Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leishmania infantum Proliferation

Background: Leishmania infantum causes the most lethal form of Leishmaniasis: Visceral leishmaniasis. Current therapy for this disease is related to the development of drug-resistant species and toxicity. Trypanothione Reductase (LiTR), a validated target for the drug discovery process, is involved with parasites' thiol-redox metabolism. Objective: In this study, through Virtual Screening employing two distinct Natural Products Brazilian databases, we aimed to identify novel inhibitor scaffolds against LiTR. Results: Thus, the “top 10” LiTR-ligand energies have been selected and their interaction profiles into LiTR sites through the AuPosSOM server have been verified. Finally, Pred-hERG, Aggregator Advisor, FAF-DRUGS, pkCSM and DataWarrior were employed and their results allowed us to evaluate, respectively, the cardiotoxicity, aggregation capacity, presence of false-positive compounds (PAINS) and their toxicities. Conclusion: Three molecules that overcame the in silico pharmacokinetic analysis and have a good interaction with LiTR, were chosen to use in vitro assays hoping that our computational results reported here would aid the development of new anti-leishmanial compounds.

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Application of Furcellaran Nanocomposite Film as Packaging of Cheese

Polymers ◽

10.3390/polym13091428 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1428

Author(s):

Agnieszka Pluta-Kubica ◽

Ewelina Jamróz ◽

Gohar Khachatryan ◽

Adam Florkiewicz ◽

Pavel Kopel

Keyword(s):

Silver Nanoparticles ◽

Nanocomposite Film ◽

Microbiological Quality ◽

Inhibitory Effect ◽

Quality Properties ◽

In Situ Method ◽

And Migration ◽

Total Bacteria

There is a serious need to develop and test new biodegradable packaging which could at least partially replace petroleum-based materials. Therefore, the objective of this work was to examine the influence of the recently developed furcellaran nanocomposite film with silver nanoparticles (obtained by an in situ method) on the quality properties of two cheese varieties: a rennet-curd (gouda) and an acid-curd (quark) cheese. The water content, physicochemical properties, microbiological and organoleptic quality of cheese, and migration of silver nanoparticles were examined. Both the number of Lactococcus and total bacteria count did not differ during storage of gouda regardless of the packaging applied. The number of Lactococcus decreased in analogous quark samples. The use of the film slowed down and inhibited the growth of yeast in gouda and quark, respectively. An inhibitory effect of this film on mold count was also observed; however, only regarding gouda. The level of silver migration was found to be lower in quark than in gouda. The film improved the microbiological quality of cheeses during storage. Consequently, it is worth continuing research for the improvement of this film in order to enable its use in everyday life.

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The Synergistic Effect of Biosynthesized Silver Nanoparticles and Phage ZCSE2 as a Novel Approach to Combat Multidrug-Resistant Salmonella enterica

Antibiotics ◽

10.3390/antibiotics10060678 ◽

2021 ◽

Vol 10 (6) ◽

pp. 678

Author(s):

Abdallah S. Abdelsattar ◽

Rana Nofal ◽

Salsabil Makky ◽

Anan Safwat ◽

Amera Taha ◽

...

Keyword(s):

Silver Nanoparticles ◽

Color Change ◽

Antibacterial Properties ◽

Multidrug Resistant ◽

Inhibitory Effect ◽

Health Concern ◽

Resistant Bacteria ◽

Mortality And Morbidity ◽

Novel Approach ◽

Transmission Electron

The emergence and evolution of antibiotic-resistant bacteria is considered a public health concern. Salmonella is one of the most common pathogens that cause high mortality and morbidity rates in humans, animals, and poultry annually. In this work, we developed a combination of silver nanoparticles (AgNPs) with bacteriophage (phage) as an antimicrobial agent to control microbial growth. The synthesized AgNPs with propolis were characterized by testing their color change from transparent to deep brown by transmission electron microscopy (TEM) and Fourier-Transform Infrared Spectroscopy (FTIR). The phage ZCSE2 was found to be stable when combined with AgNPs. Both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated for AgNPs, phage, and their combination. The results indicated that MIC and MBC values were equal to 23 µg/mL against Salmonella bacteria at a concentration of 107 CFU/mL. The combination of 0.4× MIC from AgNPs and phage with Multiplicity of Infection (MOI) 0.1 showed an inhibitory effect. This combination of AgNPs and phage offers a prospect of nanoparticles with significantly enhanced antibacterial properties and therapeutic performance.

