Preparation of Conjugates between Oligonucleotides andN-Vinylpyrrolidone/N-Acryloxysuccinimide Copolymers and Applications in Nucleic Acid Assays To Improve Sensitivity

1996 ◽  
Vol 7 (5) ◽  
pp. 568-575 ◽  
Author(s):  
Marie-Noëlle Erout ◽  
Alain Troesch ◽  
Christian Pichot ◽  
Philippe Cros
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Assays

Silver nanocluster-lightened hybridization chain reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57502-57506 ◽  
Author(s):  
Lin Liu ◽  
Qing Li ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Nucleic Acid ◽  
Silver Nanoclusters ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Silver Nanocluster ◽  
Turn On ◽  
Nucleic Acid Assays

A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.

Terminal Phosphate-Labeled Nucleotides with Improved Substrate Properties for Homogeneous Nucleic Acid Assays

2005 ◽  
Vol 127 (8) ◽  
pp. 2394-2395 ◽  
Author(s):  
Anup Sood ◽  
Shiv Kumar ◽  
Satyam Nampalli ◽  
John R. Nelson ◽  
John Macklin ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Substrate Properties ◽  
Nucleic Acid Assays

The hybridization chain reaction in the development of ultrasensitive nucleic acid assays

2015 ◽  
Vol 64 ◽  
pp. 86-99 ◽  
Author(s):  
Juleiha Ikbal ◽  
Geraldine S. Lim ◽  
Zhiqiang Gao
Keyword(s):  
Nucleic Acid ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Nucleic Acid Assays

Immobilized quantum dot bioprobes: Fiber optics and microfluidics for the development of nucleic acid assays

2011 ◽  
Author(s):  
A. J. Tavares ◽  
O. Noor ◽  
C. Vannoy ◽  
L. Chen ◽  
W. R. Algar ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantum Dot ◽  
Fiber Optics ◽  
Nucleic Acid Assays

Click chemistry as an immobilization method to improve oligonucleotide hybridization efficiency for nucleic acid assays

2016 ◽  
Vol 236 ◽  
pp. 286-293 ◽  
Author(s):  
Milena McKenna ◽  
Felipe Soberon ◽  
Antonio J. Ricco ◽  
Stephen Daniels ◽  
Susan M. Kelleher
Keyword(s):  
Nucleic Acid ◽  
Click Chemistry ◽  
Oligonucleotide Hybridization ◽  
Hybridization Efficiency ◽  
Nucleic Acid Assays ◽  
Immobilization Method

scholarly journals Efficient design of maximally active and specific nucleic acid diagnostics for thousands of viruses

2020 ◽  
Author(s):  
Hayden C. Metsky ◽  
Nicole L. Welch ◽  
Nicholas J. Haradhvala ◽  
Laurie Rumker ◽  
Yibin B. Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Genomic Diversity ◽  
Efficient Design ◽  
Full Spectrum ◽  
Diagnostic Assays ◽  
Genome Data ◽  
Species Specific ◽  
Nucleic Acid Assays ◽  
Lower Limits ◽  
Detection Technologies

AbstractHarnessing genomic data and predictive models will provide activity-informed diagnostic assays for thousands of viruses and offer rapid design for novel ones. Here we develop and extensively validate new algorithms that design nucleic acid assays having maximal predicted detection activity over a virus’s full genomic diversity with stringent specificity. Focusing on CRISPR-Cas13a detection, we test a library of ~ 19,000 guide-target pairs and construct a convolutional neural network that predicts Cas13a detection activity better than other techniques. We link our methods by building ADAPT, an end-to-end system that automatically leverages the latest viral genome data. We designed optimal species-specific assays for the 1,933 vertebrate-infecting viral species within 2 hours for most species and 24 hours for all but 3. ADAPT’s designs are sensitive and specific down to the lineage-level for the range of taxa we tested, including ones that pose challenges involving genomic diversity and specificity. They also exhibit significantly higher fluorescence and lower limits of detection, across a virus’s full spectrum of genomic diversity, than designs from standard techniques. ADAPT is available in an accessible software package and can be applied to other detection technologies to enhance critically-needed viral diagnostic and surveillance efforts.

scholarly journals The Promise of Aggregation-Induced Emission Luminogens for Detecting COVID-19

2021 ◽  
Vol 12 ◽  
Author(s):  
Zongwei Liu ◽  
Ting Meng ◽  
Xiaofang Tang ◽  
Ran Tian ◽  
Weijiang Guan
Keyword(s):  
Nucleic Acid ◽  
High Performance ◽  
Water Solubility ◽  
Detection Sensitivity ◽  
Quantitative Detection ◽  
Aggregation Induced Emission ◽  
Detection Of Biomolecules ◽  
Immunological Assays ◽  
Nucleic Acid Assays ◽  
Medical Therapeutics

The long-term pandemic of coronavirus disease 2019 (COVID-19) requires sensitive and accurate diagnostic assays to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and SARS-CoV-2 antibodies in infected individuals. Currently, RNA of SARS-CoV-2 virus is mainly detected by reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid assays, while SARS-CoV-2 antigen and antibody are identified by immunological assays. Both nucleic acid assays and immunological assays rely on the luminescence signals of specific luminescence probes for qualitative and quantitative detection. The exploration of novel luminescence probes will play a crucial role in improving the detection sensitivity of the assays. As innate probes, aggregation-induced emission (AIE) luminogens (AIEgens) exhibit negligible luminescence in the free state but enhanced luminescence in the aggregated or restricted states. Moreover, AIEgen-based nanoparticles (AIE dots) offer efficient luminescence, good biocompatibility and water solubility, and superior photostability. Both AIEgens and AIE dots have been widely used for high-performance detection of biomolecules and small molecules, chemical/biological imaging, and medical therapeutics. In this review, the availability of AIEgens and AIE dots in nucleic acid assays and immunological assays are enumerated and discussed. By building a bridge between AIE materials and COVID-19, we hope to inspire researchers to use AIE materials as a powerful weapon against COVID-19.

scholarly journals Scaling of nucleic acid assays on microelectrophoresis array devices: High-dynamic range multi-gene readout from less than ten transcripts

2009 ◽  
Vol 30 (12) ◽  
pp. 2090-2099
Author(s):  
Joern Ueberfeld ◽  
Daniel J. Ehrlich
Keyword(s):  
Nucleic Acid ◽  
Dynamic Range ◽  
High Dynamic Range ◽  
High Dynamic ◽  
Nucleic Acid Assays

Dna-Synthesis Studied in Later Growth Cycles and in Zoosporangium Development of Allomyces macrogynus

1988 ◽  
Vol 36 (3) ◽  
pp. 321 ◽  
Author(s):  
J Youatt
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Culture Medium ◽  
Morphological Differentiation ◽  
Growth Cycles ◽  
Apparent Increase ◽  
Partial Inhibition ◽  
Allomyces Macrogynus ◽  
Fourth Cycle ◽  
Nucleic Acid Assays

Synchrony of duplication cycles in Allomyces macrogynus was maintained through five cycles to the end of vegetative growth. In a culture in which hyphal emergence was inhibited the fourth cycle was abnormal. No DNA synthesis occurred before septation when zoosporangia developed in culture medium. When organisms were induced to make sporangia by transfer to water there was a small apparent increase in DNA prior to but not after septation. The unavoidable extraction of wall glycopeptide poses some problems in nucleic acid assays. Partial inhibition by 10�M benomyl allowed continued growth with large rhizoids at every apex. Partial inhibition by mitomycin allowed continued growth without any further morphological differentiation.

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