scholarly journals Translational Diffusion Measurements by Microcoil NMR in Aqueous Solutions of the Fos-10 Detergent-Solubilized Membrane Protein OmpX

2012 ◽  
Vol 116 (23) ◽  
pp. 6775-6780 ◽  
Author(s):  
Reto Horst ◽  
Pawel Stanczak ◽  
Pedro Serrano ◽  
Kurt Wüthrich
Keyword(s):  
Membrane Protein ◽  
Aqueous Solutions ◽  
Translational Diffusion ◽  
Microcoil Nmr

Translational Diffusion of Paramagnetic Tracers in Poly(1-vinylpyrrolidone) Hydrogel and Concentrated Aqueous Solutions by 1D Electron Spin Resonance Imaging

1999 ◽  
Vol 32 (24) ◽  
pp. 8230-8233 ◽  
Author(s):  
Jan Pilař ◽  
Jiří Labský ◽  
Antonín Marek ◽  
Čestmír Koňák ◽  
Shulamith Schlick
Keyword(s):  
Electron Spin Resonance ◽  
Aqueous Solutions ◽  
Electron Spin ◽  
Translational Diffusion ◽  
Resonance Imaging ◽  
Concentrated Aqueous Solutions ◽  
Spin Resonance

Translational Diffusion Coefficients of Volatile Compounds in Various Aqueous Solutions at Low and Subzero Temperatures

2005 ◽  
Vol 53 (17) ◽  
pp. 6771-6776 ◽  
Author(s):  
Marco Covarrubias-Cervantes ◽  
Dominique Champion ◽  
Frédéric Debeaufort ◽  
Andrée Voilley
Keyword(s):  
Aqueous Solutions ◽  
Volatile Compounds ◽  
Diffusion Coefficients ◽  
Translational Diffusion ◽  
Subzero Temperatures

scholarly journals Effect of Reducing Agent TCEP on Translational Diffusion and Supramolecular Assembly in Aqueous Solutions of α-Casein

2019 ◽  
Vol 123 (10) ◽  
pp. 2305-2315 ◽  
Author(s):  
Daria L. Melnikova ◽  
Vladimir D. Skirda ◽  
Irina V. Nesmelova
Keyword(s):  
Aqueous Solutions ◽  
Supramolecular Assembly ◽  
Translational Diffusion ◽  
Reducing Agent

Translational Diffusion of Dilute Aqueous Solutions of Sugars as Probed by NMR and Hydrodynamic Theory

2001 ◽  
Vol 105 (43) ◽  
pp. 9827-9833 ◽  
Author(s):  
Céline Monteiro ◽  
Catherine Hervé du Penhoat
Keyword(s):  
Aqueous Solutions ◽  
Translational Diffusion ◽  
Hydrodynamic Theory ◽  
Dilute Aqueous Solutions

Dynamic Light Scattering Investigation in Aqueous Solutions of bc1-Complex Membrane Protein

2002 ◽  
Vol 106 (16) ◽  
pp. 4318-4324 ◽  
Author(s):  
Kazuo Onuma ◽  
Tomomi Kubota ◽  
Shinpei Tanaka ◽  
Noriko Kanzaki ◽  
Atsuo Ito ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Membrane Protein ◽  
Aqueous Solutions ◽  
Bc1 Complex ◽  
Complex Membrane

scholarly journals Electrostatic long-range interactions in macromolecules of flexible-chain linear polyelectrolytes with low charge density in aqueous solutions of different ionic strength

2019 ◽  
Vol 489 (3) ◽  
pp. 262-266
Author(s):  
G. M. Pavlov ◽  
O. A. Dommes ◽  
O. V. Okatova ◽  
I. I. Gavrilova ◽  
E. F. Panarin
Keyword(s):  
Ionic Strength ◽  
Aqueous Solutions ◽  
Long Range ◽  
Translational Diffusion ◽  
Velocity Sedimentation ◽  
Scaling Relations ◽  
Nacl Solution ◽  
Flexible Chain ◽  
Long Range Interactions ◽  
Low Charge

The methods of molecular hydrodynamics (translational diffusion, velocity sedimentation, viscometry) have been used to study copolymers of N‑methyl-N‑vinylacetamide and N‑methyl-N‑vinylamine hydrochloride with an average content of charged groups (4,4 0,2) mol.% in aqueous 0,2 M NaCl solution. Kuhn-Mark-Houwink-Sakurada scaling relations were obtained. Viscous flow was studied in the widest possible range of ionic strengths of aqueous solutions, from salt-free to 6 M NaCl. The data got were compared with those previously obtained for neutral poly-N‑methyl-N‑vinylacetamide. It was firstly shown experimentally that the character of the dependence of the intrinsic viscosity on the molecular weight of a copolymer of such composition in solutions of minimal ionic strength is typical for the chains exhibiting intrachain volume effects, i.e. electrostatic long-range interactions.

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

Ultrathin frozen sections of yeast without aldehyde prefixation

1978 ◽  
Vol 36 (2) ◽  
pp. 58-59
Author(s):  
K. J. Böhm ◽  
a. E. Unger
Keyword(s):  
Aqueous Solutions ◽  
Biological Material ◽  
Yeast Cells ◽  
Frozen Sections ◽  
Good Preservation ◽  
Ultrathin Frozen Sections

During the last years it was shown that also by means of cryo-ultra-microtomy a good preservation of substructural details of biological material was possible. However the specimen generally was prefixed in these cases with aldehydes.Preparing ultrathin frozen sections of chemically non-prefixed material commonly was linked up to considerable technical and manual expense and the results were not always satisfying. Furthermore, it seems to be impossible to carry out cytochemical investigations by means of treating sections of unfixed biological material with aqueous solutions.We therefore tried to overcome these difficulties by preparing yeast cells (S. cerevisiae) in the following manner:

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

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