scholarly journals Histochemical Evidence of Increased Activity of Hydrolytic Enzymes in the Cells of Old Animals

Nature ◽  
1957 ◽  
Vol 179 (4557) ◽  
pp. 472-472 ◽  
Author(s):  
GEOFFREY H. BOURNE
Keyword(s):  
Hydrolytic Enzymes ◽  
Histochemical Evidence ◽  
Increased Activity

Carbon nutrition and hydrolytic and cellulolytic activities in the ectomycorrhizal fungus Pisolithus tinctorius

1993 ◽  
Vol 39 (5) ◽  
pp. 529-535 ◽  
Author(s):  
Weiguo Cao ◽  
Don L. Crawford
Keyword(s):  
Hydrolytic Enzymes ◽  
Ectomycorrhizal Fungus ◽  
Isozyme Pattern ◽  
Pisolithus Tinctorius ◽  
Cellulolytic Enzymes ◽  
Cellulolytic Activity ◽  
Gradient Gel Electrophoresis ◽  
Carbon Nutrition ◽  
High Concentrations ◽  
Increased Activity

Four strains of an ectomycorrhizal fungus, Pisolithus tinctorius, were investigated for carbon nutrition, and for production of hydrolytic and cellulolytic enzymes. Glucose, mannose, and cellobiose supported rapid mycelial growth of all four strains. Fructose was utilized by two strains, SMF and S359. Of the 10 hydrolytic enzymes examined, acid phosphatase, acid α-galactosidase, acid esterase, and acid β-glucosidase were found in all four strains. β-Galactosidase was only observed in strain S359. α-Mannosidase, β-mannosidase, α-glucosidase, β-xylosidase, and proteinase were not detected in any of the four strains. Isozyme patterns of β-glucosidase and esterase in the four strains were compared by activity staining after native gradient gel electrophoresis. The isozyme pattern of β-glucosidase showed three major forms in all four strains. In addition, two more isoforms were found in strain S370. All strains shared two esterase bands, while strain S370 had three more isoforms. Study on strain SMF indicated that acid β-glucosidase was expressed constitutively, with increased activity in cellobiose-containing media. Under nitrogen-limiting conditions, a low level of endoglucanase and exoglucanase activity was observed in strains SMF and S359. Further study on S359 showed that high concentrations of nitrogen repressed the cellulolytic activity. When cellobiose served as carbon source, higher cellulolytic activity was observed. Cellulose did not induce higher activity.Key words: Pisolithus, ectomycorrhizal, β-glucosidase, hydrolytic enzymes, cellulolytic enzymes.

scholarly journals PROSPECTING CYANOBACTERIA-FORTIFIED COMPOSTS AS PLANT GROWTH PROMOTING AND BIOCONTROL AGENTS IN COTTON

2014 ◽  
Vol 51 (1) ◽  
pp. 42-65 ◽  
Author(s):  
RADHA PRASANNA ◽  
SANTOSH BABU ◽  
NGANGOM BIDYARANI ◽  
ARUN KUMAR ◽  
SODIMALLA TRIVENI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Management Strategies ◽  
Hydrolytic Enzymes ◽  
Biocontrol Agents ◽  
Fresh Weight ◽  
Available Nitrogen ◽  
Cotton Crop ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Increased Activity

SUMMARYThe potential of cyanobacteria-based compost formulations was evaluated in cotton crop at two agro-ecological locations (Nagpur and Sirsa) as plant growth promoting (PGP) and biocontrol agents. Compost-based formulations fortified withCalothrixsp. orAnabaenasp. enhanced germination and fresh weight of plants, and microbiological activity by 10–15%, besides increased available nitrogen (by 20–50%) in soil at Nagpur. In the fungi-infected fields at Sirsa,Anabaena–T. viridebiofilmed formulation performed the best, recording 11.1% lower plant mortality than commercialTrichodermaformulation. Scanning electron microscopy confirmed the colonisation of inoculated cyanobacteria/biofilms on roots. Significant correlation between mortality, increased activity of hydrolytic enzymes and fresh weight of plant roots were recorded.Calothrixsp. andAnabaenasp. proved promising as both PGP and biocontrol agents, while biofilmed formulations substantially reduced mortality of cotton plants in sick plots. This study illustrates the promise of cyanobacteria as viable inoculation option for integrated nutrient and pest management strategies of cotton.