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Fungicidal effect of silver nanoparticles on toxigenic fungi in cocoa

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2016001200003 ◽

2016 ◽

Vol 51 (12) ◽

pp. 1929-1936 ◽

Cited By ~ 17

Author(s):

Raquel Villamizar-Gallardo ◽

Johann Faccelo Osma Cruz ◽

Oscar Orlando Ortíz-Rodriguez

Keyword(s):

Silver Nanoparticles ◽

Fusarium Solani ◽

Theobroma Cacao ◽

Mycelial Growth ◽

Culture Media ◽

Fungal Growth ◽

Inhibitory Effect ◽

Plant Tissues ◽

Inhibition Effect ◽

Toxigenic Fungi

Abstract: The objective of this work was to evaluate the microbicidal effect of silver nanoparticles (AgNPs) on potentially toxigenic fungi affecting cocoa (Theobroma cacao) crops. These fungi, isolated from diseased cocoa pods, were characterized phenotypically and genotypically. The microbicidal effect was assessed by measuring radial mycelial growth, in synthetic culture media, and at different AgNP concentrations in plant tissues. The inhibition effect was monitored in Petri dishes, and changes in fungal structures were observed through scanning electron microscopy. Two potentially toxigenic fungi were highly prevalent: Aspergillus flavus and Fusarium solani. The inhibition assays, performed in liquid and solid synthetic culture media, showed that AgNPs did not significantly affect the growth of these fungi, even at the highest concentration (100 ppm). By contrast, they showed a positive inhibitory effect in plant tissues, especially in the cortex, when infected with A. flavus, in which an 80 ppm dose completely inhibited fungal growth. However, once fungi have managed to penetrate inside the pods, their growth is unavoidable, and AgNP effect is reduced. On F. solani, the studied nanomaterial only induced some texture and pigmentation changes. The microbicidal effect of chemically synthesized silver nanoparticles is greater in plant tissues than in culture media.

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Modeled structure of trypanothione reductase of Leishmania infantum

BMB Reports ◽

10.5483/bmbrep.2008.41.6.444 ◽

2008 ◽

Vol 41 (6) ◽

pp. 444-447 ◽

Cited By ~ 27

Author(s):

Bishal K. Singh ◽

Nandini Sarkar ◽

M.V. Jagannadham ◽

Vikash K. Dubey

Keyword(s):

Leishmania Infantum ◽

Trypanothione Reductase ◽

Modeled Structure

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The Inhibition of wine microorganisms by silver nanoparticles

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1604 ◽

2021 ◽

Vol 15 ◽

pp. 995-1004

Author(s):

Aleš Vavřiník ◽

Kateřina Štůsková ◽

Adrian Alumbro ◽

Methusela Perrocha ◽

Lenka Sochorová ◽

...

Keyword(s):

Silver Nanoparticles ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Gluconobacter Oxydans ◽

Lactobacillus Brevis ◽

Inhibitory Effect ◽

Silver Particles ◽

Acetobacter Aceti ◽

Inhibition Zones

The presented work aimed to study the inhibition using nanoparticles produced by the green synthesis in selected acetic acid and lactic acid bacteria, which are related to viticulture. The degree of ability to eliminate silver particles produced by green syntheses was determined using the plate method on Petri dishes. This is done using two different approaches - the method of direct application of the solution to the surface of the inoculated medium (determination of inhibition zones) and the method of application using nanoparticles to the inoculated medium. Gluconobacter oxydans (CCM 3618) and Acetobacter aceti (CCM 3620T) were studied from acet acetic bacteria. The lactic acid bacteria were Lactobacillus brevis (CCM 1815) and Pediococcus damnosus (CCM 2465). The application of silver nanoparticles was always in concentrations of 0, 0.0625, 0.125, 0.25, 0.5, and 1 g.L-1. All applied concentrations of silver nanoparticles showed an inhibitory effect on the monitored microorganisms. Silver particles could be used in wine technology for their antibacterial effects, mainly to inhibit microorganisms during vinification, as a substitute for sulfur dioxide.

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A nano-platform for phenobarbital determination based on its inhibitory effect on the aggregation of silver nanoparticles/melamine system

Journal of Molecular Liquids ◽

10.1016/j.molliq.2020.113891 ◽

2020 ◽

Vol 316 ◽

pp. 113891

Author(s):

Samira Zadaliasghar ◽

Elaheh Rahimpour ◽

Maryam Khoubnasabjafari ◽

Mohammad Hossein Pournaghi-Azar ◽

Ali Nokhodchi ◽

...

