Quantitation of erbB-2 gene copy number in breast cancer by an improved polymerase chain reaction (PCR) technique, competitively differential PCR

1999 ◽  
Vol 58 (3) ◽  
pp. 211-215 ◽  
Author(s):  
Guoren Deng ◽  
Young S. Kim
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Chain Reaction ◽  
Polymerase Chain

Quantitation of Gene Copy Number and mRNA Using the Polymerase Chain Reaction

1992 ◽  
Vol 200 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M. Volkenandt ◽  
A. P. Dicker ◽  
D. Banerjee ◽  
R. Fanin ◽  
B. Schweitzer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Chain Reaction ◽  
Polymerase Chain

Competitive Polymerase Chain Reaction for the Quantification of N-myc Gene Copy Number in Neuroblastoma

Tumor Biology ◽  
1996 ◽  
Vol 17 (5) ◽  
pp. 262-270 ◽  
Author(s):  
Akira Inoue ◽  
Ziaul Hasan ◽  
Hiromichi Hemmi ◽  
Naotoshi Kanda ◽  
Yasuhide Hayashi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
I Gene

Determination of CYP2D6 gene copy number by multiplex polymerase chain reaction analysis

2009 ◽  
Vol 389 (1) ◽  
pp. 74-76 ◽  
Author(s):  
Luis J. Leandro-García ◽  
Susanna Leskelä ◽  
Cristina Montero-Conde ◽  
Iñigo Landa ◽  
Elena López-Jimenez ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Copy Number ◽  
Multiplex Polymerase Chain Reaction ◽  
Gene Copy Number ◽  
Polymerase Chain Reaction Analysis ◽  
Gene Copy ◽  
Chain Reaction ◽  
Cyp2d6 Gene ◽  
Polymerase Chain

Assessment of pfmdr 1 gene copy number by tandem competitive polymerase chain reaction

1997 ◽  
Vol 85 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Ric Price ◽  
Grant Robinson ◽  
Alan Brockman ◽  
Alan Cowman ◽  
Sanjeev Krishna
Keyword(s):  
Polymerase Chain Reaction ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Nongenotoxic ABCB1 activator tetraphenylphosphonium can contribute to doxorubicin resistance in MX-1 breast cancer cell line

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raimonda Kubiliute ◽  
Indre Januskeviciene ◽  
Ruta Urbanaviciute ◽  
Kristina Daniunaite ◽  
Monika Drobniene ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Protein Level ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Common Mechanism ◽  
Dna Hypomethylation ◽  
Mesenchymal Transition ◽  
Molecular Features

AbstractHyperactivation of ABC transporter ABCB1 and induction of epithelial–mesenchymal transition (EMT) are the most common mechanism of acquired cancer chemoresistance. This study describes possible mechanisms, that might contribute to upregulation of ABCB1 and synergistically boost the acquisition of doxorubicin (DOX) resistance in breast cancer MX-1 cell line. DOX resistance in MX-1 cell line was induced by a stepwise increase of drug concentration or by pretreatment of cells with an ABCB1 transporter activator tetraphenylphosphonium (TPP+) followed by DOX exposure. Transcriptome analysis of derived cells was performed by human gene expression microarrays and by quantitative PCR. Genetic and epigenetic mechanisms of ABCB1 regulation were evaluated by pyrosequencing and gene copy number variation analysis. Gradual activation of canonical EMT transcription factors with later activation of ABCB1 at the transcript level was observed in DOX-only treated cells, while TPP+ exposure induced considerable activation of ABCB1 at both, mRNA and protein level. The changes in ABCB1 mRNA and protein level were related to the promoter DNA hypomethylation and the increase in gene copy number. ABCB1-active cells were highly resistant to DOX and showed morphological and molecular features of EMT. The study suggests that nongenotoxic ABCB1 inducer can possibly accelerate development of DOX resistance.

scholarly journals Chromosomal Instability Selects Gene Copy-Number Variants Encoding Core Regulators of Proliferation in ER+ Breast Cancer

2014 ◽  
Vol 74 (17) ◽  
pp. 4853-4863 ◽  
Author(s):  
David Endesfelder ◽  
Rebecca A. Burrell ◽  
Nnennaya Kanu ◽  
Nicholas McGranahan ◽  
Mike Howell ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Copy Number ◽  
Chromosomal Instability ◽  
Copy Number Variants ◽  
Gene Copy Number ◽  
Gene Copy
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