scholarly journals CRISPR-Cas-Mediated Tethering Recruits the Yeast HMR Mating-Type Locus to the Nuclear Periphery but Fails to Silence Gene Expression

2021 ◽  
Author(s):  
Emily R. Cliff ◽  
Robin L. Kirkpatrick ◽  
Daniel Cunningham-Bryant ◽  
Brianna Fernandez ◽  
Joseph L. Harman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Nuclear Periphery ◽  
Type Locus ◽  
Mating Type Locus ◽  
Silence Gene

scholarly journals Control of STA1 gene expression by the mating-type locus in yeasts.

1985 ◽  
Vol 164 (2) ◽  
pp. 769-773 ◽  
Author(s):  
I Yamashita ◽  
Y Takano ◽  
S Fukui
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus

scholarly journals CRISPR-Cas-mediated tethering recruits the yeast HMR mating-type locus to the nuclear periphery but fails to silence gene expression

2021 ◽  
Author(s):  
Emily R Cliff ◽  
Robin L Kirkpatrick ◽  
Daniel Cunningham-Bryant ◽  
Brianna Fernandez ◽  
Jesse G Zalatan
Keyword(s):  
Gene Expression ◽  
Genome Structure ◽  
Nuclear Periphery ◽  
Model Systems ◽  
Type Locus ◽  
Synthetic Genome ◽  
Structural Differences ◽  
And Function ◽  
The Relationship ◽  
Silence Gene

To investigate the relationship between genome structure and function, we have developed a programmable CRISPR-Cas system for nuclear peripheral recruitment in yeast. We benchmarked this system at the HMR and GAL2 loci, both well-characterized model systems for localization to the nuclear periphery. Using microscopy and gene silencing assays, we demonstrate that CRISPR-Cas-mediated tethering can recruit the HMR locus but does not silence reporter gene expression. A previously reported Gal4-mediated tethering system does silence gene expression, and we demonstrate that the silencing phenotype has an unexpected dependence on the structure of the protein tether. The CRISPR-Cas system was unable to recruit GAL2 to the nuclear periphery. Our results reveal potential challenges for synthetic genome structure perturbations and suggest that distinct functional effects can arise from subtle structural differences in how genes are recruited to the periphery.

Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML

1990 ◽  
Vol 10 (9) ◽  
pp. 4932-4934
Author(s):  
E C Park ◽  
J W Szostak
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Point Mutations ◽  
Active Role ◽  
Histone H4 ◽  
Type Locus ◽  
Mating Type Locus ◽  
N Terminus ◽  
Lysine Residues ◽  
Histone H4 Gene

The N-terminal serine and four conserved lysine residues near the N-terminus of yeast histone H4 are acetylated. We found that a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML, while mutations that altered the N-terminal serine or the first three lysines had only minor phenotypic effects. Our results support an active role for histone H4 in the silencing of gene expression at this locus.

scholarly journals Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.

1990 ◽  
Vol 10 (9) ◽  
pp. 4932-4934 ◽  
Author(s):  
E C Park ◽  
J W Szostak
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Point Mutations ◽  
Active Role ◽  
Histone H4 ◽  
Type Locus ◽  
Mating Type Locus ◽  
N Terminus ◽  
Lysine Residues ◽  
Histone H4 Gene

The N-terminal serine and four conserved lysine residues near the N-terminus of yeast histone H4 are acetylated. We found that a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML, while mutations that altered the N-terminal serine or the first three lysines had only minor phenotypic effects. Our results support an active role for histone H4 in the silencing of gene expression at this locus.

scholarly journals Coordination on timing of gene expression in Paramecium

1980 ◽  
Vol 36 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Koji Myohara
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Paramecium Caudatum ◽  
Wild Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Type Change ◽  
Early Maturing

SUMMARYIn Paramecium caudatum, syngen 3, cells become mature (i.e. capable of undergoing conjugation) by about 50 fissions after conjugation. Matingtype-instability, another mating characteristic, begins to occur between 80 and 120 fissions after conjugation in heterozygotes for the mating type locus (Myohara & Hiwatashi, 1975). Mating-type instability also occurs in dominant homozygotes, earlier than in heterozygotes. In two different early maturing mutants the homozygotes become mature 20 fissions earlier than in wild-type clones, and mating-type instabiliiy also begins earlier than in wild-type clones. The number of fissions from maturity to the occurrence of the mating type change does not differ.

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