scholarly journals 1,2,3-Triazole Rings as a Disulfide Bond Mimetic in Chimeric AGRP-Melanocortin Peptides: Design, Synthesis, and Functional Characterization

2017 ◽  
Vol 9 (5) ◽  
pp. 1001-1013 ◽  
Author(s):  
Srinivasa R. Tala ◽  
Anamika Singh ◽  
Cody J. Lensing ◽  
Sathya M. Schnell ◽  
Katie T. Freeman ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Functional Characterization ◽  
Design Synthesis ◽  
Melanocortin Peptides

Functional characterization of the modified melanocortin peptides responsible for ligand selectivity at the human melanocortin receptors

Peptides ◽  
2006 ◽  
Vol 27 (11) ◽  
pp. 2836-2845 ◽  
Author(s):  
Min Chen ◽  
Keith E. Georgeson ◽  
Carroll M. Harmon ◽  
Carrie Haskell-Luevano ◽  
Yingkui Yang
Keyword(s):  
Functional Characterization ◽  
Melanocortin Receptors ◽  
Ligand Selectivity ◽  
Melanocortin Peptides

Estimating Optimized Stress Bounds in Early Stage Design of Compliant Mechanisms

2017 ◽  
Vol 139 (6) ◽  
Author(s):  
Sree Kalyan Patiballa ◽  
Girish Krishnan
Keyword(s):  
Compliant Mechanisms ◽  
Compliant Mechanism ◽  
Early Stage ◽  
Functional Characterization ◽  
Stress Constraints ◽  
Design Synthesis ◽  
Stage Design ◽  
Computationally Intensive ◽  
Early Stage Design ◽  
Selection Of

Design synthesis of distributed compliant mechanisms is often a two-stage process involving (a) conceptual topology synthesis and a subsequent (b) refinement stage to meet strength and manufacturing specifications. The usefulness of a solution is ascertained only after the sequential completion of these two steps that are, in general, computationally intensive. This paper presents a strategy to rapidly estimate final operating stresses even before the actual refinement process. This strategy is based on the uniform stress distribution metric, and a functional characterization of the different members that constitute the compliant mechanism topology. Furthermore, this paper uses the underlying mechanics of stress bound estimation to propose two rule of thumb guidelines for insightful selection of topologies and systematically modifying them for an application. The selection of the best conceptual solution in the early stage design avoids refinement of topologies that inherently may not meet the stress constraints. This paper presents two examples that illustrate these guidelines through the selection and refinement of topologies for a planar compliant gripper application.

scholarly journals Novel Inhibitors of Rad6 Ubiquitin Conjugating Enzyme: Design, Synthesis, Identification, and Functional Characterization

2013 ◽  
Vol 12 (4) ◽  
pp. 373-383 ◽  
Author(s):  
Matthew A. Sanders ◽  
Ghali Brahemi ◽  
Pratima Nangia-Makker ◽  
Vitaly Balan ◽  
Matteo Morelli ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Enzyme Design ◽  
Design Synthesis ◽  
Ubiquitin Conjugating Enzyme ◽  
Conjugating Enzyme

scholarly journals Design, Synthesis, Structural and Functional Characterization of Novel Melanocortin Agonists Based on the Cyclotide Kalata B1

2012 ◽  
Vol 287 (48) ◽  
pp. 40493-40501 ◽  
Author(s):  
Rasmus Eliasen ◽  
Norelle L. Daly ◽  
Birgitte S. Wulff ◽  
Thomas L. Andresen ◽  
Kilian W. Conde-Frieboes ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Design Synthesis ◽  
Kalata B1

Abstract 2270: Design, synthesis, and functional characterization of new cycle-peptides inhibitors for C-X-C chemokine receptor-4 (CXCR4)

2010 ◽  
Author(s):  
Stefania Scala ◽  
Luigi Portella ◽  
Maria Napolitano ◽  
Claudia Consales ◽  
Maria Neve Polimeno ◽  
...  
Keyword(s):  
Chemokine Receptor ◽  
Functional Characterization ◽  
Design Synthesis ◽  
Chemokine Receptor 4

Structural and Functional Characterization of DsbC, a Protein Involved in Disulfide Bond Formation in Escherichia coli

Biochemistry ◽  
1995 ◽  
Vol 34 (15) ◽  
pp. 5075-5089 ◽  
Author(s):  
Andre Zapun ◽  
Dominique Missiakas ◽  
Satish Raina ◽  
Thomas E. Creighton
Keyword(s):  
Escherichia Coli ◽  
Disulfide Bond ◽  
Bond Formation ◽  
Functional Characterization ◽  
Disulfide Bond Formation

A Novel Reconstitution Method for Inducing the Formation of Regular 2-Dimensional Arrays of Membrane Proteins and Lipids

1988 ◽  
Vol 46 ◽  
pp. 152-153 ◽  
Author(s):  
A. Engel ◽  
A. Holzenburg ◽  
K. Stauffer ◽  
J. Rosenbusch ◽  
U. Aebi
Keyword(s):  
Membrane Proteins ◽  
Flow Rate ◽  
Lipid Membranes ◽  
Functional Characterization ◽  
Dimensional Structure ◽  
Electron Crystallography ◽  
Peltier Element ◽  
Protein Ratio ◽  
Precise Control ◽  
3 Dimensional

Reconstitution of solubilized and purified membrane proteins in the presence of phospholipids into vesicles allows their functions to be studied by simple bulk measurements (e.g. diffusion of differently sized solutes) or by conductance measurements after transformation into planar membranes. On the other hand, reconstitution into regular protein-lipid arrays, usually forming at a specific lipid-to-protein ratio, provides the basis for determining the 3-dimensional structure of membrane proteins employing the tools of electron crystallography.To refine reconstitution conditions for reproducibly inducing formation of large and highly ordered protein-lipid membranes that are suitable for both electron crystallography and patch clamping experiments aimed at their functional characterization, we built a flow-dialysis device that allows precise control of temperature and flow-rate (Fig. 1). The flow rate is generated by a peristaltic pump and can be adjusted from 1 to 500 ml/h. The dialysis buffer is brought to a preselected temperature during its travel through a meandering path before it enters the dialysis reservoir. A Z-80 based computer controls a Peltier element allowing the temperature profile to be programmed as function of time.

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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