High-resolution structure of the temperature-sensitive mutant of phage lysozyme, Arg 96 .fwdarw. His

Biochemistry ◽  
1989 ◽  
Vol 28 (9) ◽  
pp. 3793-3797 ◽  
Author(s):  
L. H. Weaver ◽  
T. M. Gray ◽  
M. G. Gruetter ◽  
D. E. Anderson ◽  
J. A. Wozniak ◽  
...  
Keyword(s):  
High Resolution ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
High Resolution Structure ◽  
Resolution Structure

scholarly journals Dimeric and high-resolution structures of Chlamydomonas Photosystem I from a temperature-sensitive Photosystem II mutant

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Ido Caspy ◽  
Tom Schwartz ◽  
Vinzenz Bayro-Kaiser ◽  
Mariia Fadeeva ◽  
Amit Kessel ◽  
...  
Keyword(s):  
High Resolution ◽  
Photosystem Ii ◽  
Photosystem I ◽  
Water Molecules ◽  
Temperature Sensitive ◽  
Asymmetric Distribution ◽  
Light Harvesting Complexes ◽  
Transport Chain ◽  
High Resolution Structure ◽  
Resolution Structure

AbstractWater molecules play a pivotal functional role in photosynthesis, primarily as the substrate for Photosystem II (PSII). However, their importance and contribution to Photosystem I (PSI) activity remains obscure. Using a high-resolution cryogenic electron microscopy (cryo-EM) PSI structure from a Chlamydomonas reinhardtii temperature-sensitive photoautotrophic PSII mutant (TSP4), a conserved network of water molecules - dating back to cyanobacteria - was uncovered, mainly in the vicinity of the electron transport chain (ETC). The high-resolution structure illustrated that the water molecules served as a ligand in every chlorophyll that was missing a fifth magnesium coordination in the PSI core and in the light-harvesting complexes (LHC). The asymmetric distribution of the water molecules near the ETC branches modulated their electrostatic landscape, distinctly in the space between the quinones and FX. The data also disclosed the first observation of eukaryotic PSI oligomerisation through a low-resolution PSI dimer that was comprised of PSI-10LHC and PSI-8LHC.

Sign in / Sign up

Export Citation Format

Share Document