scholarly journals Identification of epigenetic interactions between microRNA and DNA methylation associated with polycystic ovarian syndrome

2020 ◽  
Vol 66 (2) ◽  
pp. 123-137 ◽  
Author(s):  
Zhanrui Mao ◽  
Ting Li ◽  
Hui Zhao ◽  
Yulan Qin ◽  
Xuesong Wang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Polycystic Ovarian Syndrome ◽  
Ovarian Syndrome

scholarly journals Novel HumanMethylatyion450 Analyses Reveal DNA Methylation Predictors of Future Diabetes Onset in Women With Polycystic Ovarian Syndrome

2021 ◽  
Author(s):  
Mark Kitchen ◽  
Ayesha Manzur ◽  
Anthony Fryer ◽  
Pensee Wu
Keyword(s):  
Dna Methylation ◽  
High Risk ◽  
Polycystic Ovarian Syndrome ◽  
Good Health ◽  
High Risk Population ◽  
Diabetes Onset ◽  
Increased Risk ◽  
Illumina Humanmethylation450 Beadchip ◽  
Ovarian Syndrome ◽  
Future Diabetes

Abstract Aims. Polycystic ovarian syndrome (PCOS) is a common endocrine disorder characterised by ovarian morphological, systemic biochemical, and menstrual changes. Women with PCOS are at significantly increased risk of raised fasting glucose, impaired glucose tolerance, and diabetes. Recognition of these complications and early intervention are key to good health outcomes. We sought to identify DNA methylation patterns that may predict future diabetes onset in this high-risk PCOS population. Patients and Methods. Peripheral blood samples from women with PCOS and women with PCOS who later developed diabetes, were analysed by Illumina HumanMethylation450 BeadChip-arrays. Bisulphite-Pyrosequencing™ was used to validate and confirm array methylation data. Results. Array analyses identified 273 differentially methylated CpG loci (≥ 0.2 β-value change) at initial diagnosis of PCOS, between women who did or did not later develop diabetes. 19 of these sites demonstrated differential methylation in the same direction in ≥ five sample pairs. Methylation in three of the candidates (cg11897887, cg02819655, and cg25542007) showed the best concordance with corresponding array β-values, and, most clearly differentiated ‘cases’ from ‘controls’. Conclusions. We have identified novel methylation biomarkers that could predict future onset of diabetes in this high-risk population. Use of methylation analyses to identify women who are likely to develop diabetes at diagnosis of PCOS may facilitate timely lifestyle interventions to reduce future morbidity.

scholarly journals Identification of Epigenetic Interactions between MicroRNA and DNA Methylation Associated with Polycystic Ovarian Syndrome

2019 ◽  
Author(s):  
Zhanrui Mao ◽  
Ting Li ◽  
Hui Zhao ◽  
Yulan Qin ◽  
Yani Kang
Keyword(s):  
Dna Methylation ◽  
Mirna Expression ◽  
Polycystic Ovarian Syndrome ◽  
Promoter Hypermethylation ◽  
Integrated Analysis ◽  
Rna Seq ◽  
Promoter Regions ◽  
Potential Therapeutic Target ◽  
And Control ◽  
Ovarian Syndrome

Aberration in microRNA (miRNA) expression or DNA methylation is a causal factor for polycystic ovarian syndrome (PCOS), a common endocrine disorder and leading cause of infertility. However, the epigenetic interactions between miRNA and DNA methylation remain unexplored in PCOS. In this study, we conducted an integrated analysis of RNA-seq, miRNA-seq and MBD-seq on ovarian granulosa cells of PCOS and control groups to reveal the epigenetic interactions involved in the pathogenesis of PCOS. Firstly, we identified 830 genes and 30 miRNAs that were expressed differently in PCOS, and seven miRNAs were found to negatively regulate targeted mRNA expression. Next, in total, 130 miRNAs were found to be significantly differently methylated in promoter regions, while 13 were found to be associated with miRNA expression. Furthermore, the promoter hypermethylation of miR-429, miR-141-3p, and miR-126-3p was proven to suppress miRNA expression and therefore upregulate their corresponding genes, including XIAP, BRD3, MAPK14 and SLC7A5. Our results demonstrate that DNA methylation regulates miRNA expression and therefore controls its corresponding gene expression. The reactivation of the transcription of epigenetically silenced genes may be one of the key elements in PCOS pathogenesis. Meanwhile, the epigenetic mechanisms underlying the regulation of miRNA expression can provide a potential therapeutic target for PCOS in the future.

