<p>Intact accumulating peatlands are a globally important terrestrial carbon sink. Climate change and agricultural drainage are degrading these ecosystems, and through increases in aerobic decomposition, shifting their C balance from sink to source. To argue the effectiveness of restoration activities (such as rewetting), techniques are needed that clearly show differences between drained and natural (or drained and rewetted) peatlands. Because these changes are not always macroscopically visible, molecular analysis methods are especially needed to distinguish between ecosystems experiencing net pet growth (sequestering carbon), and those where aerobic decomposition is still a primary driving mechanism. Molecular biomarkers are a useful way to use chemical composition to distinguish these mechanisms.</p><p>This study aimed to compare differences in chemical composition with depth between two peatland sites from a large ombrotrophic mire in Lakkasuo Finland &#8211; one natural and one drained. To characterize these chemical shifts, pyrolysis gas chromatography mass spectrometry was used to track changes in relative abundance of various molecular biomarkers and compound classes (ie., aromatics, Sphagnum phenols, lignin, N-containing compounds, n-alkanes, etc.) with depth across both sites. Three replicate cores per site were collected, allowing for statistical evaluation of the relative abundances of these compounds. Using radiocarbon dating at three depths per core, the drained and natural sites were also matched by age for reference purposes. Significant differences were found for the Sphagnum-specific biomarker, p-isopropenylphenol, aromatics, and lignin, to the approximate current depth of the drained peatland water table. Higher phenolic compound class abundance indicated inhibited aerobic decomposition in the natural cores. An increasing trend in lignin biomarker relative abundance with depth was observed in the natural site, despite the identification of comparatively fewer vascular plants during the macroscopic analysis. Rather than a higher abundance of palaeo-ecological vascular plants, this trend is considered to be an indicator of preferential preservation of lignin compounds with anaerobic conditions. Below the depth of the water table, the relative abundances of most biomarkers stabilized, indicating the existance of similar environmental conditions in both sites prior to drainage. These data were compared and are in agreement with findings from elemental analysis (CHNO) and bulk isotopic (<sup>13</sup>C and <sup>15</sup>N) data measured on the same cores. Collectively, these data suggest that observed shifts in chemical composition in the natural and drained cores reflect the effect of different hydrological conditions between the two sites.</p>
Methane transport and emissions from soil as affected by water table and vascular plants