Keyword(s):

Silver Nanoparticles ◽

Inhibitory Effect

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Antimicrobial and Substantivity Properties of Silver Nanoparticles against Oral Microbiomes Clinically Isolated from Young and Young-Adult Patients

Journal of Nanomaterials ◽

10.1155/2019/3205971 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14 ◽

Cited By ~ 2

Author(s):

León Francisco Espinosa-Cristóbal ◽

Carolina Holguín-Meráz ◽

Erasto Armando Zaragoza-Contreras ◽

Rita Elizabeth Martínez-Martínez ◽

Alejandro Donohue-Cornejo ◽

...

Keyword(s):

Silver Nanoparticles ◽

Young Adult ◽

Dental Caries ◽

Periodontal Disease ◽

Dental Plaque ◽

Bacterial Species ◽

Inhibitory Effect ◽

Oral Bacteria ◽

Oral Microbiome ◽

Oral Biofilms

The dental plaque is an oral microbiome hardly associated to be the etiological agent of dental caries and periodontal disease which are still considered serious health public problems. Silver nanoparticles (AgNPs) have demonstrated to have good antimicrobial properties affecting a wide variety of microorganisms, including oral bacteria; however, there is no scientific information that has evaluated the antimicrobial effect of AgNPs against clinical oral biofilms associated with dental caries and periodontal disease. The aim of this study was to determine the antimicrobial and substantivity effects of AgNPs in oral biofilms isolated clinically from patients with dental caries and periodontal disease. Sixty-seven young and young-adult subjects with dental caries and periodontal disease were clinically sampled through the collection of subgingival dental plaque. The inhibitory effect of AgNPs was performed with standard microbiological assays by triplicate using two sizes of particle. Polymerase chain reaction (PCR) assay was used to identify the presence of specific bacterial species. All AgNPs showed an inhibitory effect for all oral biofilms for any age and, generally, any gender (p>0.05); however, the effectiveness of the antimicrobial and substantivity effects was related to particle size, time, and gender (p<0.05). The identified microorganisms were S. mutans, S. sobrinus, S. sanguinis, S. gordonii, S. oralis, P. gingivalis, T. forsythia, and P. intermedia. The AgNPs could be considered as a potential antimicrobial agent for the control and prevention of dental caries and periodontal disease.

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Inhibition of Leishmania infantum trypanothione reductase by diaryl sulfide derivatives

Journal of Enzyme Inhibition and Medicinal Chemistry ◽

10.1080/14756366.2016.1250755 ◽

2017 ◽

Vol 32 (1) ◽

pp. 304-310 ◽

Cited By ~ 23

Author(s):

Francesco Saccoliti ◽

Gabriella Angiulli ◽

Giovanni Pupo ◽

Luca Pescatori ◽

Valentina Noemi Madia ◽

...

Keyword(s):

Leishmania Infantum ◽

Trypanothione Reductase ◽

Diaryl Sulfide

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Green Synthesis and Characterization of Silver Nanoparticles for Antimicrobial Activity Against Burn Wounds Contaminating Bacteria

International Journal of Nanoscience ◽

10.1142/s0219581x14500100 ◽

2014 ◽

Vol 13 (02) ◽

pp. 1450010

Author(s):

Anandini Rout ◽

Padan K. Jena ◽

Debasish Sahoo ◽

Umesh K. Parida ◽

Birendra K. Bindhani

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Inhibitory Effect ◽

Atmospheric Conditions ◽

Burn Wound ◽

X Ray ◽

Mueller Hinton ◽

Liquid Systems ◽

Mueller Hinton Agar

Silver nanoparticles ( AgNPs ) were prepared from the plant extract of N. arbor-tristis under atmospheric conditions through green synthesis and characterized by various physicochemical techniques like UV-Visible spectroscopy, IR Spectra, energy dispersive X-ray spectrometry (EDS), X-ray diffraction and transmission electron microscopy (TEM) and the results confirmed the synthesis of homogeneous and stable AgNPs by the plant extracts. The antimicrobial activity of AgNPs was investigated against most common bacteria found in burn wound Staphylococcus epidermidis and Pseudomonas aeruginosa. In these tests, Mueller Hinton agar plates were used with AgNPs of various concentrations, supplemented in liquid systems. P. aeruginosa was inhibited at the low concentration of AgNPs , whereas the growth-inhibitory effect on S. epidermidis was mild. These results suggest that AgNPs can be used as effective growth inhibitors of various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.

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