The Genetic Engineering of Two Commercial Potato Cultivars for Resistance to Potato Virus X

Virus and fungal resistance traits are important targets in the genetic engineering of agricultural and horticultural crops. We have engineered resistance against potato virus X in important commercial potato cultivars. Four years of field trials with resistant potatoes have demonstrated the commercial feasibility of improving potato cultivars by selectively adding new traits while preserving intrinsic properties. In our pursuit for a broad resistance against fungi we have focused on the exploitation of genes encoding antifungal proteins. We present results demonstrating the antifungal effect of some of these proteins in vitro , as well as the synergy between specific chitinases and β-1,3-glucanases. We also report high level resistance against Fusarium oxysporum in transgenic tomato plants expressing a specific combination of genes encoding these enzymes.

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Occurrence of potato virus X strain group 1 in seed stocks of potato cultivars lacking resistance genes

Annals of Applied Biology ◽

10.1111/j.1744-7348.1995.tb07606.x ◽

1995 ◽

Vol 127 (3) ◽

pp. 479-487 ◽

Cited By ~ 7

Author(s):

C R. Wilson ◽

R A C. Jones

Keyword(s):

Potato Virus ◽

Resistance Genes ◽

Potato Virus X ◽

Potato Cultivars ◽

Strain Group ◽

Group 1

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Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype

Phytopathology Research ◽

10.1186/s42483-021-00099-6 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Jiahui Liu ◽

Ye Liu ◽

Yue Fang ◽

Lili Zhang ◽

Kaixin Yu ◽

...

Keyword(s):

Potato Virus ◽

Fluorescent Protein ◽

Infectious Clone ◽

Yellow Fluorescent Protein ◽

Potato Virus X ◽

Potato Production ◽

Potato Cultivars ◽

Major Type ◽

Long Distance ◽

Viral Pathogen

AbstractPotato virus X (PVX) is a widely distributed viral pathogen that causes significant losses in potato production by co-infecting with potato virus Y or potato virus A. In this study, the resistance of 23 potato cultivars to PVX was dissected in detail using a PVX infectious clone containing a yellow fluorescent protein (YFP). Among them, four potato cultivars (Longshu-3, Eugene, Atlantic and Waiyin-2) were found to carry an Rx gene that confers extreme resistance to PVX; one cultivar (Waiyin-1) displayed partial resistance and was able to delay PVX infection by ~ 5 days; while the rest eighteen potato cultivars were susceptible to PVX. Moreover, we found that the replication but not cell-to-cell or long-distance movement of PVX was inhibited in Waiyin-1. Finally, we determined that the expression of pathogenesis-related (PR) genes in Waiyin-1 was not triggered by PVX infection at early infection stage, whereas they were triggered in the Rx-carrying cultivar Atlantic during this period of time. In conclusion, our results confirm that Rx is a major type of resistance gene in potato cultivars in the Northeast part of China. Furthermore, the possible mechanism underlying Waiyin-1 resistance to PVX is discussed.

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Resistance to potato leafroll virus infection and accumulation in potato cultivars, and the effects of previous infection with other viruses on expression of resistance

Australian Journal of Agricultural Research ◽

10.1071/ar9931891 ◽

1993 ◽

Vol 44 (8) ◽

pp. 1891 ◽

Cited By ~ 24

Author(s):

CR Wilson ◽

RAC Jones

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

Potato Cultivars ◽

Infected Leaf ◽

Potato Virus S ◽

Breeding Lines ◽

Infection Pressure ◽

Previous Infection ◽

Leafroll Virus

A selection of potato cultivars and breeding lines was evaluated for presence of resistance to infection with potato leafroll virus (PLRV) via viruliferous aphid vectors ( IR) and/or resistance to accumulation of PLRV antigen ( AR) in infected leaf tissue. Cultivars Aracy, Delcora, Omega and Spunta, and breeding lines BR63.15 and B71.240.2 carried both IR and AR , Bismark, Serrana INTA and L/T1 had alone and Delaware had AR alone. The other cultivars tested had neither. Within both the resistant and susceptible classes for AR, the level of PLRV antigen accumulation achieved varied with cultivar. Previous infection with potato virus X (PVX) or potato virus S (PVS) either alone or together did not diminish the expression of IR or AR. However, the presence of PVX sometimes significantly increased the accumulation of PLRV in susceptible cv. Desiree and this effect was most pronounced in mature leaves of older plants. In contrast, presence of PVX in susceptible cv. Desiree did not increase the numbers of plants becoming infected with PLRV. Identifying potato genotypes with and IR AR will help the Australian potato industry to select cultivars which become infected with PLRV more slowly under conditions of high infection pressure and/or are suitable for use as parental lines in breeding virus-resistant cultivars.

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