Thermal Induction of an Alternatively Folded State in Human IgG-Fc

Biochemistry ◽  
2011 ◽  
Vol 50 (6) ◽  
pp. 981-988 ◽  
Author(s):  
Daniel Kanmert ◽  
Ann-Christin Brorsson ◽  
Bengt-Harald Jonsson ◽  
Karin Enander
Keyword(s):  
Author(s):  
Yuru Shang ◽  
Xianbin Zhang ◽  
Lili Lu ◽  
Ke Jiang ◽  
Mathias Krohn ◽  
...  

Abstract Background Recent evidence proves that intravenous human immunoglobulin G (IgG) can impair cancer cell viability. However, no study evaluated whether IgG application benefits cancer patients receiving chemotherapeutics. Methods Influence of pharmaceutical-grade human IgG on the viability of a series of patient-derived colon cancer cell lines with and without chemotherapeutic intervention was determined. Cell death was analysed flow cytometrically. In addition, the influence of oxaliplatin and IgG on the ERK1/2-signalling pathway was evaluated by western blots. Results We evaluated the effects of pharmaceutical IgG, such as PRIVIGEN® IgG and Tonglu® IgG, in combination with chemotherapeutics. We did not observe any significant effects of IgG on tumour cell viability directly; however, human IgG significantly impaired the anti-tumoral effects of oxaliplatin. Primary cancer cell lines express IgG receptors and accumulate human IgG intracellularly. Moreover, while oxaliplatin induced the activation of ERK1/2, the pharmaceutical IgG inhibited ERK1/2 activity. Conclusions The present study demonstrates that pharmaceutical IgG, such as PRIVIGEN® IgG and Tonglu® IgG, can impair the anti-carcinoma activity of oxaliplatin. These data strongly suggest that therapeutic IgG as co-medication might have harmful side effects in cancer patients. The clinical significance of these preclinical observations absolutely advises further preclinical, as well as epidemiological and clinical research.


2003 ◽  
Vol 130 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Patricia Berasain ◽  
Carlos Carmona ◽  
Blas Frangione ◽  
Juan José Cazzulo ◽  
Fernando Goñi

1981 ◽  
Vol 57 (2) ◽  
pp. 377-388 ◽  
Author(s):  
Takatoshi Kuhara ◽  
Masamichi Hosono ◽  
Michio Fujiwara

1997 ◽  
Vol 16 (1-2) ◽  
pp. 29-55 ◽  
Author(s):  
Ingmar A. F. M. Heijnen ◽  
Jan G. J. Van de Winkel
Keyword(s):  

1986 ◽  
Vol 87 (1) ◽  
pp. 95-102 ◽  
Author(s):  
S.J. Challacombe ◽  
M. Biggerstaff ◽  
C. Greenall ◽  
D.M. Kemeny

2015 ◽  
Vol 7 (269) ◽  
pp. 269ra1-269ra1 ◽  
Author(s):  
C. Schneider ◽  
D. F. Smith ◽  
R. D. Cummings ◽  
K. F. Boligan ◽  
R. G. Hamilton ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document