreas () and muscle tissues () with increasing stock concentration while CAT activity decreased (). Trypsin and HSP70 insignificantly increased in haemolymph. In conclusion, oxidative stress biomarkers were negatively influenced by increased quantitative changes in stocking density.

scholarly journals Heat Shock Protein 90 Mediates Protein-protein Interactions between Human Aminoacyl-tRNA Synthetases

2000 ◽  
Vol 275 (41) ◽  
pp. 31682-31688 ◽  
Author(s):  
Jeongwoo Kang ◽  
Taeho Kim ◽  
Young-Gyu Ko ◽  
Seung Bae Rho ◽  
Sang Gyu Park ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 90 ◽  
Protein Protein Interactions ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases

scholarly journals Computational analyses of amino acid molecules of heat shock protein-70 for elucidating its evolutionary diversity and protein interactions in selected farm animals

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
A. B. Sikiru ◽  
O. J. Makinde ◽  
E. Opoola ◽  
S. K. Omotugba ◽  
A. R. Musa
Keyword(s):  
Amino Acid ◽  
Standard Deviation ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 70 ◽  
Protein Sequences ◽  
Farm Animals ◽  
Adaptation To Heat ◽  
Computational Analyses

Abstract Background The heat shock protein-70 (HSP70) is a protein associated with response and adaptation to stress, as well as protection of the cells against thermal and oxidative stress in animals. It is an evolutionarily conserved protein, but its expression has been reportedly varied. Therefore, this study implemented computational analyses of the amino acid sequences of this gene for a better understanding of the evolutionary and protein interactions variations associated with the gene to facilitate its exploitation for the breeding of animals with increasing adaptation to heat stress. Results The result showed that there is a wide evolutionary distance between humans and the selected farm animals studied but elegans shared a common evolutionary relationship with the farm animals. The sequence identity analysis returned exact matches among the sequences as minimum = 8.09%, maximum = 98.58%, and mean ± SD = 71.03 ± 26.3% across all the species, while the sequence similarities resemblance among the sequences were minimum = 16.49%, maximum = 100%, and mean ± SD = 78.99 ± 24.39%. The global block substitution matrix (BLOSUM62) analysis returned minimum = 0.18, maximum = 0.98, and mean ± SD = 0.62 ± 0.34. The analysis of the molecular weight of the protein sequences returned minimum = 5.70 kDa, maximum = 6.41 kDa, mean = 6.28 kDa, and standard deviation 0.17 kDa, and the isoelectric point of the protein sequences was minimum = 4.55, maximum = 7.17, mean = 5.56, and standard deviation = 0.65 while the hydrophobicity of the protein sequences were minimum = 45.20 kcal/mol, maximum = 53.02 kcal/mol, mean = 47.81 kcal/mol, and standard deviation = 1.85 kcal/mol. Conclusion The outcomes of the computational analyses led to the conclusion that variations exist in the conservations of amino acid residues of the gene in the studied farm and non-farm animals, and this is responsible for the differences and similarities in the expression of the HSP70 gene in different animals. It was also concluded that elegans are suitable model that could be exploited for a better understanding of response and adaptation to heat stress in duck, chicken, cattle, sheep, and goat when focusing on regulation and expression of heat shock protein gene 70 (HSP70).

scholarly journals Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70.hsp56) heterocomplex

1994 ◽  
Vol 269 (15) ◽  
pp. 11155-11161
Author(s):  
M.J. Czar ◽  
J.K. Owens-Grillo ◽  
K.D. Dittmar ◽  
K.A. Hutchison ◽  
A.M. Zacharek ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Protein Protein Interactions

scholarly journals The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment

2017 ◽  
Vol 42 (4) ◽  
pp. 279 ◽  
Author(s):  
R. Amizar ◽  
S. Suharti ◽  
J. Jakaria ◽  
R. Mutia
Keyword(s):  
Body Weight ◽  
Enzyme Activity ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Body Weight Gain ◽  
Tropical Environment ◽  
Air Conditioner ◽  
Feed Conversion ◽  
Organic Selenium

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P<0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment.

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