Chemosensory cue conditioning with stimulants in a Caenorhabditis elegans animal model of addiction.

AbstractLong non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among the intestinal lncRNAs, linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment. Among these 8 intestinal lncRNAs, RNAi knockdown of linc-2 or linc-61 induced a susceptibility to toxicity of simulated microgravity, whereas RNAi knockdown of linc-13, linc-14, or linc-50 induced a resistance to toxicity of simulated microgravity. In simulated microgravity treated nematodes, linc-50 potentially binds to three transcriptional factors (DAF-16, SKN-1, and HLH-30). RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Therefore, our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes.

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An optimized assay for early and rapid assessment of behavioral alterations using Caenorhabditis elegans as an alternate animal model

Science Archives ◽

10.47587/sa.2021.2109 ◽

2021 ◽

Vol 02 (01) ◽

pp. 56-61

Author(s):

Sarita Mishra ◽

Rakhi Agarwal

Keyword(s):

Animal Model ◽

Caenorhabditis Elegans ◽

Rapid Assessment ◽

Behavioral Alterations

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Assessment of the effects of seasonality on the ecotoxicity induced by the particulate matter using the animal model Caenorhabditis elegans

Chemosphere ◽

10.1016/j.chemosphere.2021.132886 ◽

2021 ◽

pp. 132886

Author(s):

Maria Fernanda Cáceres Quijano ◽

Joaquim de Paula Ribeiro ◽

Marcelo Estrella Josende ◽

Eduarda Santa-Helena ◽

Anna De Falco ◽

...

Keyword(s):

Animal Model ◽

Particulate Matter ◽

Caenorhabditis Elegans

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Caenorhabditis elegans: nature and nurture gift to nematode parasitologists

Parasitology ◽

10.1017/s0031182017002165 ◽

2017 ◽

Vol 145 (8) ◽

pp. 979-987 ◽

Cited By ~ 5

Author(s):

Gustavo Salinas ◽

Gastón Risi

Keyword(s):

Animal Model ◽

Caenorhabditis Elegans ◽

Parasitic Nematode ◽

Model Organism ◽

Parasitic Nematodes ◽

Free Living ◽

C Elegans ◽

Free Living Nematode ◽

Basic Biology ◽

Nature And Nurture

AbstractThe free-living nematode Caenorhabditis elegans is the simplest animal model organism to work with. Substantial knowledge and tools have accumulated over 50 years of C. elegans research. The use of C. elegans relating to parasitic nematodes from a basic biology standpoint or an applied perspective has increased in recent years. The wealth of information gained on the model organism, the use of the powerful approaches and technologies that have advanced C. elegans research to parasitic nematodes and the enormous success of the omics fields have contributed to bridge the divide between C. elegans and parasite nematode researchers. We review key fields, such as genomics, drug discovery and genetics, where C. elegans and nematode parasite research have convened. We advocate the use of C. elegans as a model to study helminth metabolism, a neglected area ready to advance. How emerging technologies being used in C. elegans can pave the way for parasitic nematode research is discussed.

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