The identification of inducible cytoplasmic/nuclear carbohydrate-binding proteins urges to develop novel concepts about the role of plant lectins

2003 ◽  
Vol 20 (7/8) ◽  
pp. 449-460 ◽  
Author(s):  
Els J.M. Van Damme ◽  
Nausicaä Lannoo ◽  
Elke Fouquaert ◽  
Willy J. Peumans
Keyword(s):  
Binding Proteins ◽  
Carbohydrate Binding ◽  
Plant Lectins ◽  
Carbohydrate Binding Proteins

Inhibition of sperm-egg fusion in the hamster and mouse by carbohydrates

Zygote ◽  
1994 ◽  
Vol 2 (3) ◽  
pp. 253-262 ◽  
Author(s):  
Ruben H. Ponce ◽  
Umbert A. Urch ◽  
Ryuzo Yanagimachi
Keyword(s):  
Extracellular Matrix ◽  
Plasma Membrane ◽  
Zona Pellucida ◽  
Binding Proteins ◽  
Toxic Effects ◽  
Carbohydrate Binding ◽  
Dextran Sulphate ◽  
Membrane Glycoproteins ◽  
Carbohydrate Binding Proteins

SummaryAfter spermatozoa bind to and penetrate the extracellular matrix of the egg, the zona pellucida, they adhere to and fuse with the plasma membrane of the egg. Since sperm–egg fusion may involve membrane glycoproteins and/or carbohydrate binding proteins, we sought to test this hypothesis by challenging sperm–egg fusion in hamster and in mouse with added carbohydrates. In this study, a number of carbohydrate and glycoconjugates were examined for their ability to inhibit sperm–eggfusion. In the hamster, D(+)-glucosamine, D(+)-galactosamine, albumin-bovine-glucosamide and-galactosamide, fucoidan and dextran sulphate inhibited the fusion of spermatozoa with zona-free eggs. The same effects were seen in the mouse, except for the toxic effects of D(+)-galactosamine. These facts suggest a role of carbohydrate binding proteins or glycoproteins in the fertilisation process at the level of binding to and fusing with the oolemma.

scholarly journals Effects of Plant Lectins on Human Erythrocyte Agglutination

2016 ◽  
Vol 17 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Nadja Zubcevic ◽  
Suljevic Damir ◽  
Muhamed Focak ◽  
Dunja Rukavina
Keyword(s):  
Plant Species ◽  
Plant Extracts ◽  
Human Erythrocyte ◽  
Binding Proteins ◽  
Carbohydrate Binding ◽  
Plant Lectins ◽  
Blood Types ◽  
The Family ◽  
Carbohydrate Binding Proteins

AbstractPlant lectins are carbohydrate binding proteins or phytohaemagglutinins present in most plants, especially seeds and tubers, which include cereals, potatoes and beans. Lectins have great significance in the diet because of their involvement in gastrointestinal difficulties and erythrocyte agglutination. Blood agglutination activity against A, B, AB and O groups was shown after exposing blood to extracts obtained from 55% of tested plants, while in 45% of plants, agglutination was absent. The results of our study have shown that in humans, 40% of plant extracts exhibited activity against A, 40% of plant extracts exhibited activity against B, and 50% of plant extracts exhibited activity against AB and O groups in humans. The concentration of plant lectins depends on the part of the plant. Lectins from the seeds of certain plants cause the greatest percentage of erythrocyte agglutination, while the lowest agglutination was caused by plant bulbs and leaves. However, lectins derived from all plant species of the family Fabaceae agglutinated erythrocytes of all blood types to some extent.

Generation of H2O2 by Human Neutrophils and Changes of Cytosolic Ca2+ and pH of Rat Thymocytes in Response to Galactoside-Binding Proteins (Lectins or Immunoglobulins)

1997 ◽  
Vol 17 (2) ◽  
pp. 219-230 ◽  
Author(s):  
A. V. Timoshenko ◽  
S. André ◽  
H. Kaltner ◽  
X. Dong ◽  
H.-J. Gabius
Keyword(s):  
Binding Proteins ◽  
Biological Activities ◽  
Viscum Album ◽  
Human Neutrophils ◽  
Carbohydrate Binding ◽  
Carbohydrate Specificity ◽  
Plant Lectins ◽  
Endogenous Lectins ◽  
Carbohydrate Binding Proteins ◽  
Ph Increase

In contrast to plant agglutinins, biological activities of animal/human lectins are not well defined yet. Testing a panel of seven mammalian carbohydrate-binding proteins we have found that the dimeric lectin from chicken liver (CL-16) was a stimulator of H2O2 release from human neutrophils as well as effector for induction of cytosolic Ca2+ and pH increase in rat thymocytes. Activity of this lectin was comparable to potent galactoside-specific plant lectins such as Viscum album L. agglutinin. The activities of the tested plant lectins depended significantly on their nominal carbohydrate specificity as well as on the source. The results indicate that endogenous lectins may be involved in the regulation of neutrophil and lymphocyte functions by elicitation of selective biosignaling reactions.

Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts

2014 ◽  
Vol 2 (11) ◽  
pp. 1490-1498 ◽  
Author(s):  
Sarah-Jane Richards ◽  
Elizabeth Fullam ◽  
Gurdyal S. Besra ◽  
Matthew I. Gibson
Keyword(s):  
Gold Nanoparticles ◽  
Differential Expression ◽  
Polymer Coating ◽  
Binding Proteins ◽  
Carbohydrate Binding ◽  
Bacterial Strains ◽  
Carbohydrate Binding Proteins ◽  
The Impact

Gold nanoparticles are employed to discriminate between bacterial strains based on their differential expression of carbohydrate-binding proteins. The role of carbohydrate presentation on sensory output is studied.

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