Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML

1990 ◽  
Vol 10 (9) ◽  
pp. 4932-4934
Author(s):  
E C Park ◽  
J W Szostak
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Point Mutations ◽  
Active Role ◽  
Histone H4 ◽  
Type Locus ◽  
Mating Type Locus ◽  
N Terminus ◽  
Lysine Residues ◽  
Histone H4 Gene

The N-terminal serine and four conserved lysine residues near the N-terminus of yeast histone H4 are acetylated. We found that a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML, while mutations that altered the N-terminal serine or the first three lysines had only minor phenotypic effects. Our results support an active role for histone H4 in the silencing of gene expression at this locus.

scholarly journals Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.

1990 ◽  
Vol 10 (9) ◽  
pp. 4932-4934 ◽  
Author(s):  
E C Park ◽  
J W Szostak
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Point Mutations ◽  
Active Role ◽  
Histone H4 ◽  
Type Locus ◽  
Mating Type Locus ◽  
N Terminus ◽  
Lysine Residues ◽  
Histone H4 Gene

The N-terminal serine and four conserved lysine residues near the N-terminus of yeast histone H4 are acetylated. We found that a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML, while mutations that altered the N-terminal serine or the first three lysines had only minor phenotypic effects. Our results support an active role for histone H4 in the silencing of gene expression at this locus.

scholarly journals Control of STA1 gene expression by the mating-type locus in yeasts.

1985 ◽  
Vol 164 (2) ◽  
pp. 769-773 ◽  
Author(s):  
I Yamashita ◽  
Y Takano ◽  
S Fukui
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus

scholarly journals CRISPR-Cas-Mediated Tethering Recruits the Yeast HMR Mating-Type Locus to the Nuclear Periphery but Fails to Silence Gene Expression

2021 ◽  
Author(s):  
Emily R. Cliff ◽  
Robin L. Kirkpatrick ◽  
Daniel Cunningham-Bryant ◽  
Brianna Fernandez ◽  
Joseph L. Harman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Nuclear Periphery ◽  
Type Locus ◽  
Mating Type Locus ◽  
Silence Gene

scholarly journals SUM1-1: a suppressor of silencing defects in Saccharomyces cerevisiae.

Genetics ◽  
1991 ◽  
Vol 129 (3) ◽  
pp. 685-696 ◽  
Author(s):  
P Laurenson ◽  
J Rine
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Transcriptional Repression ◽  
Point Mutations ◽  
Histone H4 ◽  
Yeast Saccharomyces Cerevisiae ◽  
Type Locus ◽  
Sir Proteins ◽  
Silencer Elements ◽  
Was Gene

Abstract The repression of transcription of the silent mating-type locus HMRa in the yeast Saccharomyces cerevisiae requires the four SIR proteins, histone H4 and a flanking site designated HMR-E. The SUM1-1 mutation alleviated the need for many of these components in transcriptional repression. In the absence of each of the SIR proteins, SUM1-1 restored repression in MAT alpha strains; thus, SUM1-1 appeared to bypass the need for the SIR genes in repression of HMRa. Repression was not specific to the genes normally present at HMR, since the TRP1 gene placed at HMR was repressed by SUM1-1 in a sir3 strain. Therefore, like the mechanisms of silencing normally used at HMR, silencing by SUM1-1 was gene-nonspecific. SUM1-1 suppressed point mutations in histone H4, but failed to suppress strongly a deletion mutation in histone H4. Similarly, SUM1-1 suppressed mutations in the three known elements of HMR-E, but was unable to suppress a deletion of HMR-E. These epistasis analyses implied that the functions required for repression at HMR can be ordered, with the SIR genes and silencer elements acting upstream of SUM1-1. SUM1-1 itself may function at the level of chromatin in the assembly of inactive DNA at the silent mating-type loci.

scholarly journals Coordination on timing of gene expression in Paramecium

1980 ◽  
Vol 36 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Koji Myohara
Keyword(s):  
Gene Expression ◽  
Mating Type ◽  
Paramecium Caudatum ◽  
Wild Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Type Change ◽  
Early Maturing

SUMMARYIn Paramecium caudatum, syngen 3, cells become mature (i.e. capable of undergoing conjugation) by about 50 fissions after conjugation. Matingtype-instability, another mating characteristic, begins to occur between 80 and 120 fissions after conjugation in heterozygotes for the mating type locus (Myohara & Hiwatashi, 1975). Mating-type instability also occurs in dominant homozygotes, earlier than in heterozygotes. In two different early maturing mutants the homozygotes become mature 20 fissions earlier than in wild-type clones, and mating-type instabiliiy also begins earlier than in wild-type clones. The number of fissions from maturity to the occurrence of the mating type change does not differ.

Sign in / Sign up

Export Citation Format

Share Document