Intracellular localization of sucrose-phosphate phosphatase in storage cells

1995 ◽  
Vol 95 (4) ◽  
pp. 559-562
Author(s):  
Ed Echeverria
Keyword(s):  
Intracellular Localization ◽  
Sucrose Phosphate Phosphatase ◽  
Storage Cells ◽  
Sucrose Phosphate

scholarly journals Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Fernando Henrique Correr ◽  
Guilherme Kenichi Hosaka ◽  
Fernanda Zatti Barreto ◽  
Isabella Barros Valadão ◽  
Thiago Willian Almeida Balsalobre ◽  
...  
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Carbon Partitioning ◽  
Biomass Composition ◽  
Energy Crop ◽  
Complex Nature ◽  
High Biomass ◽  
Sucrose Phosphate Phosphatase ◽  
Source Tissue ◽  
Industrial Yield ◽  
Sucrose Phosphate

Abstract Background The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability. Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number. To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition. Results First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq. We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning. We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes. Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis. However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group. Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group. Conclusions Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups. Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.

scholarly journals Sucrose Transport into Citrus Juice Cells: Evidence for an Endocytic Transport System

2005 ◽  
Vol 130 (2) ◽  
pp. 269-274 ◽  
Author(s):  
Ed Etxeberria ◽  
Pedro Gonzalez ◽  
Javier Pozueta-Romero
Keyword(s):  
Developmental Stages ◽  
Sucrose Phosphate Synthase ◽  
Sink Strength ◽  
High Definition ◽  
Sucrose Transport ◽  
Root Cells ◽  
Sucrose Metabolizing Enzymes ◽  
Cytoplasmic Vesicles ◽  
Sucrose Phosphate Phosphatase ◽  
Sucrose Phosphate

To investigate the mechanisms of sucrose transport and its accumulation into `Murcott' mandarin (Citrus reticulata Blanco) fruit, developmental changes in determinants of sink strength such as sucrose metabolizing enzymes, and sucrose transport across both plasmalemma and tonoplast were analyzed. Concurrently with sucrose levels, sucrose synthase, sucrose phosphate synthase and sucrose phosphate phosphatase increased throughout fruit development. Plasmalemma and tonoplast vesicles isolated from fruit collected at different developmental stages were analyzed for their transport capabilities. Sucrose uptake into energized plasmalemma vesicles was abolished by gramicidin, which is in accordance with the presence of an active symport mechanism of sucrose transport from the apoplast into the cytosol. Unexpectedly, tonoplast vesicles were shown to lack active transport mechanism of sucrose into the vacuole. More importantly, however, and in conformity with recent findings showing the occurrence of an endocytic mechanism of ion uptake in maize (Zea mays L.) root cells, citrus (Citrus L.) juice cells were shown to incorporate membrane impermeable dyes into their vacuoles in the presence of sucrose. High definition confocal microscopy revealed the co-localization of membrane impermeable markers in cytoplasmic vesicles and the formation of vesicles at the plasmalemma. The data provide evidence for an endocytic system of transport that allows direct incorporation of sucrose from the apoplast to the vacuole bypassing both the plasmalemma and tonoplast.

scholarly journals Characterization of the Sucrose Phosphate Phosphatase (SPP) Isoforms from Arabidopsis thaliana and Role of the S6PPc Domain in Dimerization

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0166308 ◽  
Author(s):  
Tomás Albi ◽  
M. Teresa Ruiz ◽  
Pedro de los Reyes ◽  
Federico Valverde ◽  
José M. Romero
Keyword(s):  
Arabidopsis Thaliana ◽  
Sucrose Phosphate Phosphatase ◽  
Sucrose Phosphate

scholarly journals Differential Expression in Leaves of Saccharum Genotypes Contrasting in Biomass Production Provides Evidence of Genes Involved in Carbon Partitioning

2020 ◽  
Author(s):  
Fernando Henrique Correr ◽  
Guilherme Kenichi Hosaka ◽  
Fernanda Zatti Barreto ◽  
Isabella Barros Valadão ◽  
Thiago Willian Almeida Balsalobre ◽  
...  
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Carbon Partitioning ◽  
Biomass Composition ◽  
Energy Crop ◽  
Rna Seq ◽  
High Biomass ◽  
Sucrose Phosphate Phosphatase ◽  
Source Tissue ◽  
Industrial Yield ◽  
Sucrose Phosphate

Abstract Background The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability. Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number. To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition. Results First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-SEq. We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning. We found evidence that the variation between these groups may be partly due to the expansion of the Saccharum genomes by differential transposition and defense related genes. Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis. However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group. Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group. Conclusions Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups. Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.

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