Study of the Morphological and Adhesion Properties of Collagen Fibers in the Bruch's Membrane

2005 ◽  
Vol 109 (41) ◽  
pp. 19052-19055 ◽  
Author(s):  
Shrestha Basu Mallick ◽  
Albena Ivanisevic
Keyword(s):  
Collagen Fibers ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Adhesion Properties

scholarly journals Binding of Fab-horseradish peroxidase conjugates by charge and not by immunospecificity.

1985 ◽  
Vol 33 (1) ◽  
pp. 55-58 ◽  
Author(s):  
R M Pino
Keyword(s):  
Horseradish Peroxidase ◽  
Isoelectric Focusing ◽  
Collagen Fibers ◽  
Enzyme Digestion ◽  
Nonspecific Binding ◽  
Anionic Sites ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Type Viii ◽  
Positively Charged

The influence of horseradish peroxidase (HRP) charge on Fab-HRP conjugates was investigated. Rabbit nonimmune Fab coupled via periodate or glutaraldehyde to Sigma HRP type VI (pI greater than 10) were cationic (positively charged) as determined by analytical isoelectric focusing. These conjugates and HRP type VI alone stippled the basal laminae and collagen fibers in Bruch's membrane of the rat eye in a pattern identical to anionic (negative) sites. Binding was not present after the anionic sites were removed by enzyme digestion prior to immunolabeling or when HRP type VIII (anionic with pI 3.6) was used in an Fab-HRP conjugate or in an unbound form. These results indicate that anionic HRPs should be used in Fab-HRP preparations if a nonspecific binding to anionic sites is possible.

Abcc6 deficiency in the mouse leads to calcification of collagen fibers in Bruch's membrane

2012 ◽  
Vol 104 ◽  
pp. 59-64 ◽  
Author(s):  
Theo G.M.F. Gorgels ◽  
Peter Teeling ◽  
Johannes D. Meeldijk ◽  
Suzan T.M. Nillesen ◽  
Allard C. van der Wal ◽  
...  
Keyword(s):  
Collagen Fibers ◽  
Bruch's Membrane ◽  
Bruch’S Membrane

Characterization of collagen fibers in Bruch’s membrane using chemical force microscopy

2006 ◽  
Vol 386 (3) ◽  
pp. 652-657 ◽  
Author(s):  
Shrestha Basu Mallick ◽  
Sandhya Bhagwandin ◽  
Albena Ivanisevic
Keyword(s):  
Collagen Fibers ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Force Microscopy ◽  
Chemical Force Microscopy ◽  
Chemical Force

scholarly journals FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rawshan Choudhury ◽  
Nadhim Bayatti ◽  
Richard Scharff ◽  
Ewa Szula ◽  
Viranga Tilakaratna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Fate ◽  
Retinal Pigment ◽  
Factor H ◽  
Age Related Macular Degeneration ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Rpe Cells ◽  
Age Related

AbstractRetinal pigment epithelial (RPE) cells that underlie the neurosensory retina are essential for the maintenance of photoreceptor cells and hence vision. Interactions between the RPE and their basement membrane, i.e. the inner layer of Bruch’s membrane, are essential for RPE cell health and function, but the signals induced by Bruch’s membrane engagement, and their contributions to RPE cell fate determination remain poorly defined. Here, we studied the functional role of the soluble complement regulator and component of Bruch’s membrane, Factor H-like protein 1 (FHL-1). Human primary RPE cells adhered to FHL-1 in a manner that was eliminated by either mutagenesis of the integrin-binding RGD motif in FHL-1 or by using competing antibodies directed against the α5 and β1 integrin subunits. These short-term experiments reveal an immediate protein-integrin interaction that were obtained from primary RPE cells and replicated using the hTERT-RPE1 cell line. Separate, longer term experiments utilising RNAseq analysis of hTERT-RPE1 cells bound to FHL-1, showed an increased expression of the heat-shock protein genes HSPA6, CRYAB, HSPA1A and HSPA1B when compared to cells bound to fibronectin (FN) or laminin (LA). Pathway analysis implicated changes in EIF2 signalling, the unfolded protein response, and mineralocorticoid receptor signalling as putative pathways. Subsequent cell survival assays using H2O2 to induce oxidative stress-induced cell death suggest hTERT-RPE1 cells had significantly greater protection when bound to FHL-1 or LA compared to plastic or FN. These data show a non-canonical role of FHL-1 in protecting RPE cells against oxidative stress and identifies a novel interaction that has implications for ocular diseases such as age-related macular degeneration.

