Effect of CDA-II on Cell Viability, Lipid Peroxidation, Glutathione Concentration and Its Related Enzyme Activities in Primary Rat Hepatocytes

2003 ◽  
Vol 31 (03) ◽  
pp. 415-423 ◽  
Author(s):  
Wen-Chuan Lin ◽  
Yuan-Chang Liao ◽  
Ming-Cheng Liau ◽  
Lee-Yan Sheen
Keyword(s):  
Lipid Peroxidation ◽  
Cell Differentiation ◽  
Lactate Dehydrogenase ◽  
Cell Viability ◽  
Enzyme Activities ◽  
Rat Hepatocytes ◽  
Time Intervals ◽  
Intracellular Glutathione ◽  
Related Enzyme ◽  
Cda Ii

This study investigated the effects of various concentrations and incubation time intervals of CDA-II (cell differentiation agent: a preparation of human urine) on cell viability, lipid peroxidation and glutathione and its related enzyme activities in rat hepatocytes. Based on the results of lactate dehydrogenase leakage and microscopic examination, treatment with CDA-II significantly elevated the viability of hepatocytes. The level of lipid peroxidation of cells treated with 2.5 or 5 mg/ml CDA-II was lower than the control after 4, 8 and 24 hours of incubation. Intracellular glutathione (GSH) content of cells treated with 0.25–5 mg/ml CDA-II was significantly higher than controls after 8 and 24 hours of incubation. These phenomena are beneficial to the antioxidant capabilities of hepatocytes. Furthermore, treatment of hepatocytes with CDA-II has no effect on the activities of GSH peroxidase, GSH reductase and GSH S-transferase. In conclusion, adequate concentration of CDA-II could enhance the viability and antioxidative capability of hepatocytes.

Effect of Garlic Active Principle, Diallyl Disulfide, on Cell Viability, Lipid Peroxidation, Glutathione Concentration and its Related Enzyme Activities in Primary Rat Hepatocytes

1999 ◽  
Vol 27 (01) ◽  
pp. 95-105 ◽  
Author(s):  
Lee-Yan Sheen ◽  
Shiow-Fen Sheu ◽  
Shun-Jen Tsai ◽  
Raymond H. C. Meng ◽  
Chong-Kuei Lii
Keyword(s):  
Lipid Peroxidation ◽  
Cell Viability ◽  
Enzyme Activities ◽  
Rat Hepatocytes ◽  
Active Principle ◽  
Diallyl Disulfide ◽  
Glutathione S Transferase ◽  
Time Intervals ◽  
Related Enzyme ◽  
The Media

This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation. and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (p < 0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-S-transferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values.

Effect of hypoxic cell radiosensitizers on glutathione level and related enzyme activities in isolated rat hepatocytes

Life Sciences ◽  
1985 ◽  
Vol 37 (7) ◽  
pp. 625-633
Author(s):  
Kiyoshi Noguchi ◽  
Tatsuaki Hattori ◽  
Takashi Igarashi ◽  
Koichi Ueno ◽  
Tetsuo Satoh ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Rat Hepatocytes ◽  
Hypoxic Cell ◽  
Glutathione Level ◽  
Isolated Rat Hepatocytes ◽  
Related Enzyme ◽  
Isolated Rat

scholarly journals Study of rat hepatocytes in primary culture submitted to hypoxia and reoxygenation: action of the cytoprotectors prostaglandin E1, superoxide dismutase, allopurinol and verapamil

2009 ◽  
Vol 46 (4) ◽  
pp. 333-340 ◽  
Author(s):  
Dahir Ramos de Andrade Jr. ◽  
Dahir Ramos de Andrade ◽  
Sânia Alves dos Santos
Keyword(s):  
Superoxide Dismutase ◽  
Lactate Dehydrogenase ◽  
Cell Viability ◽  
Primary Culture ◽  
Prostaglandin E1 ◽  
Rat Hepatocytes ◽  
Cultured Hepatocytes ◽  
Hepatic Diseases ◽  
Hypoxia Reoxygenation

