Receptor interconversion model of hormone action. 3. Estrogen receptor mediated repression of reporter gene activity in A431 cells

Biochemistry ◽  
1990 ◽  
Vol 29 (11) ◽  
pp. 2699-2702 ◽  
Author(s):  
Abhijit Nag ◽  
Insoo Park ◽  
Andree Krust ◽  
Roy G. Smith
Keyword(s):  
Estrogen Receptor ◽  
Reporter Gene ◽  
Gene Activity ◽  
Hormone Action ◽  
A431 Cells ◽  
Reporter Gene Activity

scholarly journals Response element sequence modulates estrogen receptor alpha and beta affinity and activity

2002 ◽  
Vol 29 (1) ◽  
pp. 137-152 ◽  
Author(s):  
PC Kulakosky ◽  
MA McCarty ◽  
SC Jernigan ◽  
KE Risinger ◽  
CM Klinge
Keyword(s):  
Estrogen Receptor ◽  
Reporter Gene ◽  
Estrogen Receptor Alpha ◽  
Gene Activity ◽  
Flanking Sequence ◽  
Response Element ◽  
Reporter Gene Activity ◽  
Estrogen Response ◽  
Transcriptional Induction

The relationship between estrogen receptor (ER)-estrogen response element (ERE) binding affinity and estradiol (E(2))-induced transcription has not been systematically or quantitatively tested. We examined the influence of ERE palindrome length and the 3' ERE flanking sequence on ERalpha and ERbeta affinity binding in vitro and on the induction of reporter gene activity in transfected cells. The addition of one nucleotide in each arm of the 13 bp ERE palindrome, forming a 15 bp ERE palindrome, increased ERalpha and ERbeta affinity and transcription. In contrast, the addition of an AT-rich flanking sequence from genes highly stimulated by E(2) had little effect on affinity or reporter gene activity. Notable differences between ERalpha and ERbeta include: both K(d) and transcriptional induction were generally higher for ERalpha than ERbeta, better correlation between ERE palindrome length and transcriptional induction for ERalpha than ERbeta, and a better correlation between (ER-ERE)K(d) and transcriptional induction for ERalpha than for ERbeta.

Isotopic Assays for Reporter Gene Activity

2001 ◽  
Vol 63 (1) ◽  
Author(s):  
Robert E. Kingston ◽  
Jen Sheen ◽  
David Moore
Keyword(s):  
Reporter Gene ◽  
Gene Activity ◽  
Reporter Gene Activity

scholarly journals Alternative promoters and repetitive DNA elements define the species-dependent tissue-specific expression of the FMO1 genes of human and mouse

2007 ◽  
Vol 406 (3) ◽  
pp. 491-499 ◽  
Author(s):  
Elizabeth A. Shephard ◽  
Pritpal Chandan ◽  
Milena Stevanovic-Walker ◽  
Mina Edwards ◽  
Ian R. Phillips
Keyword(s):  
Reporter Gene ◽  
Adult Mouse ◽  
Direct Repeat ◽  
Mouse Gene ◽  
Gene Activity ◽  
Upstream Sequence ◽  
Alternative Promoters ◽  
Specific Expression ◽  
Tissue Specific ◽  
Reporter Gene Activity

In humans, expression of the FMO1 (flavin-containing mono-oxygenase 1) gene is silenced postnatally in liver, but not kidney. In adult mouse, however, the gene is active in both tissues. We investigated the basis of this species-dependent tissue-specific transcription of FMO1. Our results indicate the use of three alternative promoters. Transcription of the gene in fetal human and adult mouse liver is exclusively from the P0 promoter, whereas in extra-hepatic tissues of both species, P1 and P2 are active. Reporter gene assays showed that the proximal P0 promoters of human (hFMO1) and mouse (mFmo1) genes are equally effective. However, sequences upstream (−2955 to −506) of the proximal P0 of mFmo1 increased reporter gene activity 3-fold, whereas hFMO1 upstream sequences (−3027 to −541) decreased reporter gene activity by 75%. Replacement of the upstream sequence of human P0 with the upstream sequence of mouse P0 increased activity of the human proximal P0 8-fold. Species-specific repetitive elements are present immediately upstream of the proximal P0 promoters. The human gene contains five LINE (long-interspersed nuclear element)-1-like elements, whereas the mouse gene contains a poly A region, an 80-bp direct repeat, an LTR (long terminal repeat), a SINE (short-interspersed nuclear element) and a poly T tract. The rat and rabbit FMO1 genes, which are expressed in adult liver, lack some (rat) or all (rabbit) of the elements upstream of mouse P0. Thus silencing of FMO1 in adult human liver is due apparently to the presence upstream of the proximal P0 of L1 (LINE-1) elements rather than the absence of retrotransposons similar to those found in the mouse gene.

