scholarly journals Proton Transport in a Membrane Protein Channel: Two-Dimensional Infrared Spectrum Modeling

2012 ◽  
Vol 116 (22) ◽  
pp. 6336-6345 ◽  
Author(s):  
Chungwen Liang ◽  
Jasper Knoester ◽  
Thomas L. C. Jansen
Keyword(s):  
Membrane Protein ◽  
Infrared Spectrum ◽  
Proton Transport ◽  
Two Dimensional ◽  
Protein Channel ◽  
Spectrum Modeling

Parameters for the Two-Dimensional Crystallization of the Membrane Protein Microsomal Glutathione Transferase

1998 ◽  
Vol 123 (2) ◽  
pp. 87-96 ◽  
Author(s):  
Ingeborg Schmidt-Krey ◽  
Gerd Lundqvist ◽  
Ralf Morgenstern ◽  
Hans Hebert
Keyword(s):  
Membrane Protein ◽  
Glutathione Transferase ◽  
Two Dimensional ◽  
Microsomal Glutathione

scholarly journals Proteomic analysis of the pulvinus, a heliotropic tissue, in Glycine max

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Hakme Lee ◽  
Wesley M. Garrett ◽  
Joseph Sullivan ◽  
Irwin Forseth ◽  
Savithiry S. Natarajan
Keyword(s):  
Mass Spectrometry ◽  
Gene Ontology ◽  
Gel Electrophoresis ◽  
Proton Transport ◽  
Enrichment Analysis ◽  
The Sun ◽  
Leaf Movement ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Leguminous Plants

Certain plant species respond to light, dark, and other environmental factors by leaf movement. Leguminous plants both track and avoid the sun through turgor changes of the pulvinus tissue at the base of leaves. Mechanisms leading to pulvinar turgor flux, particularly knowledge of the proteins involved, are not well-known. In this study we used two-dimensional gel electrophoresis and liquid chromatography-tandom mass spectrometry to separate and identify the proteins located in the soybean pulvinus. A total of 183 spots were separated and 195 proteins from 165 spots were identified and functionally analyzed using single enrichment analysis for gene ontology terms. The most significant terms were related to proton transport. Comparison with guard cell proteomes revealed similar significant processes but a greater number of pulvinus proteins are required for comparable analysis. To our knowledge, this is a novel report on the analysis of proteins found in soybean pulvinus. These findings provide a better understanding of the proteins required for turgor change in the pulvinus.

Variable selection for near-infrared spectrum modeling based on fast nondominated sorting genetic algorithm

2021 ◽  
pp. 1-13
Author(s):  
Hubin Liu ◽  
Chao Wang ◽  
Ge Wang ◽  
Weijie Xu ◽  
Longlian Zhao ◽  
...  
Keyword(s):  
Genetic Algorithm ◽  
Variable Selection ◽  
Infrared Spectrum ◽  
Near Infrared ◽  
Spectrum Modeling ◽  
Selection For ◽  
Nondominated Sorting ◽  
Near Infrared Spectrum

scholarly journals Opening of the Outer Membrane Protein Channel in Tripartite Efflux Pumps Is Induced by Interaction with the Membrane Fusion Partner

2010 ◽  
Vol 286 (7) ◽  
pp. 5484-5493 ◽  
Author(s):  
Thamarai K. Janganan ◽  
Li Zhang ◽  
Vassiliy N. Bavro ◽  
Dijana Matak-Vinkovic ◽  
Nelson P. Barrera ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Membrane Fusion ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Efflux Pumps ◽  
Fusion Partner ◽  
Protein Channel

scholarly journals Adición de proteínas del plasma seminal ovino durante la congelación del espermatozoide y efectos sobre su motilidad y viabilidad

2009 ◽  
Vol 10 (1) ◽  
pp. 51 ◽  
Author(s):  
Jaime Antonio Cardozo ◽  
Patricia Grasa ◽  
María Teresa Muiño B. ◽  
José Álvaro Cebrián P.
Keyword(s):  
Membrane Protein ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Two Dimensional ◽  
Plasma Séminal ◽  
Sperm Membrane ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