Increased Activity of Wall Hydrolytic Enzymes May Explain the Phenotype of Saccharomyces cerevisiae Fragile Mutants

1998 ◽  
Vol 37 (6) ◽  
pp. 365-367 ◽  
Author(s):  
José Ruiz-Herrera ◽  
Claudia León-Ramírez ◽  
P. Elizabeth Alvarez ◽  
Pencho Venkov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hydrolytic Enzymes ◽  
Increased Activity

scholarly journals THE INFLUENCE OF PHAGOCYTOSIS ON THE INTRACELLULAR DISTRIBUTION OF GRANULE-ASSOCIATED COMPONENTS OF POLYMORPHONUCLEAR LEUCOCYTES

1960 ◽  
Vol 112 (6) ◽  
pp. 1015-1022 ◽  
Author(s):  
Zanvil A. Cohn ◽  
James G. Hirsch
Keyword(s):  
High Speed ◽  
Hydrolytic Enzymes ◽  
Supernatant Fraction ◽  
Polymorphonuclear Leucocytes ◽  
Progressive Decrease ◽  
Cytoplasmic Granules ◽  
Large Numbers ◽  
Significant Difference ◽  
Increased Activity ◽  
Total Enzyme

Control and phagocyting populations of rabbit polymorphonuclear leucocytes have been compared in terms of the content and distribution of phagocytin and selected hydrolytic enzymes. Following incubation at 37°C., the cells were disrupted by homogenization and separated into an 8,200 g pellet and supernatant fluid. The high speed pellet from control leucocytes contained all the larger particulate elements of the cell including intact cytoplasmic granules. The ingestion of large numbers of heat-killed bacteria was accompanied by a fourfold reduction in the total phagocytin content of the leucocyte, whereas phagocytosis did not influence the recovery of histone-like bactericidin from nuclei. Engulfment of microorganisms led to a progressive decrease in the activity of phosphatases, beta glucuronidase, and cathepsin extractable from the 8,200 g pellet, with a concomitant increased activity of these enzymes in the supernatant fraction. No significant difference in the total enzyme content of control and phagocyting cells was noted. These findings are consistent with the lysis of granules following phagocytosis and the liberation of granule constituents into the cytoplasm of the leucocyte.

scholarly journals Cutting Apple Fruits Induces Cellulase Activity in the Abscission Zone

HortScience ◽  
1999 ◽  
Vol 34 (4) ◽  
pp. 601-603 ◽  
Author(s):  
Daniel L. Ward ◽  
Eric P. Beers ◽  
Ross E. Byers ◽  
Richard P. Marini
Keyword(s):  
Thin Layer ◽  
Hydrolytic Enzymes ◽  
Cellulase Activity ◽  
Abscission Zone ◽  
Thin Layer Plate ◽  
Naphthaleneacetic Acid ◽  
Crop Loss ◽  
Increased Activity ◽  
Apple Fruits

Preharvest abscission of apple [Malus ×domestica (L.) Borkh.] fruits causes significant crop loss in many years. In this study, fruit cutting was used to induce abscission in August and September. Abscission zones of `Redchief Delicious' Mercier strain fruits were sampled 0, 2, 4, and 6 days after cutting. Thin-layer-plate assays were developed and used to identify hydrolytic enzymes active in the abscission zone (AZ) after induction. Increased activity of cellulase, but not polygalacturonase, was detected in the AZ following cutting. Cellulase activity was consistently high in AZs 4 days after cutting. Both AVG (652 mg·L–1) and NAA (10 mg·L–1) applied 2 or 4 days after cutting delayed drop, but NAA delayed drop 1.6 days longer than did AVG. Fruits treated with AVG dropped over a longer period than did control or NAA-treated fruits. Chemical names used: aminoethoxyvinylglycine (AVG); naphthaleneacetic acid (NAA).

