Anticoagulant Activity of Fucoidan from Brown Algae Fucus evanescens of the Okhotsk Sea

2003 ◽  
Vol 136 (5) ◽  
pp. 471-473 ◽  
Author(s):  
T. A. Kuznetsova ◽  
N. N. Besednova ◽  
A. N. Mamaev ◽  
A. P. Momot ◽  
N. M. Shevchenko ◽  
...  
2007 ◽  
Vol 143 (6) ◽  
pp. 730-732 ◽  
Author(s):  
T. V. Alekseyenko ◽  
S. Ya. Zhanayeva ◽  
A. A. Venediktova ◽  
T. N. Zvyagintseva ◽  
T. A. Kuznetsova ◽  
...  

Author(s):  
Natalya V. Krylova ◽  
Svetlana P. Ermakova ◽  
Vyacheslav F. Lavrov ◽  
Irina A. Leneva ◽  
Galina G. Kompanets ◽  
...  

2020 ◽  
Vol 65 (5-6) ◽  
pp. 3-10
Author(s):  
S. R. Khil'chenko ◽  
T. S. Zaporozhets ◽  
T. N. Zvyagintseva ◽  
N. M. Shevchenko ◽  
N. N. Besednov

Fucoidans, sulfated polysaccharides extracted from brown algae (Phaeophyceae), have a wide spectrum of bioactivity. Studies of molecular structures of fucoidans and deciphering of molecular elements' impact on their biological activities are at their active stage. The article shows the role of sulfates and acetyl groups in fucoidan isolated from Fucus evanescens in proinflammatory cytokines production by human heparinized unfractionated peripheral blood cells. Material and Methods. The cells were incubated with native fucoidan (N) and its deacetylated (deA), partially desulfated (deS), and both deacetylated and partially desulfated (deAdeS) derivatives (100 μg/mL). Cytokine concentrations were determined in cell supernatants by ELISA in a 'sandwich' modification with commercial kits. Results. Incubation with N fucoidan led to an increase of IL-6, TNF-α, IL-8 levels in supernatants. Partial removal of sulfate groups cancelled or decreased stimulating effect for IL-6, TNF-α, cytokines, but not for IL-8. deAc fucoidan action was comparable with N polysaccharide. Native polysaccharide and its chemically modified derivatives did not change IFN-γ и IL-10 cytokine production. Conclusion. The obtained results suggest that sulfates have a significant role in cytokine-producing properties of fucoidan extracted from brown algae F.evanescens.


Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1208-1208 ◽  
Author(s):  
Michael Dockal ◽  
Susanne Till ◽  
Sabine Knappe ◽  
Hartmut J. Ehrlich ◽  
Friedrich Scheiflinger

Abstract Abstract 1208 Fucoidans are sulfated polysaccharides which are extracted from brown seaweeds and echinoderms and have a wide variety of biological activities. Described as non-anticoagulant polysaccharides (NASPs), they have been demonstrated to improve clotting in FVIII- and FIX-deficient plasma (Liu et al., 2006), making them good candidates for hemophilia treatment. However, fucoidans have also been extensively studied for their anticoagulant effects (Pereira et al., 1999), which usually occur at much higher concentrations than the procoagulant activity. This opens a large procoagulant window where procoagulant activities exceed anticoagulant effects. When analyzed by a global hemostatic thrombin generation assay in hemophilia plasma the onset of procoagulant activity is observed at concentrations as low as 0.01 μg/mL with optimal activity at about 1 μg/mL. Reversal of procoagulant activity is seen at concentrations higher than 10 μg/mL. Fucoidans and other sulfated polysaccharides activate different anticoagulant mechanisms depending on their structural properties. Branched fucoidans extracted from brown algae have been shown to directly inhibit thrombin, while linear fucoidan from echinoderms activates antithrombin III (ATIII) or heparin cofactor II (HCII)-mediated thrombin inhibition (Pereira et al., 1999). Sulfated galactans also have serpin-dependent and -independent anticoagulant activities (Glauser et al., 2009). In this study we analyzed fucoidans from several brown algae species for their anticoagulant properties and mode of action to identify the candidate with the best procoagulant and lowest anticoagulant activity. NASPs from several brown algae species including L. japonica (L.j.), F. vesiculosus (F.v.), and U. pinnatifida (U.p.) showed different anticoagulant activities in an activated partial thromboplastin time (aPTT) assay. U.p. fucoidan was about twice as anticoagulant as the other fucoidan preparations, increasing clotting time by 50% at a concentration of 4 μg/mL. In addition, NASPs were analyzed in ATIII- and HCII-thrombin model assays. L.j. fucoidan activated ATIII-mediated thrombin inhibition, whereas the other fucoidans showed no effect on ATIII. Fucoidans from L.j. and F.v. had a direct effect on thrombin, starting at about 10 μg/mL. By contrast, U.p. fucoidan did not directly affect thrombin. However, all preparations increased HCII-mediated thrombin inhibition at concentrations below 1 μg/mL. This suggests that HCII is the main target for the anticoagulant activity of fucoidans. Nevertheless, we observed substantial differences between the fucoidan candidates which will be correlated to structural properties. Our work describes the assessment of anticoagulant activities of a variety of fucoidan species to better understand their intertwined pro- and anticoagulant effects. This provides important mechanistic insights for the development of hemophilia therapies. Disclosures: Dockal: Baxter Innovations GmbH: Employment. Till:Baxter Innovations GmbH: Employment. Knappe:Baxter Innovations GmbH: Employment. Ehrlich:Baxter Innovations GmbH: Employment. Scheiflinger:Baxter BioScience: Employment.


2002 ◽  
Vol 43 (2) ◽  
pp. 242-249 ◽  
Author(s):  
E.P. Ivanova ◽  
I.Yu. Bakunina ◽  
T. Sawabe ◽  
K. Hayashi ◽  
Y.V. Alexeeva ◽  
...  

Microbiology ◽  
2012 ◽  
Vol 81 (3) ◽  
pp. 367-372 ◽  
Author(s):  
S. P. Ermakova ◽  
E. P. Ivanova ◽  
I. Yu. Bakunina ◽  
V. V. Mikhailov ◽  
T. N. Zvyagintseva

1966 ◽  
Vol 44 (1) ◽  
pp. 89-93 ◽  
Author(s):  
T. Bisalputra

The protoplasmic continuity in the meristoderm and outer cortex of the blades of Egregia menziesii and Fucus evanescens is demonstrated by electron micros copy. Groups of small pores of the order of 37.5 mμ in diameter perforate the walls in these regions. Traversing the pores are protoplasmic strands connecting the adjacent protoplasts. It is concluded that these pores are plasmodesmata which occur in primary pit fields. Endoplasmic reticulum is not found within the plasmodesma.


2012 ◽  
Vol 358 ◽  
pp. 78-81 ◽  
Author(s):  
Stanislav D. Anastyuk ◽  
Natalia M. Shevchenko ◽  
Pavel S. Dmitrenok ◽  
Tatyana N. Zvyagintseva

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