Release of Small Phenolic Compounds from Brewer’s Spent Grain and Its Lignin Fractions by Human Intestinal Microbiota in Vitro

2013 ◽  
Vol 61 (40) ◽  
pp. 9744-9753 ◽  
Author(s):  
Anna-Marja Aura ◽  
Piritta Niemi ◽  
Ismo Mattila ◽  
Klaus Niemelä ◽  
Annika Smeds ◽  
...  
2017 ◽  
Vol 39 (3) ◽  
pp. 269 ◽  
Author(s):  
Aline Da Rosa Almeida ◽  
Márcia Regina Ferreira Geraldo ◽  
Leomara Floriano Ribeiro ◽  
Marcos Vieira Silva ◽  
Matheus Vinicius De Oliveira Brisola Maciel ◽  
...  

 Brewer's spent grain (BSG) was characterized by physicochemical, total phenolic compound and flavonoids contents. Antioxidant activity was evaluated by four different assays. The chromatographic analyses were used to quantify the phenolic compounds and the fatty acids in BSG. Ethanolic extracts were tested to evaluate antibacterial activity. The higher concentration of total phenolic compounds for BSG was obtained in the extraction with ethanol 20%. BSG showed an antioxidant potential for all tests evaluated. In the case of chromatographic analysis, phenolic acids and flavonoids, such as syringic acid and catechin, respectively, were detected in high quantities. Regarding to the fatty acids profile, polyunsaturated fatty acids, such as linoleic and oleic acids, were found in significant amounts. No antibacterial activity was reported for bacterial cultures and concentrations tested. BSG may be considered a protein source, rich in fiber, polyunsaturated fatty acids and bioactive compounds with antioxidant potential. 


Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.


LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110136 ◽  
Author(s):  
Ingrid da Costa Maia ◽  
Carolina Thomaz dos Santos D'Almeida ◽  
Denise Maria Guimarães Freire ◽  
Elisa d'Avila Costa Cavalcanti ◽  
Luiz Claudio Cameron ◽  
...  

2013 ◽  
Vol 61 (27) ◽  
pp. 6754-6762 ◽  
Author(s):  
Piritta Niemi ◽  
Anna-Marja Aura ◽  
Johanna Maukonen ◽  
Annika I. Smeds ◽  
Ismo Mattila ◽  
...  

2013 ◽  
Vol 54 (2) ◽  
pp. 1589-1595 ◽  
Author(s):  
Xin Zhang ◽  
Xiuling Zhu ◽  
Yongkang Sun ◽  
Bing Hu ◽  
Yi Sun ◽  
...  

2016 ◽  
Vol 7 (4) ◽  
pp. 1959-1967 ◽  
Author(s):  
Li Zhou ◽  
Wei Wang ◽  
Jun Huang ◽  
Yu Ding ◽  
Zhouqiang Pan ◽  
...  

The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated.


2010 ◽  
Vol 58 (18) ◽  
pp. 10236-10246 ◽  
Author(s):  
Gabriele Gross ◽  
Doris M. Jacobs ◽  
Sonja Peters ◽  
Sam Possemiers ◽  
John van Duynhoven ◽  
...  

2020 ◽  
Author(s):  
Lei Liu ◽  
Hongmei Qi ◽  
Pengcheng Suo ◽  
Huai Lin ◽  
Siyi Wang ◽  
...  

Abstract Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.


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