Lipid peroxidation, glutathione levels and changes in glutathione-related enzyme activities in streptozotocin-induced diabetic rats

This study investigated the effects of various concentrations and incubation time intervals of CDA-II (cell differentiation agent: a preparation of human urine) on cell viability, lipid peroxidation and glutathione and its related enzyme activities in rat hepatocytes. Based on the results of lactate dehydrogenase leakage and microscopic examination, treatment with CDA-II significantly elevated the viability of hepatocytes. The level of lipid peroxidation of cells treated with 2.5 or 5 mg/ml CDA-II was lower than the control after 4, 8 and 24 hours of incubation. Intracellular glutathione (GSH) content of cells treated with 0.25–5 mg/ml CDA-II was significantly higher than controls after 8 and 24 hours of incubation. These phenomena are beneficial to the antioxidant capabilities of hepatocytes. Furthermore, treatment of hepatocytes with CDA-II has no effect on the activities of GSH peroxidase, GSH reductase and GSH S-transferase. In conclusion, adequate concentration of CDA-II could enhance the viability and antioxidative capability of hepatocytes.

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Effect of Garlic Active Principle, Diallyl Disulfide, on Cell Viability, Lipid Peroxidation, Glutathione Concentration and its Related Enzyme Activities in Primary Rat Hepatocytes

The American Journal of Chinese Medicine ◽

10.1142/s0192415x99000124 ◽

1999 ◽

Vol 27 (01) ◽

pp. 95-105 ◽

Cited By ~ 19

Author(s):

Lee-Yan Sheen ◽

Shiow-Fen Sheu ◽

Shun-Jen Tsai ◽

Raymond H. C. Meng ◽

Chong-Kuei Lii

Keyword(s):

Lipid Peroxidation ◽

Cell Viability ◽

Enzyme Activities ◽

Rat Hepatocytes ◽

Active Principle ◽

Diallyl Disulfide ◽

Glutathione S Transferase ◽

Time Intervals ◽

Related Enzyme ◽

The Media

This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation. and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (p < 0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-S-transferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values.

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