Evaluation of consistency in quantification of gene copy number by real-time reverse transcription quantitative polymerase chain reaction and virus titer by plaque-forming assay for human respiratory syncytial virus

Abstract Nasopharyngeal (NP) swabs are generally used to detect respiratory syncytial virus (RSV) in infants. However, midturbinate (MT) swabs may provide comparable results. In this study, we enrolled hospitalized infants aged <24 months with RSV and collected NP and MT swabs. The resulting viral loads measured by real-time reverse-transcription quantitative polymerase chain reaction were similar. Most parents preferred MT swabs over NP swabs.

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Quantitation of erbB-2 gene copy number in breast cancer by an improved polymerase chain reaction (PCR) technique, competitively differential PCR

Breast Cancer Research and Treatment ◽

10.1023/a:1006367700783 ◽

1999 ◽

Vol 58 (3) ◽

pp. 211-215 ◽

Cited By ~ 3

Author(s):

Guoren Deng ◽

Young S. Kim

Keyword(s):

Breast Cancer ◽

Polymerase Chain Reaction ◽

Copy Number ◽

Gene Copy Number ◽

Gene Copy ◽

Chain Reaction ◽

Polymerase Chain

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Determination of CYP2D6 gene copy number by multiplex polymerase chain reaction analysis

Analytical Biochemistry ◽

10.1016/j.ab.2009.03.021 ◽

2009 ◽

Vol 389 (1) ◽

pp. 74-76 ◽

Cited By ~ 11

Author(s):

Luis J. Leandro-García ◽

Susanna Leskelä ◽

Cristina Montero-Conde ◽

Iñigo Landa ◽

Elena López-Jimenez ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Copy Number ◽

Multiplex Polymerase Chain Reaction ◽

Gene Copy Number ◽

Polymerase Chain Reaction Analysis ◽

Gene Copy ◽

Chain Reaction ◽

Cyp2d6 Gene ◽

Polymerase Chain

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Assessment of pfmdr 1 gene copy number by tandem competitive polymerase chain reaction

Molecular and Biochemical Parasitology ◽

10.1016/s0166-6851(96)02822-8 ◽

1997 ◽

Vol 85 (2) ◽

pp. 161-169 ◽

Cited By ~ 23

Author(s):

Ric Price ◽

Grant Robinson ◽

Alan Brockman ◽

Alan Cowman ◽

Sanjeev Krishna

Keyword(s):

Polymerase Chain Reaction ◽

Copy Number ◽

Gene Copy Number ◽

Gene Copy ◽

Chain Reaction ◽

Competitive Polymerase Chain Reaction ◽

Polymerase Chain

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Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

Annals of Otology Rhinology & Laryngology ◽

10.1177/0003489492101s1003 ◽

1992 ◽

Vol 101 (10_suppl) ◽

pp. 7-10 ◽

Cited By ~ 19

Author(s):

Yoshitaka Okamoto ◽

Kazuo Kudo ◽

Koji Shirotori ◽

Misao Nakazawa ◽

Eiko Ito ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Respiratory Syncytial Virus ◽

Otitis Media ◽

Otitis Media With Effusion ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Rsv Infection ◽

Polymerase Chain ◽

Syncytial Virus ◽

Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

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Respiratory Pathogens in Children 1 Month to 5 Years of Age Presenting With Undifferentiated Acute Respiratory Distress in 2 District-Level Hospitals in Ghana

Journal of the Pediatric Infectious Diseases Society ◽

10.1093/jpids/piy090 ◽

2018 ◽

Vol 8 (4) ◽

pp. 361-364

Author(s):

Patrick T Wilson ◽

Frank Baiden ◽

Joshua C Brooks ◽

Katie M Giessler ◽

Gavin Apio ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Respiratory Syncytial Virus ◽

Respiratory Distress ◽

Polymerase Chain Reaction Assay ◽

Rainy Season ◽

Dry Season ◽

Respiratory Pathogens ◽

Chain Reaction ◽

Polymerase Chain ◽

Syncytial Virus

Abstract Ghanaian children (2176) aged <5 years who presented with undifferentiated acute respiratory distress were tested for respiratory pathogens using a BioFire FilmArray polymerase chain reaction assay. Rhinovirus and/or enterovirus was detected in 36% of the assays, respiratory syncytial virus in 11%, and parainfluenza in 7%. Respiratory syncytial virus and metapneumovirus were detected more frequently in the rainy season than in the dry season.

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The Distribution and Detection of Grapevine red blotch virus in its Host Depend on Time of Sampling and Tissue Type

Plant Disease ◽

10.1094/pdis-03-18-0450-re ◽

2018 ◽

Vol 102 (11) ◽

pp. 2187-2193 ◽

Cited By ~ 5

Author(s):

Felicia J. Setiono ◽

Debotri Chatterjee ◽

Marc Fuchs ◽

Keith L. Perry ◽

Jeremy R. Thompson

Keyword(s):

Polymerase Chain Reaction ◽

Seasonal Variations ◽

Quantitative Polymerase Chain Reaction ◽

Virus Titer ◽

Tissue Type ◽

Chain Reaction ◽

Variable Distribution ◽

Qpcr Method ◽

Polymerase Chain ◽

Virus Distribution

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch, an emerging disease that affects cultivated grapevine such as Vitis vinifera. The ability to detect viruses in grapevine is often hindered by low virus titers compounded by a variable distribution in the plant and seasonal variations. In order to examine these two variables in relation to GRBV, we developed a quantitative polymerase chain reaction (qPCR) method that incorporates both internal and external references to enhance assay robustness. In greenhouse-grown vines infected with GRBV, qPCR identified highest virus titers in the petioles of fully expanded leaves and significantly reduced levels of virus in the shoot extremities. In vineyard-grown vines infected with GRBV, the virus titer in July and October 2016 followed a pattern similar to that found for the greenhouse-grown plants but, most strikingly, close to half (44%) of the samples analyzed in June 2015 tested negative for infection. The technique presented and results obtained highlight the variability of virus distribution in its host and provide a useful guide for selecting the best tissues for optimal GRBV diagnosis.

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