Restriction Fragment Length Analysis of the Cytochrome b Gene and Muscle Fatty Acid Composition Differentiate the Cryptic Flatfish Species Solea solea and Solea aegyptiaca

ABSTRACT The levels of relatedness of strains of Xanthomonas fragariae collected over several years from locations in Canada and the United States were compared by determining fatty acid methyl ester profiles, restriction fragment length polymorphisms (RFLP) based on pulsed-field gel electrophoresis (PFGE) analysis, and DNA-DNA reassociation values. Based on qualitative and quantitative differences in fatty acid profiles, the strains were divided into nine groups and four groups by the MIDI “10% rule” and unweighted pair analysis, respectively. Restriction analysis of genomic DNA by PFGE with two endonucleases (XbaI and SpeI) revealed four distinct profiles. When a third endonuclease (VspI) was used, one group was divided into three subgroups. The profile of the American Type Culture Collection type strain differed from the profile of every other strain of X. fragariae. Considerable diversity was observed within X. fragariae, although the majority of the strains represented a clonal population. The four groups based on fatty acid profiles were similar to the four groups based on RFLP, but neither method related groups to the geographic origins of the strains. The DNA-DNA reassociation values were high for representative strains, providing evidence that all of the strains belong to the same species.

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Size class homogeneity of repeat lengths and evolutionary divergence of ribosomal RNA genes in fishes as studied by restriction fragment length analysis

Journal of Genetics ◽

10.1007/bf02927867 ◽

1991 ◽

Vol 70 (3) ◽

pp. 169-179 ◽

Cited By ~ 3

Author(s):

Bidyut Ghosh ◽

Utpal Datta ◽

Susanta Roy Choudhury ◽

Radha K. Mandal

Keyword(s):

Restriction Fragment ◽

Ribosomal Rna ◽

Fragment Length ◽

Size Class ◽

Ribosomal Rna Genes ◽

Evolutionary Divergence ◽

Length Analysis ◽

Rna Genes ◽

Restriction Fragment Length

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Identification of Mosquito Bloodmeal Source by Terminal Restriction Fragment Length Polymorphism Profile Analysis of the Cytochrome B Gene

Journal of Medical Entomology ◽

10.1093/jmedent/42.4.657 ◽

2005 ◽

Vol 42 (4) ◽

pp. 657-667 ◽

Cited By ~ 17

Author(s):

Jennifer K. Meece ◽

Cory E. Reynolds ◽

Patrick J. Stockwell ◽

Terra A. Jenson ◽

Jeffrey E. Christensen ◽

...

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Cytochrome B ◽

Restriction Fragment ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Profile Analysis ◽

Cytochrome B Gene ◽

Restriction Fragment Length

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Keragaman Gen Cytochrome B Pada Sidat (Anguila bicolor) Berdasarkan Restriction Fragment Length Polymorphism (RFLP)

Jurnal Biosains Pascasarjana ◽

10.20473/jbp.v20i3.2018.194-201 ◽

2018 ◽

Vol 20 (3) ◽

pp. 194

Author(s):

Lestari Wilujeng

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Cytochrome B ◽

Restriction Fragment ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Cyt B ◽

Program Software ◽

Restriction Fragment Length ◽

Pcr Primer

ABSTRAKPenelitian ini bertujuan untuk menganalisis karakter genetik Anguilla bicolor berdasarkan gen cytochrome b sebagai informasi dasar dalam studi filogeni dan rekayasa genetika. Penelitian telah dilaksanakan pada bulan Mei sampai September 2013 di Laboratorium Bioteknologi Fakultas MIPA Universitas Brawijaya. Penelitian ini menggunakan metode survei dengan analisis deskriptif kualitatif di laboratorium. Sampel diperoleh dari penangkapan langsung di Pantai Popo Tulungagung, Manado, Medan dan Ciamis. .Penelitian ini dimulai dengan isolasi DNA menggunakan metode CTAB dan dilanjutkan dengan PCR. Primer yang digunakan yaitu cytb-1(5’-TGCTAACGATGCCCTAGTGG-3’) dan cyt b-2 (5’-CTAGTCAACCTACT-AATGGG-3’). Hasil PCR dipotong dengan menggunakan enzyme retriksi Hha1 dan Msp1. Analisis data dilakukan dengan bantuan program Software NTSYS. Karakter genetik berupa urutan basa nukleotida penyusun DNA dari gen cytochrome b yang diperoleh pada masing-masing sampel memiliki tingkat kesamaan sekitar 32 – 100 %.

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