<p>Este estudio se adelantó para evaluar el efecto de la adición de proteínas del plasma seminal de cordero en la criopreservación sobre la motilidad e integridad de la membrana espermática, y los cambios en el perfil electroforético de las proteínas de la membrana espermática inducidos por la criopreservación. Se usaron eyaculados de ocho corderos adultos de la raza rasa aragonesa, se les determinó su viabilidad y motilidad espermáticas y posteriormente se sometieron a un procedimiento de congelación. Las proteínas se separaron por el método de electroforesis en geles de acrilamida en dos dimensiones. Se obtuvo un mejoramiento significativo (<em>p </em>&lt; 0,05) en la calidad del semen congelado, cuando se adicionaron proteínas del plasma seminal. El análisis bidimensional comparativo entre el semen fresco y el congelado evidenció la pérdida de 8 puntos de proteína en el espermatozoide descongelado. La concentración de un punto de proteína de membrana espermática, de bajo peso molecular (punto 2), fue más alta (<em>p </em>&lt; 0,05) en el espermatozoide descongelado al que se adicionaron proteínas del plasma seminal. Se encontraron correlaciones entre algunos puntos de proteína y la motilidad y viabilidad espermáticas, lo cual sugiere que pueden jugar papeles importantes en el mantenimiento de la integridad y funcionalidad del espermatozoide. Se puede concluir que la adición de proteínas del plasma seminal en la congelación mejora la integridad del espermatozoide descongelado, y que la criopreservación del semen de cordero produce variaciones en la composición de las proteínas de membrana.  </p><p> </p><p><strong>Effect of seminal plasma proteins at freezing on ram sperm motility and viability</strong>  </p><p>The aim of the study was to evaluate the cryoprotective effect of seminal plasma proteins on ram sperm motility, membrane integrity and the changes in the profile of ram sperm membrane proteins induced by cryopreservation. Fresh ejaculates from 8 mature Rasa aragonesa rams were used. Sperm motility and cell viability was assessed. The freezing procedure was based on the method described by Fiser <em>et al</em>. (1987). Proteins extracted from fresh and frozen-thawed semen were subjected to the Two-dimensional polyacrilamide gel electrophoresis. A significant improvement in the quality of frozenthawed sperm was obtained after addition of seminal plasma proteins (<em>p </em>&lt; 0.05). Comparative two-dimensional polyacrilamide gel electrophoresis analysis between fresh and frozen semen, either with or without seminal plasma proteins in the cryopreservation medium, revealed that eight protein spots were lost in frozen-thawed sperm. The concentration of one sperm membrane protein spot of low Mr (spot 2) was higher (<em>p </em>&lt; 0.05) in proteinadded frozen sperm. Correlations found between certain protein spots sperm motility and viability suggests that these proteins could play important roles in the maintenance of sperm integrity and functionality. In conclusion, the addition of seminal plasma proteins to freezing extender improved frozen-thawed ram sperm integrity quality and cryopreservation of ram semen produced variations in the sperm membrane protein composition. </p>

scholarly journals Nonlinear response of vibrational excitons: Simulating the two-dimensional infrared spectrum of liquid water

2009 ◽  
Vol 130 (20) ◽  
pp. 204110 ◽  
Author(s):  
A. Paarmann ◽  
T. Hayashi ◽  
S. Mukamel ◽  
R. J. D. Miller
Keyword(s):  
Infrared Spectrum ◽  
Liquid Water ◽  
Nonlinear Response ◽  
Two Dimensional

scholarly journals On the different sources of cooperativity in pH titrating sites of a membrane protein channel

2016 ◽  
Vol 39 (3) ◽  
Author(s):  
Antonio Alcaraz ◽  
María Queralt-Martín
Keyword(s):  
Membrane Protein ◽  
Protein Channel ◽  
Different Sources

Secondary dialkylammonium salt/crown ether [2]pseudorotaxanes as nanostructured platforms for proton transport

2018 ◽  
Vol 54 (58) ◽  
pp. 8092-8095 ◽  
Author(s):  
Lijie Li ◽  
Lipeng He ◽  
Binghua Wang ◽  
Peng Ge ◽  
Lei Jing ◽  
...  
Keyword(s):  
Crown Ether ◽  
Proton Conductivity ◽  
Proton Transport ◽  
Two Dimensional ◽  
Rational Control

Secondary dialkylammonium salt/crown ether [2]pseudorotaxanes can be confined into two-dimensional nanochannels, leading to remarkable enhancements and rational control of proton conductivity.

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