Irrigation with thapsigargin and various concentrations of 5-fluorouracil in a sealed-capsule irrigation device in young rabbit eyes to prevent after-cataract

An attempt has been made to determine the relative functional activities of adrenal and extra-adrenal chromaffin tissue during the neonatal phase and up to 1 week of age using young rabbits and guinea-pigs whose main extra-adrenal abdominal para-aortic chromaffin bodies are respectively non-innervated and innervated. Amine synthesis and storage were followed by assay and autoradiography after a single intraperitoneal injection of l-[2,5,6-3H]DOPA and the findings correlated with amine content as assessed by high performance liquid chromatography and by volume of tissue. The results indicate that in the guinea-pig, in spite of differences in proportions of adrenaline and noradrenaline in the adrenal gland and para-aortic body (PAB), the loss of labelled catecholamines from the innervated PAB closely follows that from the adrenal medulla, suggesting that both participate in normal sympathoadrenal activity. By comparison, in the rabbit the PAB shows only a minimal decrease in labelled amine during the first week of life during which period the amine content and concentration of the PAB doubles: the functional significance of this non-innervated extra-adrenal chromaffin tissue, which persists throughout life, has still to be determined.

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Sealed Capsule Irrigation Device

Encyclopedia of Ophthalmology ◽

10.1007/978-3-642-35951-4_375-5 ◽

2012 ◽

pp. 1-2

Author(s):

Tanja M. Rabsilber ◽

Gerd U. Auffarth

Keyword(s):

Sealed Capsule Irrigation

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A role for clonal dominance in the maintenance of allotype suppression?

Journal of Experimental Medicine ◽

10.1084/jem.150.4.888 ◽

1979 ◽

Vol 150 (4) ◽

pp. 888-897 ◽

Cited By ~ 8

Author(s):

A Yamada ◽

L T Adler ◽

F L Adler

Keyword(s):

Immunological Memory ◽

Young Rabbit ◽

Environmental Antigens

The establishment of immunological memory during the early and complete phase of allotype suppression in the young rabbit has been shown to lead to the preferential production of antibodies with the nonsuppressed allotypic specificity in response to recall injections given after spontaneous or induced release from suppression. It is suggested that this manifestation of clonal dominance, applied to stimulation by environmental antigens, may contribute to the long lasting persistence of allotype imbalance in allotype suppressed rabbits.

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Comparison of the development of proteolytic activity in the abomasum of the preruminant calf with that in the stomach of the young rabbit and guinea-pig

British Journal Of Nutrition ◽

10.1079/bjn19730034 ◽

1973 ◽

Vol 30 (2) ◽

pp. 285-296 ◽

Cited By ~ 20

Author(s):

M. J. Henschel

Keyword(s):

Guinea Pig ◽

Proteolytic Activity ◽

Post Partum ◽

Stomach Contents ◽

Young Rabbit ◽

Main Peak ◽

Adult Rabbit ◽

Guinea Pig Stomach ◽

High Level ◽

Protease Secretion

1. Proteolytic activity was measured in the abomasal contents of five calves during the first 7 weeks of life and in the stomach contents of rabbits and guinea-pigs during the first 4 weeks of life.2. The pattern of protease secretion in the abomasum varied considerably among individual calves; in addition to rennin, some pepsin activity was found in most animals from the first sampling at 9 d of age.3. Only pepsin was secreted in the guinea-pig stomach from birth, at a consistently high level at all ages.4. The main peak of proteolytic activity in the rabbit stomach during the 1st week post partum had an optimum at pH 3·5–4·0, corresponding to that for calf rennin. During the 3rd week of life the main pepsin peak, with an optimum at pH 1·6–2·0, developed.5. Agarose gel electrophoresis of an extract of neonatal rabbit stomach mucosa showed the activity peak at pH 3·5–4·0 to be due to two slow-moving components of mobilities similar to that of crystalline rennin. At least one of these cross-reacted with antiserum to crystalline rennin.6. At 10 d and 3 weeks of age, activity at pH 1·6–2·0 was due to two faster-moving proteases, one being the main pepsin of the adult rabbit, which moved at the same speed as crystalline porcine pepsin, with the antiserum of which it cross-reacted.

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