CONTEXT: Exposure of hepatocytes to pathological conditions in a microenvironment of hypoxia and reoxygenation is very frequent in hepatic diseases. Several substances present perspectives for cytoprotective action on hepatocyte submitted to reoxygenation after hypoxia and simple hypoxia. OBJECTIVE: We research therapeutic options for hepatocytes submitted to hypoxia and hypoxia + reoxygenation injury. METHODS: Primary culture of rat hepatocytes was submitted to hypoxia (2 hours) plus reoxygenation (2 hours) and simple hypoxia (4 hours) in the presence or the absence of cytoprotectors. The hepatocyte lesion was evaluated by functional criteria through percentage of lactate dehydrogenase released and cell viability. The effects of the cytoprotectors prostaglandin E1 3 ηg/mL, superoxide dismutase 80 μg/mL, allopurinol 20 μM and verapamil 10-4 M were studied in this model of injury. RESULTS: Reoxygenation after hypoxia induced more significant lesion in cultured hepatocytes compared to simple hypoxia, detected by analysis of functional criteria. There was a significant reduction of percentage of lactate dehydrogenase released and a significant increase of percentage of cell viability in the hypoxia + reoxygenation + cytoprotectors groups compared to hypoxia + reoxygenation groups. Prostaglandin E1, superoxide dismutase and verapamil also protected the group submitted to simple hypoxia, when evaluated by functional criteria. CONCLUSIONS: We conclude that reoxygenation after hypoxia significantly increased the lesion of cultured rat hepatocytes when compared to simple hypoxia. Prostaglandin E1, superoxide dismutase, allopurinol and verapamil acted as cytoprotectors to the rat cultured hepatocytes submitted to hypoxia + reoxygenation in vitro. The substances prostaglandin E1, superoxide dismutase and verapamil protected hepatocytes submitted to simple hypoxia on the basis of all the criteria studied in this experimental model.

Protective Effects of Water Extract of Clam on Normal and CCl4-Induced Damage in Primary Cultured Rat Hepatocytes

2010 ◽  
Vol 38 (06) ◽  
pp. 1193-1205 ◽  
Author(s):  
H.M. Chi ◽  
S.T. Chou ◽  
S.C. Lin ◽  
Z.Y. Su ◽  
Lee-Yan Sheen
Keyword(s):  
Lactate Dehydrogenase ◽  
Carbon Tetrachloride ◽  
Water Extract ◽  
Rat Hepatocytes ◽  
Control Group ◽  
Protective Effects ◽  
Primary Cultured Rat Hepatocytes ◽  
Defense Systems ◽  
Intracellular Glutathione ◽  
Cultured Rat Hepatocytes

The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4 -induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ~ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl4) for 1 hour, at concentrations of WEC between 0.42 ~ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4 -treated control group ( p < 0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.

In-vitro cytoprotective activity of Eichhornia crassipes ϐlowers against hydrogen peroxide induced oxidative stress in BRL 3A rat liver cells

2021 ◽  
Vol 12 (3) ◽  
pp. 1788-1802
Author(s):  
Rajarajan S ◽  
Sivakrishnan S ◽  
Ganesan V
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Lactate Dehydrogenase ◽  
Cell Viability ◽  
Eichhornia Crassipes ◽  
Ethanol Extract ◽  
Oxidative Injury ◽  
Cytoprotective Activity

The aim of this study was to see whether an ethanol extract of Eichhornia crassipes flowers and its fractions could protect BRL 3A liver cells from hydrogen peroxide-induced oxidative stress. Eichhornia crassipes powdered flowers were subjected to a hot continuous extraction in a soxhlet extractor using ethanol as the solvent material. The solvent extracts were first tested for in-vitro free radical scavenging and anti-oxidant activity using qualitative and quantitative methods. Benzene, chloroform, and n-butanol were used to fractionate the ethanol extract. In BRL 3A cell lines, the crude ethanol extract and its fractions were tested for their possible cytoprotective effect against hydrogen peroxide (H2O2) induced oxidative stress. Cell viability, lipid peroxidation by measuring the formation of malondialdehyde, lactate dehydrogenase leakage into culture medium, catalase activity, and the content of reduced glutathione (GSH) in the cells were all tested in biochemical assays to determine the cytoprotective activity. BRL 3A cells were exposed to 2mM H2O2, which decreased cell viability, increased malondialdehyde (MDA) levels, increased lactate dehydrogenase (LDH) leakage, and reduced antioxidant activities. Pretreatment of cultured cells for 30 minutes with crude ethanol extract of Eichhornia crassipes flowers and various solvent fractions at concentrations of 0.01, 0.1, 1, 10, 100 g/ml attenuated oxidative injury in a dose-dependent manner until H2O2 exposure. The crude ethanol extract of Eichhornia crassipes flowers was found to have a strong cytoprotective impact, raising cell viability while decreasing lipid peroxidation and LDH leakage. In the cells pre-treated with ethanol extract of Eichhornia crassipes flowers, there was a further increase in catalase and a decrease in glutathione activity. These results indicate that an ethanol extract of Eichhornia crassipes flowers has potent cytoprotective properties against reactive oxygen species-induced oxidative injury.

Effects of the Deer Antler Extract on Scopolamine-induced Memory Impairment and Its Related Enzyme Activities

2009 ◽  
Vol 38 (4) ◽  
pp. 409-414 ◽  
Author(s):  
Mi-Ra Lee ◽  
Bai-Shen Sun ◽  
Li-Juan Gu ◽  
Chun-Yan Wang ◽  
Zhe-Ming Fang ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Memory Impairment ◽  
Deer Antler ◽  
Related Enzyme
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