Rhabdomyolysis in DOMS

1999 ◽  
Vol 38 (05) ◽  
pp. 164-168 ◽  
Author(s):  
Gloria Ruiz Hernandez ◽  
C. Sanchez Marchori ◽  
J. Munoz Moliner ◽  
C. Martinez Carsi
Keyword(s):  
Bone Scintigraphy ◽  
Previous History ◽  
Biochemical Studies ◽  
History Of ◽  
Increased Activity

SummaryA 26-year-old man with a previous history of external twin bursitis was remitted to our Department for a bone scintigraphy. Before the study, the patient performed an elevated number of intense sprints. Bone scintigraphy showed a bilaterally increased activity in both anterior rectum muscles suggesting rhabdomyolysis. Biochemical studies and MRT confirmed the diagnosis.

Increased Activity of Vitamin K-Dependent Clotting Factors in Human Hyperlipoproteinaemia – Association with Cholesterol and Triglyceride Levels

1977 ◽  
Vol 38 (02) ◽  
pp. 0465-0474 ◽  
Author(s):  
M Constantino ◽  
C Merskey ◽  
D. J Kudzma ◽  
M. B Zucker
Keyword(s):  
Vitamin K ◽  
Plasma Lipids ◽  
Coagulation Factors ◽  
Clotting Factor ◽  
Factor X ◽  
Clotting Factors ◽  
Blood Coagulation Factors ◽  
Cholesterol Levels ◽  
Type V ◽  
Increased Activity

SummaryLevels of blood coagulation factors, cholesterol and triglyceride were measured in human plasma. Prothrombin was significantly elevated in type Ha hyperlipidaemia; prothrombin and factors VII, IX and X in type lib; and prothrombin and factors VII and IX in type V. Multiple regression analysis showed significant correlation between the levels of these plasma lipids and the vitamin K-dependent clotting factors (prothrombin, factors VII, IX and X). Higher cholesterol levels were associated with higher levels of prothrombin and factor X while higher triglyceride levels were associated with higher levels of these as well as factors VII and IX. Prothrombin showed a significant cholesterol-triglyceride interaction in that higher cholesterol levels were associated with higher prothrombin levels at all levels of triglyceride, with the most marked effects in subjects with higher triglyceride levels. Higher prothrombin levels were noted in subjects with high or moderately elevated (but not low) cholesterol levels. Ultracentrifugation of plasma in a density of 1.21 showed activity for prothrombin and factors VII and X only in the lipoprotein-free subnatant fraction. Thus, a true increase in clotting factor protein was probably present. The significance of the correlation between levels of vitamin K-dependent clotting factors and plasma lipids remains to be determined.

PENERAPAN PENDEKATAN PEMBELAJARAN PAKEM DALAM MENINGKATKAN HASIL BELAJAR MATEMATIKA SISWA KELAS IV SDN 002 BAGAN BESAR

2017 ◽  
Vol 5 (3) ◽  
pp. 411
Author(s):  
Elvi Zahara
Keyword(s):  
Active Learning ◽  
Student Learning ◽  
Learning Outcomes ◽  
Mathematics Teaching ◽  
Mathematics Learning ◽  
Student Learning Outcomes ◽  
Average Value ◽  
Increased Activity

The background of this research is the lack of students' mathematics learning outcomes. Thelow yield caused by the learning of mathematics teaching is so very boring, monotonous,stressful and meaningless. Departing from the problems that occur in the field, especially ingrade IV SDN 002 Bagan Besar then there needs to be a strategy that can provide learningmathematics is expected to show active learning, creative, effective and fun. This research istindaan class (PTK), instrument collecting data observation and test learning outcomes. Theresults obtained: Increased activity of the students from the first cycle to the second cycle arevery significant. The increase in active learning, creative, and fun efekif the students alsofollowed by an increase in student learning outcomes. This is evidenced by the increase in thelearning outcomes of the first cycle to the second cycle. Namely, from the average value of 88,27 (cycle I) increased to an average value of 97, 59 (cycle II).

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