Synthesis, In Vitro Receptor Binding, and In Vivo Evaluation of Fluorescein and Carbocyanine Peptide-Based Optical Contrast Agents

2002 ◽  
Vol 45 (10) ◽  
pp. 2003-2015 ◽  
Author(s):  
Samuel Achilefu ◽  
Hermo N. Jimenez ◽  
Richard B. Dorshow ◽  
Joseph E. Bugaj ◽  
Elizabeth G. Webb ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Receptor Binding ◽  
Optical Contrast ◽  
In Vivo Evaluation ◽  
Optical Contrast Agents

scholarly journals In vivo multimodal magnetic particle imaging (MPI) with tailored magneto/optical contrast agents

Biomaterials ◽  
2015 ◽  
Vol 52 ◽  
pp. 251-261 ◽  
Author(s):  
Hamed Arami ◽  
Amit P. Khandhar ◽  
Asahi Tomitaka ◽  
Elaine Yu ◽  
Patrick W. Goodwill ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Magnetic Particle ◽  
Optical Contrast ◽  
Optical Contrast Agents ◽  
Magnetic Particle Imaging ◽  
Particle Imaging

scholarly journals Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours

2020 ◽  
Vol 2 (4) ◽  
pp. 1590-1602
Author(s):  
Geoffrey Prévot ◽  
Talia Bsaibess ◽  
Jonathan Daniel ◽  
Coralie Genevois ◽  
Guillaume Clermont ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Cancer Cells ◽  
In Vivo Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Optical Contrast ◽  
Optical Probes ◽  
Optical Contrast Agents

Tailor-made NIR emitting dyes were designed as multimodal optical probes.

In vivo tumor characterization using both MR and optical contrast agents with a hybrid MRI-DOT system

2011 ◽  
Author(s):  
Yuting Lin ◽  
Michael Ghijsen ◽  
David Thayer ◽  
Orhan Nalcioglu ◽  
Gultekin Gulsen
Keyword(s):  
Contrast Agents ◽  
Optical Contrast ◽  
Tumor Characterization ◽  
Optical Contrast Agents

scholarly journals Comparison of Folate Receptor Targeted Optical Contrast Agents for Intraoperative Molecular Imaging

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Elizabeth De Jesus ◽  
Jane J. Keating ◽  
Sumith A. Kularatne ◽  
Jack Jiang ◽  
Ryan Judy ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Animal Studies ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Folate Receptor ◽  
Intraoperative Imaging ◽  
Nir Dyes ◽  
Optical Contrast Agents

Background. Intraoperative imaging can identify cancer cells in order to improve resection; thus fluorescent contrast agents have emerged. Our objective was to do a preclinical comparison of two fluorescent dyes, EC17 and OTL38, which both target folate receptor but have different fluorochromes. Materials. HeLa and KB cells lines were used for in vitro and in vivo comparisons of EC17 and OTL38 brightness, sensitivity, pharmacokinetics, and biodistribution. In vivo experiments were then performed in mice. Results. The peak excitation and emission wavelengths of EC17 and OTL38 were 470/520 nm and 774/794 nm, respectively. In vitro, OTL38 required increased incubation time compared to EC17 for maximum fluorescence; however, peak signal-to-background ratio (SBR) was 1.4-fold higher compared to EC17 within 60 minutes (p<0.001). Additionally, the SBR for detecting smaller quantity of cells was improved with OTL38. In vivo, the mean improvement in SBR of tumors visualized using OTL38 compared to EC17 was 3.3 fold (range 1.48–5.43). Neither dye caused noticeable toxicity in animal studies. Conclusions. In preclinical testing, OTL38 appears to have superior sensitivity and brightness compared to EC17. This coincides with the accepted belief that near infrared (NIR) dyes tend to have less autofluorescence and scattering issues than visible wavelength fluorochromes.

In-vivo quantification of optical contrast agents using a combined frequency-domain and steady state technique

2002 ◽  
Author(s):  
David J. Cuccia ◽  
Frederic Bevilacqua ◽  
Anthony J. Durkin ◽  
Sean Merritt ◽  
Bruce J. Tromberg ◽  
...  
Keyword(s):  
Steady State ◽  
Contrast Agents ◽  
Frequency Domain ◽  
Optical Contrast ◽  
Optical Contrast Agents ◽  
State Technique

Confocal Microscopy and Optical Contrast Agents for In Vivo Detection Of Cancer

2008 ◽  
Vol 14 (S2) ◽  
pp. 728-729
Author(s):  
MC Pierce ◽  
DJ Javier ◽  
N Nitin ◽  
RT Kester ◽  
T Tkaczyk ◽  
...  
Keyword(s):  
New Mexico ◽  
Confocal Microscopy ◽  
Contrast Agents ◽  
Optical Contrast ◽  
Optical Contrast Agents ◽  
In Vivo Detection

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

Tantalum Oxide Nanoparticles as Versatile Contrast Agents for X-ray Computed Tomography

2020 ◽  
Author(s):  
Shatadru Chakravarty ◽  
Jeremy Hix ◽  
Kaitlyn Wieweora ◽  
Maximilian Volk ◽  
Elizabeth Kenyon ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Tantalum Oxide ◽  
High Signal ◽  
Drug Delivery Vehicles ◽  
X Ray Computed

Here we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaOx NCs demonstrate high CT contrast, circulation in blood for ~ 3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaOx@MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaOx@PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaOx@PLGA NPs and TaOx@MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formuated NPs demonstrate high cytocompatibility and low dissolution of TaOx. This work solidifies that TaOx-based NPs are versatile contrast agents for CT.

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