Covalent Inhibitors of Interleukin-2 Inducible T Cell Kinase (Itk) with Nanomolar Potency in a Whole-Blood Assay

2012 ◽  
Vol 55 (22) ◽  
pp. 10047-10063 ◽  
Author(s):  
Christoph W. Zapf ◽  
Brian S. Gerstenberger ◽  
Li Xing ◽  
David C. Limburg ◽  
David R. Anderson ◽  
...  
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Interleukin 2 ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Covalent Inhibitors ◽  
Inducible T Cell Kinase

scholarly journals Vδ2+γδ T Cell Function inMycobacterium tuberculosis–and HIV‐1–Positive Patients in the United States and Uganda: Application of a Whole‐Blood Assay

2005 ◽  
Vol 192 (10) ◽  
pp. 1806-1814 ◽  
Author(s):  
Roxana E. Rojas ◽  
Keith A. Chervenak ◽  
Jeremy Thomas ◽  
Jamila Morrow ◽  
Lorna Nshuti ◽  
...  
Keyword(s):  
United States ◽  
T Cell ◽  
Whole Blood ◽  
Cell Function ◽  
The United States ◽  
Γδ T Cell ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
T Cell Function ◽  
Hiv 1

Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity

1994 ◽  
Vol 176 (1) ◽  
pp. 93-101 ◽  
Author(s):  
R.E. Weir ◽  
A.R. Morgan ◽  
W.J. Britton ◽  
C.R. Butlin ◽  
H.M. Dockrell
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
T Cell Responses ◽  
Field Studies ◽  
Whole Blood Assay ◽  
Cell Responses ◽  
Blood Assay

scholarly journals T-cell biomarkers for diagnosis of tuberculosis: candidate evaluation by a simple whole blood assay for clinical translation

2018 ◽  
Vol 51 (3) ◽  
pp. 1800153 ◽  
Author(s):  
Munyaradzi Musvosvi ◽  
Darragh Duffy ◽  
Elizabeth Filander ◽  
Hadn Africa ◽  
Simbarashe Mabwe ◽  
...  
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Clinical Translation ◽  
Candidate Evaluation ◽  
Whole Blood Assay ◽  
Blood Assay

Gingerol derivatives modulate T-cell receptor mediated effects in a whole blood assay and in T-cells via 5-HT1A receptor-dependent mechanisms

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
A Nievergelt ◽  
R Schoop ◽  
KH Altmann ◽  
J Gertsch
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Whole Blood ◽  
Cell Receptor ◽  
Whole Blood Assay ◽  
Mediated Effects ◽  
Blood Assay

scholarly journals Uncovering Distinct Primary Vaccination-Dependent Profiles in Human Bordetella pertussis Specific CD4+ T-Cell Responses Using a Novel Whole Blood Assay

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 225
Author(s):  
Eleonora E. Lambert ◽  
Véronique Corbière ◽  
Jacqueline A. M. van Gaans-van den Brink ◽  
Maxime Duijst ◽  
Prashanna Balaji Venkatasubramanian ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Bordetella Pertussis ◽  
Whole Blood ◽  
T Cell Responses ◽  
Primary Vaccination ◽  
Cd4 T Cell ◽  
Whole Blood Assay ◽  
Cell Responses ◽  
Blood Assay

To advance research and development of improved pertussis vaccines, new immunoassays are needed to qualify the outcome of Bordetella pertussis (Bp) specific CD4+ T-cell differentiation. Here, we applied a recently developed whole blood assay to evaluate Bp specific CD4+ T-cell responses. The assay is based on intracellular cytokine detection after overnight in vitro Bp antigen stimulation of diluted whole blood. We show for the first time that CD4+ T-cell memory of Th1, Th2, and Th17 lineages can be identified simultaneously in whole blood. Participants ranging from 7 to 70 years of age with different priming backgrounds of whole-cell pertussis (wP) and acellular pertussis (aP) vaccination were analyzed around an acellular booster vaccination. The assay allowed detection of low frequent antigen-specific CD4+ T-cells and revealed significantly elevated numbers of activated and cytokine-producing CD4+ T-cells, with a significant tendency to segregate recall responses based on primary vaccination background. A stronger Th2 response hallmarked an aP primed cohort compared to a wP primed cohort. In conclusion, analysis of Bp specific CD4+ T-cell responses in whole blood showed separation based on vaccination background and provides a promising tool to assess the quantity and quality of CD4+ T-cell responses induced by vaccine candidates.

scholarly journals Rapid, simplified whole blood-based multiparameter assay to quantify and phenotype SARS-CoV-2 specific T cells

2020 ◽  
Author(s):  
Catherine Riou ◽  
Georgia Schäfer ◽  
Elsa du Bruyn ◽  
Rene T. Goliath ◽  
Cari Stek ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Whole Blood ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Proof Of Concept ◽  
Whole Blood Assay ◽  
Cell Responses ◽  
Vaccine Trials ◽  
Blood Assay

ABSTRACTRapid tests to evaluate SARS-CoV-2-specific T cell responses are urgently needed to decipher protective immunity and aid monitoring vaccine-induced immunity. Using a rapid whole blood assay requiring minimal amount of blood, we measured qualitatively and quantitatively SARS-CoV-2-specific CD4 T cell responses in 31 healthcare workers, using flow cytometry. 100% of COVID-19 convalescent participants displayed a detectable SARS-CoV-2-specific CD4 T cell response. SARS-CoV-2-responding cells were also detected in 40.9% of participants with no COVID-19-associated symptoms or who tested PCR negative. Phenotypic assessment indicated that, in COVID-19 convalescent participants, SARS-CoV-2 CD4 responses displayed an early differentiated memory phenotype with limited capacity to produce IFNγ. Conversely, in participants with no reported symptoms, SARS-CoV-2 CD4 responses were enriched in late differentiated cells, co-expressing IFNγ and TNFα and also Granzyme B. This proof of concept study presents a scalable alternative to PBMC-based assays to enumerate and phenotype SARS-CoV-2-responding T cells, thus representing a practical tool to monitor adaptive immunity in vaccine trials.SummaryIn this proof of concept study, we show that SARS-CoV-2 T cell responses are easily detectable using a rapid whole blood assay requiring minimal blood volume. Such assay could represent a suitable tool to monitor adaptive immunity in vaccine trials.

scholarly journals Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals

2004 ◽  
Vol 99 (1) ◽  
pp. 53-55 ◽  
Author(s):  
Paulo RZ Antas ◽  
Fernando LL Cardoso ◽  
Eliane B Oliveira ◽  
Patrícia KC Gomes ◽  
Kátia S Cunha ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Immune Responses ◽  
Whole Blood ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
T Cell Immune Responses
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