scholarly journals Differential DNA methylation patterns of polycystic ovarian syndrome in whole blood of Chinese women

Oncotarget ◽  
2016 ◽  
Vol 8 (13) ◽  
pp. 20656-20666 ◽  
Author(s):  
Shuxia Li ◽  
Dongyi Zhu ◽  
Hongmei Duan ◽  
Anran Ren ◽  
Dorte Glintborg ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Whole Blood ◽  
Polycystic Ovarian Syndrome ◽  
Chinese Women ◽  
Methylation Patterns ◽  
Ovarian Syndrome

Characteristics of Polycystic Ovarian Syndrome in Sub-fertile Woman Attending to GOPD in ShSMCH

2019 ◽  
Vol 11 (1) ◽  
pp. 17-23
Author(s):  
Jinnat Ara Islam ◽  
Fatema Ashraf ◽  
Eva Rani Nandi
Keyword(s):  
Polycystic Ovarian Syndrome ◽  
Polycystic Ovaries ◽  
Cross Sectional ◽  
Medical College ◽  
Appropriate Treatment ◽  
Pcos Group ◽  
Symptom Complex ◽  
Ovarian Syndrome ◽  
Selection Of ◽  
Fertile Women

Background: Polycystic ovarian syndrome (PCOS) is a condition characterized by menstrual abnormalities (oligo/amenorrhea) and clinical or biochemical features of hyperandrogenism and may manifest at any age. It is a common cause of female subfertility. All the dimensions of PCOS have not been yet completely explored. Methods: It was a cross sectional comparative study carried out at-GOPD of Shaheed Suhrawardy Medical College & Hospital from January, 2016 to December 2016 on 162 subfertile women. Among them 54 were PCOS group and 108 were non PCOS group. PCOS was diagnosed by (Rotterdam criteria 2003) (i) Oligo or anovulation (ii) hyperandrogenism (iii) Polycystic ovaries. Study was done to evaluate and compare the demographic characteristics, clinical, biochemical and ultrasoundgraphic features of sub-fertile women with and without PCOS. Results: A total of 162 sub-fertile women aged 16-36 years. Mean age was 29.5±5.4. There were significant differences between the two groups in terms of (oligo/amenorrhea), hirsutism, WHR and ovarian ultrasound features. There were no significant differences between two groups in correlations between the level of obesity with the incidence of anovulation, hyperandrogenism or with hormonal features. Conclusion: PCOS is one of the important factors causing Infertility. It is an ill-defined symptom complex needed due attention. There is a need to increase awareness regarding. The clinical features of PCOS are heterogenous thus can be investigated accordingly of selection of appropriate treatment modality. J Shaheed Suhrawardy Med Coll, June 2019, Vol.11(1); 17-23

Impaired DHEA sulfation defines androgen excess in women with polycystic ovarian syndrome (PCOS)

2016 ◽  
Author(s):  
Punith Kempegowda ◽  
Michael W O'Reilly ◽  
Zaki Hassan-Smith ◽  
Karl-Heinz Storbeck ◽  
Angela E Taylor ◽  
...  
Keyword(s):  
Polycystic Ovarian Syndrome ◽  
Androgen Excess ◽  
Ovarian Syndrome

Visceral fat drives 5[alpha]-reductase activity independent of BMI in women with polycystic ovarian syndrome

2015 ◽  
Author(s):  
Punith Kempegowda ◽  
Michael W O'Reilly ◽  
Nicola J Crabtree ◽  
Angela E Taylor ◽  
Beverly A Hughes ◽  
...  
Keyword(s):  
Visceral Fat ◽  
Polycystic Ovarian Syndrome ◽  
Reductase Activity ◽  
Ovarian Syndrome
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