Effect in vitro and in vivo of a rat anti-CD2 monoclonal antibody (LO-CD2b) on pig-to-baboon xenogeneic cellular (T and natural killer cells) immune response

2001 ◽  
Vol 8 (3) ◽  
pp. 193-201 ◽  
Author(s):  
Jean-Paul Dehoux ◽  
Bernardo De La Parra ◽  
Dominique Latinne ◽  
Hervé Bazin ◽  
Pierre Gianello
Keyword(s):  
Monoclonal Antibody ◽  
Immune Response ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Killer Cells

scholarly journals Enhancement of in vitro beta-thalassemic and normal hematopoiesis by a noncytotoxic monoclonal antibody, 9.1C3: evidence for negative regulation of hematopoiesis by monocytes and natural killer cells

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1124-1133
Author(s):  
G Kannourakis ◽  
GR Johnson ◽  
CG Begley ◽  
JA Werkmeister ◽  
GF Burns
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Colony Formation ◽  
Mononuclear Cells ◽  
Regulatory Control ◽  
Beta Thalassemia ◽  
Killer Cells

The enhancement of in vitro human hematopoiesis by the addition of a noncytotoxic monoclonal antibody, 9.1C3, is described. Enhancement of all aspects of in vitro hematopoiesis was observed on addition of 9.1C3 antibody to cultures of mononuclear cells from normal bone marrow, cord blood, and peripheral blood from beta-thalassemia major patients. In cultures with no exogenous colony-stimulating factor (CSF), the addition of 9.1C3 resulted in a two- to eightfold increase in nonerythroid colony formation. Similarly, for cultures maximally stimulated with CSF, the addition of 9.1C3 antibody resulted in a one- to fourfold increase in colony formation. These effects were abrogated by the removal of either adherent, Leu-M3+ or Leu-7+ cells. Colony- forming cells were shown to be present among the 9.1C3-negative cells when mononuclear cells were sorted by flow cytometry. Media conditioned in the presence of 9.1C3 and mononuclear cells were able to enhance colony formation in vitro for normal nonadherent bone marrow cells beyond that achieved with supramaximal amounts of human placental- conditioned medium and erythropoietin. The data suggest that natural killer cells interact with monocytes to exert a negative regulatory control on in vitro granulopoiesis and erythropoiesis. Consequently, the number of progenitor and multipotential cells in cultures of unfractionated cell populations may be greatly underestimated.

scholarly journals GMP-Compliant, Large-Scale Expanded Allogeneic Natural Killer Cells Have Potent Cytolytic Activity against Cancer Cells In Vitro and In Vivo

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53611 ◽  
Author(s):  
Okjae Lim ◽  
Yuna Lee ◽  
Hyejin Chung ◽  
Jung Hyun Her ◽  
Sang Mi Kang ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Cancer Cells ◽  
Natural Killer ◽  
Large Scale ◽  
Cytolytic Activity ◽  
Killer Cells
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