Rapid Detection and Differentiation ofAlicyclobacillusSpecies in Fruit Juice Using Hydrophobic Grid Membranes and Attenuated Total Reflectance Infrared Microspectroscopy

2009 ◽  
Vol 57 (22) ◽  
pp. 10670-10674 ◽  
Author(s):  
Elizabeth M. Grasso ◽  
Ahmed E. Yousef ◽  
Silvia de Lamo Castellvi ◽  
Luis E. Rodriguez-Saona
Keyword(s):  
Rapid Detection ◽  
Fruit Juice ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Infrared Microspectroscopy

Rapid Differentiation of Bacillus Strains Using Hydrophobic Grid Membranes and Attenuated Total Reflectance Infrared Microspectroscopy

2009 ◽  
Vol 72 (9) ◽  
pp. 1909-1915 ◽  
Author(s):  
ELIZABETH M. GRASSO ◽  
AHMED E. YOUSEF ◽  
LUIS A. RODRIGUEZ-ROMO ◽  
LUIS E. RODRIGUEZ-SAONA
Keyword(s):  
Food Industry ◽  
Strain Level ◽  
Attenuated Total Reflectance ◽  
Bacillus Species ◽  
Total Reflectance ◽  
Infrared Microspectroscopy ◽  
Molecular Identification Methods ◽  
Ir Microspectroscopy ◽  
Multivariate Pattern

Bacillus species may be resistant to processing and sanitation procedures, making their control an important issue in the food industry. The objective of this study was to develop a rapid method for the differentiation of Bacillus cells at the strain level using infrared microspectroscopy and multivariate pattern recognition techniques. Aliquots (10 ml) of vegetative cells (~103 CFU/ml) from four strains of each of three Bacillus species (B. cereus, B. mycoides, and B. thuringiensis) were filtered onto hydrophobic grid membranes. The membranes were placed on tryptic soy agar and incubatedat 42°C for 24 h and then removed from the agar and dried, and the biomass of individual vegetative colonies was directly measured by attenuated total reflectance infrared (ATRIR) microspectroscopy. Soft independent modeling of class analogy models generated from second derivative transformed spectra in the 1,300 to 900 cm−1 region exhibited clusters that permitted accurate strain-level classification of all isolates. Major discrimination was related to the signal from phosphate-containing compounds, likely phospholipids. Results indicate that a simple ATR-IR microspectroscopy technique combined with multivariate analysis could provide the food industry with a rapid and reagent-free screening procedure to complement more elaborate molecular identification methods.

Attenuated total reflectance infrared microspectroscopy of coal

Fuel ◽  
2000 ◽  
Vol 79 (6) ◽  
pp. 685-691 ◽  
Author(s):  
J. Thomasson ◽  
C. Coin ◽  
H. Kahraman ◽  
P.M. Fredericks
Keyword(s):  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Infrared Microspectroscopy
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