Bruch's membrane opening minimum rim width and retinal nerve fiber layer helps differentiate compressive optic neuropathy from glaucoma

2021 ◽  
Author(s):  
Thais de Souza Andrade ◽  
Rafael Barbosa de Araújo ◽  
Arthur Andrade do Nascimento Rocha ◽  
Luiz Guilherme Marchesi Mello ◽  
Leonardo P. Cunha ◽  
...  
Keyword(s):  
Nerve Fiber ◽  
Optic Neuropathy ◽  
Retinal Nerve Fiber Layer ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Fiber Layer ◽  
Compressive Optic Neuropathy ◽  
Nerve Fiber Layer ◽  
Bruch’S Membrane Opening ◽  
Retinal Nerve Fiber

Cellular Processes Causing Defects in Bruch's Membrane Following Krypton Laser Photocoagulation

Ophthalmology ◽  
1986 ◽  
Vol 93 (8) ◽  
pp. 1113-1119 ◽  
Author(s):  
A. Pollack ◽  
W.J. Heriot ◽  
P. Henkind
Keyword(s):  
Laser Photocoagulation ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Cellular Processes

Comparative histogenesis of Bruch's membrane (complexus basalis)

1991 ◽  
Vol 53 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Jack V. Greiner ◽  
Thomas A. Weidman
Keyword(s):  
Bruch's Membrane ◽  
Bruch’S Membrane

Bruch’s membrane opening-minimum rim width: An alternative OCT biomarker study for multiple sclerosis

2021 ◽  
pp. 112067212199663
Author(s):  
Kemal Turgay Özbilen ◽  
Tuncay Gündüz ◽  
Selva Nur Çukurova Kartal ◽  
Ali Ceyhun Gedik ◽  
Mefküre Eraksoy ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
High Sensitivity ◽  
The Novel ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Spectral Domain Oct ◽  
Neuroretinal Rim ◽  
Bruch’S Membrane Opening ◽  
The Difference ◽  
Therapeutic Decision Making

Purpose: Bruch’s membrane opening-minimum rim width (BMO-MRW) and RNFL measured using anatomic positioning system (APS-RNFL) are novel OCT methods and remained unexplored in MS patients. To investigate the novel parameters of spectral-domain OCT as an alternative biomarker in patients with multiple sclerosis (MS). Methods: Retrospective cohort study; participants consisted of relapsing-remitting MS (RRMS) patients and healthy controls (HC). Eyes were classified according to the presence of MS and previous optic neuritis (ON). Measurements of standard peripapillary RNFL (S-RNFL), BMO-MRW, and APS-RNFL were performed. Result: A total of 244 eyes of 122 participants (MS-patients: 63, HC: 59) were included in the study. Fifty-one eyes had a history of previous ON. In almost all measured parameters, neuroretinal rim thicknesses were observed the thinnest in eyes with ON history between all subgroups. S-RNFL and APS-RNFL techniques showed the difference in neuroretinal rim thickness in all three subjects (ON+, ON−, and HC). However, BMO-MRW, on the other hand, could not distinguish between ON(−) patients and HC. The relationship between OCT parameters and EDSS were observed only in eyes with an ON history in all three techniques. A meaningful model with 78% accuracy was obtained by using only the OCT parameters as risk factors. In the ROC analysis, no parameters were found to have acceptable high sensitivity and specificity. BMO-MRW was statistically weaker in every aspect than other RNFL techniques. Conclusion: The novel APS-RNFL technique appears to be a bit more reliable alternative to S-RNFL technique to support therapeutic decision-making in MS. BMO-MRW has not been found as a successful alternative to S-RNFL.

scholarly journals Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice

2018 ◽  
Vol 257 (2) ◽  
pp. 289-301 ◽  
Author(s):  
Hidetsugu Mori ◽  
Haruhiko Yamada ◽  
Keiko Toyama ◽  
Kanji Takahashi ◽  
Tomoya Akama ◽  
...  
Keyword(s):  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Age Related ◽  
Elastic Lamina ◽  
Age Related Changes
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