scholarly journals Human Serum Induces Streptococcal C5a Peptidase Expression

2009 ◽  
Vol 77 (9) ◽  
pp. 3817-3825 ◽  
Author(s):  
Ute Gleich-Theurer ◽  
Simone Aymanns ◽  
Gregor Haas ◽  
Stefanie Mauerer ◽  
Julia Vogt ◽  
...  
Keyword(s):  
Human Serum ◽  
Reporter Gene ◽  
Bovine Serum ◽  
Calf Serum ◽  
Gene Activity ◽  
Pcr Analysis ◽  
Reporter Gene Activity ◽  
Reverse Transcription Pcr ◽  
Bovine Strain ◽  
Human Infections

ABSTRACTStreptococcus agalactiaeis a major pathogen in humans and animals. Virulence factors are often associated with mobile genetic elements, and their expression can be modulated by host factors.S. agalactiaeharbors the genes for C5a peptidase (scpB) and Lmb on a composite transposon structure which is absent in many bovine isolates. To investigate whether these genes participate in the adaptation to human hosts, we determined the influence of human and bovine serum on the promoter activity ofscpBandlmbby using fluorescence-activated cell sorter analysis. Culture in the presence of 1 to 50% human serum resulted in a dose-dependent induction of reporter gene activity forscpBbut notlmb. Reporter gene activity was, however, unchanged following growth in fetal calf serum. Interestingly, a bovine strain did not display any induction ofscpBby either bovine or human serum. Reverse transcription-PCR analysis was used to confirm differential induction ofscpBinS. agalactiaeand showed a similar induction of theStreptococcus pyogenesC5a peptidase genescpAby human but not bovine serum. The specific induction of the streptococcal C5a peptidase by human serum corresponds to the absence ofscpBin many bovineS. agalactiaeisolates and underlines the importance of this virulence factor for human infections.

scholarly journals A simple protocol for preparation of a liposomal vesicle with encapsulated plasmid DNA that mediate high accumulation and reporter gene activity in tumor tissue

2011 ◽  
Vol 1 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Torben Gjetting ◽  
Thomas Lars Andresen ◽  
Camilla Laulund Christensen ◽  
Frederik Cramer ◽  
Thomas Tuxen Poulsen ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Plasmid Dna ◽  
Tumor Tissue ◽  
Gene Activity ◽  
High Accumulation ◽  
Reporter Gene Activity ◽  
Simple Protocol

Cytochrome P4501A induction in avian hepatocyte cultures exposed to polychlorinated biphenyls: Comparisons with AHR1-mediated reporter gene activity and in ovo toxicity

2013 ◽  
Vol 266 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Gillian E. Manning ◽  
Lukas J. Mundy ◽  
Doug Crump ◽  
Stephanie P. Jones ◽  
Suzanne Chiu ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Reporter Gene ◽  
Gene Activity ◽  
In Ovo ◽  
Reporter Gene Activity ◽  
Hepatocyte Cultures ◽  
Cytochrome P4501a

A Method for Obtaining Reporter Gene Activity and Nuclear Extracts Simultaneously from Transiently Transfected Cells

1998 ◽  
Vol 265 (1) ◽  
pp. 185-187 ◽  
Author(s):  
Sanjaya Singh ◽  
Grady F. Saunders
Keyword(s):  
Reporter Gene ◽  
Gene Activity ◽  
Transfected Cells ◽  
Reporter Gene Activity ◽  
Nuclear Extracts
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