Elasticity-Based Patterning of Red Blood Cells on Undulated Lipid Membranes Supported on Porous Topographic Substrates†

2009 ◽  
Vol 113 (12) ◽  
pp. 3610-3614 ◽  
Author(s):  
Sang-Wook Lee ◽  
Cherlhyun Jeong ◽  
Sin-Doo Lee
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes

Membrane-permeabilizing activities of cyclic lipodepsipeptides, syringopeptin 22A and syringomycin E from Pseudomonas syringae pv. syringae in human red blood cells and in bilayer lipid membranes

2000 ◽  
Vol 52 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Gabriella Agner ◽  
Yuri A. Kaulin ◽  
Philip A. Gurnev ◽  
Zsofia Szabo ◽  
Ludmila V. Schagina ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Pseudomonas Syringae ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Syringomycin E ◽  
Bilayer Lipid ◽  
Human Red Blood Cells

Pore formation by the sea anemone cytolysin equinatoxin II in red blood cells and model lipid membranes

1993 ◽  
Vol 131 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Giovanna Belmonte ◽  
Cecilia Pederzolli ◽  
Peter Maček ◽  
Gianfranco Menestrina
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Pore Formation ◽  
Sea Anemone ◽  
Model Lipid Membranes ◽  
Equinatoxin Ii

scholarly journals ЗМІНИ ВЛАСТИВОСТЕЙ ЕРИТРОЦИТІВ У СОБАК

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 3-6
Author(s):  
M.V. Anforova ◽  
V.I. Golovakha ◽  
O.V. Piddubnyak ◽  
M.J. Tyshkivskyy
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Acid Resistance ◽  
Small Animals ◽  
Hemoglobin Content ◽  
Population Composition ◽  
Rapid Destruction ◽  
Healthy Dogs ◽  
Composition Changes

One of the most important indicators is the assessment of erythroсytopoezis is acid resistance of erythrocytes and their population composition. Changes in these parameters are the most studied in humans, cattle and horses. In small animals, including dogs, the issue is not highlighted enough. Therefore, the study of changes of erytroсytopoezis by using these tests is important. The study erythroсytopoezis parameters was performed in dogs of all ages (1.5–year, 3 – 6 years and 7 and older years of age). It was found that in clinically healthy dogs generall erythroсytopoezis indicators (total number of red blood cells, hemoglobin content, hematocrit value, the index «red» blood – МСН and МСV), regardless of age, did not significantly change. Yet in dogs older than 7 years there were found some changes on the the part of erythroсytopoezis. This was pointed by the changes of the population composition of red blood cells and their acid resistance. In particular, the animals showed an increased number of «old» populations of red blood cells and some changes of their hemolytic resistance. Particularly, the height of the erytrohram peak was 15% higher than in younger animals. The very peak was sharp, indicating the rapid destruction of the «young» red cells and degeneration of their lipid membranes. 

scholarly journals Effect of temperature on the formation and inactivation of syringomycin E pores in human red blood cells and bimolecular lipid membranes

2000 ◽  
Vol 1466 (1-2) ◽  
pp. 79-86 ◽  
Author(s):  
Gabriella Agner ◽  
Yuri A. Kaulin ◽  
Ludmila V. Schagina ◽  
Jon Y. Takemoto ◽  
Katalin Blasko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Effect Of Temperature ◽  
Syringomycin E ◽  
Human Red Blood Cells

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Phagocytosis of opsonized red blood cells by hepatocytes after gene transfer of chimeric Fc γR III Aα and γcDNA

2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Red Blood Cells ◽  
Blood Cells

Pathological alterations of cation movements in red blood cells

1972 ◽  
Vol 129 (2) ◽  
pp. 320-332 ◽  
Author(s):  
J. C. Parker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells
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